Production, medium optimization, and structural characterization of an extracellular polysaccharide produced by<i>Rhodotorula minuta ATCC 10658</i>

Samadlouie, Hamid Reza; Jahanbin, Kambiz; jalali, Parisa

doi:10.1002/fsn3.1792

Cited by 6 publications

(4 citation statements)

References 35 publications

(38 reference statements)

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“…Exopolysaccharide from Rhodotorula mucilaginosa CICC 33013 was composed of galactose, arabinose, glucose, and mannose ( 11 ). EPS of R. minuta ATCC 10658 consisted of mannose and glucose at a molar ratio of about 1.4:7.7 ( 29 ). The compositions of exopolysaccharides from a new cold-adapted R. mucilaginosa sp.…”

Section: Resultsmentioning

confidence: 99%

Prebiotic, Antioxidant, and Immunomodulatory Properties of Acidic Exopolysaccharide From Marine Rhodotorula RY1801

et al. 2021

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In this study, an extracellular acidic polysaccharide (EAPS) from marine Rhodotorula sp. RY1801 was extracted, and its biological properties were investigated. EAPS is mainly composed of monosaccharides, including mannose, rhamnose, glucose, galactose, and fucose, had an average molecular weight of 5.902 × 107 Da. The results indicated that EAPS can promote the growth of Lactobacillus acidophilus and L. acidophilus plantarum. EAPS is capable of scavenging both superoxide anion and hydroxyl radicals in vitro. The highest scavenging rate of superoxide anion and hydroxyl radicals is 29 and 84%, respectively. Using in vivo model, we found that the EAPS can expand the lifespan and increase the disease resistance of Caenorhabditis elegans against Klebsiella pneumoniae infection via the DAF-2/DAF-16 pathway. These results suggested that EAPS from marine Rhodotorula sp. RY1801 could promote the growth of beneficial bacteria and can be used as an antioxidant and immunomodulator, which had considerable potential in the food and health industry.

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Section: Resultsmentioning

confidence: 99%

Prebiotic, Antioxidant, and Immunomodulatory Properties of Acidic Exopolysaccharide From Marine Rhodotorula RY1801

et al. 2021

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“…Carbon sources are important factors determining the amount and the physicochemical characteristics of bacterial EPS [44]. Starch was ranked as the best substrate for extracellular polysaccharide production from Rhodotorula minuta ATCC 10,658 [65], as well as for Streptomyces sp. MOE6 in this work.…”

Section: Discussionmentioning

confidence: 99%

Bioremediation Potential of Streptomyces sp. MOE6 for Toxic Metals and Oil

et al. 2021

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Toxic metal contamination has serious effects on human health. Crude oil that may contain toxic metals and oil spills can further contaminate the environment and lead to increased exposure. This being the case, we chose to study the bio-production of inexpensive, environmentally safe materials for remediation. Streptomyces sp. MOE6 is a Gram-positive, filamentous bacterium from soil that produces an extracellular polysaccharide (MOE6-EPS). A one-factor-at-a-time experiments showed that the maximum production of MOE6-EPS was achieved at 35 °C, pH 6, after nine days of incubation with soluble starch and yeast extract as carbon sources and the latter as the nitrogen source. We demonstrated that MOE6-EPS has the capacity to remove toxic metals such as Co(II), Cr(VI), Cu(II) and U(VI) and from solution either by chelation and/or reduction. Additionally, the bacterium was found to produce siderophores, which contribute to the removal of metals, specifically Fe(III). Additionally, purified MOE6-EPS showed emulsifying activities against various hydrophobic substances, including olive oil, corn oil, benzene, toluene and engine oil. These results indicate that EPS from Streptomyces sp. MOE6 may be useful to sequester toxic metals and oil in contaminated environments.

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“…The solution was deproteinated by sevag method (1-butanol: chloroform at a ratio of 1:4, v/v; Staub, 1965 ). The sevag reagent was removed and a vacuum rotary evaporator (Heidolph Laborota, Germany) was employed to remove 2/3 water phase (supernatant), and then dialyzed against deionized water for 48 h. The concentrated water-soluble polysaccharides were precipitated by using 96% ethanol maintained at 4°C for 24 h. The precipitated polysaccharides were collected by centrifugation, and a vacuum Alpha 1–2 freeze-dryer (Christ, Germany) was used to attain crude G. lucidum polysaccharides (CGLP; Samadlouie et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Optimization of chemical conditions for metabolites production by Ganoderma lucidum using response surface methodology and investigation of antimicrobial as well as anticancer activities

Tajik,

Samadlouie,

Salek Farrokhi

et al. 2024

Front. Microbiol.

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Ganoderma lucidum (G. lucidum) is a medicinal mushroom that is known for its ability to produce compounds with physiological effects on human health. This research was undertaken to amplify the production of bioactive components of G. lucidum under optimal cultivation conditions, obtained in a submerged state and utilized in solid state fermentation, with the purpose of enhancing antimicrobial and anticancer activities. The results indicated that titanium dioxide (TiO2 NPs), magnesium oxide nanoparticles (MgO2 NPs), and B6, along with glucose syrup and CLS syrups, were the most effective for producing GA, while wheat starch and whey protein, along with MgO2 NPs and B6 vitamin, stimulated polysaccharide production using the One Factor at a Time (OFAT) method. After screening, the response surface method (RSM) statistically indicated that the media containing 42.11 g/L wheat starch with 22 g/L whey protein and 50 g/L glucose syrup with 30 g/L CSL were found to be the best conditions for polysaccharide (21.47% of dry weight biomass) and GA (20.35 mg/g dry weight biomass) production, respectively. The moss of the fruit body of G. lucidum produced under optimal GA conditions had the highest diversity in flavonoids and phenolic acids and significant antimicrobial activity against Esherichia coli (E. coli) and Bacillus subtilis (B. subtilis). In addition, the IC50 levels of shell and stem of G. lucidum were 465.3 and 485.7 μg/mL, respectively, while the moss did not reach 50% inhibition. In the end, the statistical approaches utilized in this research to elevate the levels of bioactive components in the fruiting body of G. lucidum produced a promising natural source of antimicrobial and anticancer agents.

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Production, medium optimization, and structural characterization of an extracellular polysaccharide produced byRhodotorula minuta ATCC 10658

Cited by 6 publications

References 35 publications

Prebiotic, Antioxidant, and Immunomodulatory Properties of Acidic Exopolysaccharide From Marine Rhodotorula RY1801

Prebiotic, Antioxidant, and Immunomodulatory Properties of Acidic Exopolysaccharide From Marine Rhodotorula RY1801

Bioremediation Potential of Streptomyces sp. MOE6 for Toxic Metals and Oil

Optimization of chemical conditions for metabolites production by Ganoderma lucidum using response surface methodology and investigation of antimicrobial as well as anticancer activities

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