Production, characterization, and species specificity of five monoclonal antibodies to Mycobacterium tuberculosis

Abstract

The production and characterization of five monoclonal antibodies to Mycobacterium tuberculosis are described. Specificity of the monoclonal antibodies was tested against other mycobacterial species by an enzyme-linked immunosorbent assay and immunoblots. HGT 3a, an immunoglobulin M (IgM) antibody, recognizes a molecule of 38,000 molecular weight present only in the tuberculosis complex of M. tuberculosis and Mycobacterium bovis BCG. HGT 6, an IgGl antibody, recognized two molecules with molecular weights of 43,000 and 45,000 and showed limited cross-reactivity. Three other antibodies, HGT 1, HGT 2, and HGT 4, all belonging to the IgGl type, recognized multiple bands and showed broad reactivity among all mycobacterial antigens tested, Escherichia coli, and Nocardia asteroids.

Isolation of a 43 kDa protein from Mycobacterium tuberculosis H37Rv and its identification as a pyridine nucleotide transhydrogenase

“…bovis BCG and not with Myco. bovzs (Kadival and Chaparas 1987), whereas it was observed here that TB43 could be isolated from Myco. bovis but not from Myco.…”

“…A 43-45 kDa CS protein of Myco. tuberculosis is recognized by MAb H G T 6 (Kadival and Chaparas 1987;Coates et al 1989). The reactivity of TB43 with H G T 6 was not checked.…”

Isolation and characterization of recombinant lambda gt11 bacteriophages expressing four different Mycobacterium intracellulare antigens

“…All 13 of the mycobacterial sonic extracts tested reacted with the HGT-6 antibody. At least three common mycobacterial antigens, of 12,65, and 70/71 kDa, have been shown to have structural homologies with known heat shock proteins (24). DNA and protein sequencing will be required to assess whether the 54-kDa antigen recognized by the HGT-6 antibody is also a stress protein.…”

Development of sensitive methods for the detection of myobacteria by DNA probes

“…The routine labora- tory isolation of mycobacteria is still complex and requires 8 weeks or longer. Monoclonal antibodies can detect mycobacteria rapidly but with low specificity [4]. DNA hybridization probe test is another possible tool for detecting mycobaeteria rapidly.…”