Development of sensitive methods for the detection of myobacteria by DNA probes

Abstract

A sensitive method allowing the detection of myobacteria by DNA probing has been developed. Besides the choice of a relevant probe encoding for part of the ribosomal RNA genes, the critical step allowing this high sensitivity was the method by which mycobacteria were lysed. Sonication of mycobacteria in the presence of chloroform followed by dot blot by this lysate gave the highest sensitivity in the detection of sequences homologous to the DNA probes.

Identification of mesophilic lactic acid bacteria by using polymerase chain reaction-amplified variable regions of 16S rRNA and specific DNA probes

“…In recent years, the use of rRNA sequences for identification and phylogenetic analysis has been generally accepted (1,5). DNA probes based on highly variable rRNA regions have been applied successfully for the identification and detection of microorganisms in soil, intestinal tract, and clinical samples (6,12,16). By comparing the published 16S rRNA sequences of Lactococcus spp.…”