Production and Verification of Hydrangea arborescens `Dardom' × H. involucrata Hybrids

Jones, Keri; Reed, Sandra M.

doi:10.21273/hortsci.41.3.564

Cited by 16 publications

(16 citation statements)

References 10 publications

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“…Phenotypes intermediate to the parents were also revealed in interspecific hybrids of H. arborescens 9 H. involucrata and of H. scandens 9 H. macrophylla, concerning leaf morphology (Jones and Reed 2006;Kudo et al 2008). Nevertheless, the diameter of inflorescences of H2 and H3 is significantly greater than that of the parents (Mann-Whitney test, P \ 0.05), suggesting a transgressive effect probably due to the heterosis effect induced by hybridization, as it has been shown by Kudo et al (2008) for a hybrid of H. scandens 9 H. macrophylla.…”

Section: Discussionmentioning

confidence: 89%

Architectural and genetic characterization of Hydrangea aspera subsp. aspera Kawakami group, H. aspera subsp. sargentiana and their hybrids

Crespel

Morel²,

Galopin

2011

Euphytica

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Hydrangea macrophylla (Thunb.) Ser. and H. paniculata Sieb. are the two most economically important species within the genus Hydrangea, and have been used as ornamental garden plants for a long time. However, other species such as H. aspera D. Don are of horticultural interest, particularly for the color of their inflorescences and plant shape. This species is composed of four sub-species and has previously been characterized both genetically and morphologically. The previous morphological characterization was qualitative, but was based mainly on leaf and inflorescence parameters outlined by UPOV, and provided little information about plant shape. To better characterize the shape of H. aspera, an architectural analysis was applied to the two most distantly related sub-species at the cytogenetic level:subsp. sargentiana (Redher) E.M. McClint. (clone 188) and subsp. aspera Kawakami group (clone 352). This method made it possible to reveal significant differences between these clones, both at the axis and the growth unit (GU) scale, in agreement with the high level of genetic differentiation (Jaccard dissimilarity index equal to 0.97) revealed between the two clones by Inter simple sequence repeats markers. Because this method is difficult to apply to a large population of individuals, a qualitative architectural characterization was tested on ten progenies derived from hybridization of the two clones, on the basis of their most discriminating architectural components. The hybrid nature of the progeny was confirmed by the architectural analysis. The architectural components of the hybrids are therefore a combination of those of the parents, with a predominance of clone 352, the female parent. Architectural differences between hybrids were clearly revealed by the length of the first vegetative GU (VGU1), the presence or the absence of VGU2 and the length of the floral GU of the A2 axis, and GU branching, allowing us to define five architectural profiles. These differences are supported by the average Jaccard dissimilarity index (0.33). This method, based on a qualitative description of the main architectural components of the plant, proved to be useful for characterizing the shape of H. aspera subsp. sargentiana, and subsp. aspera Kawakami group, and their hybrids. It could be extended to other sub-species of H. aspera and to their respective hybrids, providing an efficient L. Crespel (

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Section: Discussionmentioning

confidence: 89%

Architectural and genetic characterization of Hydrangea aspera subsp. aspera Kawakami group, H. aspera subsp. sargentiana and their hybrids

Crespel

Morel²,

Galopin

2011

Euphytica

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“…Data from pollinations involving cultivars later found to be triploids were analyzed for the following: percent polli-nations producing seed, mean number of seed per successful pollination, and percent seed germination. Previously published pollination and seed germination procedures were used (Jones and Reed, 2006;Reed, 2005).…”

Section: Methodsmentioning

confidence: 99%

“…For example, the Asiatic species H. involucrata (2n = 2x = 30) has approximately twice the nuclear DNA content of the American species H. arborescens (2n = 2x = 38) although both are diploid species (Zonneveld, 2004). A considerable difference in chromosome size between these two species was strikingly apparent in their hybrid (Jones and Reed, 2006).…”

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confidence: 96%

Analysis of Ploidy Level and Its Effects on Guard Cell Length, Pollen Diameter, and Fertility in Hydrangea macrophylla

Jones¹,

Reed²,

Rinehart³

2007

horts

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Ploidy level was estimated in Hydrangea macrophylla (Thunb) Ser. using flow cytometry. For H. macrophylla ssp. macrophylla, 42 diploid and 19 triploid cultivars were identified. All 14 H. macrophylla ssp. serrata (Thunb.) Makino cultivars tested were diploids. Somatic chromosome counts confirmed the ploidy of three diploid (2n = 2x = 36) and three triploid (2n = 3x = 54) cultivars. Stomatal guard cell length and pollen diameter of H. macrophylla ssp. macrophylla diploid cultivars were smaller than those of triploid cultivars. However, because the range of measurements for the diploids overlapped that of the triploids, neither guard cell nor pollen measurements are recommended for determining ploidy of H. macrophylla cultivars. Fertility was estimated using pollen staining and controlled pollinations. Stainable pollen for triploid cultivars averaged 63% and ranged from 25% in ‘Masja’ to 85% in ‘Marechal Foch’. Viable seed was obtained when four triploid cultivars were used as pistillate or staminate parents in controlled pollinations to diploid H. macrophylla ssp. macrophylla cultivars. A bimodal distribution of pollen sizes, which is suggestive of unreduced gamete production, was observed in one cultivar; however, more detailed genetic and cytologic studies are needed to elucidate the mechanism behind triploid formation in H. macrophylla taxa.

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“…Within the Hydrangea genus molecular markers also revealed a large diversity among the different species (Rinehart et al. 2005, Jones and Reed 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Phylogenetic analysis based on morphology (Hufford 1997, Mortreau 2003, plastid DNA restriction sites (Downie and Palmer 1992), sequences of the plastid genes matK and rbcL (Hufford et al 2001) and combinations of plastid and nuclear DNA (Soltis et al 2000) have provided valuable insights into the evolution of and the relationships in Hydrangeaceae. Within the Hydrangea genus molecular markers also revealed a large diversity among the different species (Rinehart et al 2005, Jones andReed 2006).…”

Section: Introductionmentioning

confidence: 99%

Karyotype analysis and physical mapping of 45S rRNA genes in Hydrangea species by fluorescence in situ hybridization

Laere¹,

Huylenbroeck²,

Bockstaele³

2008

Plant Breeding

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Detailed karyotypes of Hydrangea macrophylla, Hydrangea paniculata and Hydrangea quercifolia were constructed on the basis of arm lengths and centromeric index, together with 45S rDNA fluorescence in situ hybridization. Although the chromosomes were small, they were well distinguishable for all species. Chromosome morphology and karyotypes were different for the three species. H. macrophylla had six metacentric (M), eight submetacentric (SM) and four subtelocentric (ST) chromosomes. The karyotype of H. paniculata contained seven M, 10 SM and one ST chromosomes and H. quercifolia had six M, 10 SM and two ST chromosomes. The variability among three species also was expressed by 45S rDNA signals. H. macrophylla had a nucleolar organizing region on chromosome 2, H. paniculata had 45S rDNA signals on chromosomes 2, 5 and 11 and H. quercifolia on chromosomes 3 and 8. Hybridization signal always was distally on the short arm but the strength of the signals was different for the three species. The chromosome portraits made in this study will be used to trace chromosome behaviour in interspecific hybrids resulting from breeding work between the three species

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Production and Verification of Hydrangea arborescens `Dardom' × H. involucrata Hybrids

Cited by 16 publications

References 10 publications

Architectural and genetic characterization of Hydrangea aspera subsp. aspera Kawakami group, H. aspera subsp. sargentiana and their hybrids

Architectural and genetic characterization of Hydrangea aspera subsp. aspera Kawakami group, H. aspera subsp. sargentiana and their hybrids

Analysis of Ploidy Level and Its Effects on Guard Cell Length, Pollen Diameter, and Fertility in Hydrangea macrophylla

Karyotype analysis and physical mapping of 45S rRNA genes in Hydrangea species by fluorescence in situ hybridization

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