Analysis of Ploidy Level and Its Effects on Guard Cell Length, Pollen Diameter, and Fertility in Hydrangea macrophylla

Jones, Keri; Reed, Sandra M.; Rinehart, Timothy A.

doi:10.21273/hortsci.42.3.483

Cited by 33 publications

(46 citation statements)

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“…genome size estimates presented herein likely slightly overestimate the total nuclear DNA content of the H. macrophylla we tested (Zonneveld, 2004;Jones et al, 2007). The number of offspring that produced flowers ranged from 0% for 3x × 3x crosses to 73% for 2x × 2x crosses ( Table 5).…”

Section: Genome Size and Pollen Viability Of Seedlingsmentioning

confidence: 86%

“…In interploidy hybridizations, the parental genome contributions are altered, which affects endosperm development and the formation of viable seeds (Schatlowski and Köhler, 2012). Jones et al (2007) reported that mean number of seed per cross and percent seed germination were higher when triploid cultivars were used as the pistillate (female) rather than the staminate (male) parent in controlled crosses. Results herein indicated that not only does parental ploidy influence seed set and seed germination, but that parental influence continues throughout plant growth and flowering.…”

Section: Seed Set Germination and Seedling Growthmentioning

confidence: 99%

“…(Funamoto and Tanaka, 1988). All H. macrophylla cultivars tested to date are diploid (2n=2x=36) or triploid (2n=3x=54; Demilly et al, 2000;Cerbah et al, 2001;Zonneveld, 2004;Jones et al, 2007). Alexander (2017) showed that triploid hydrangeas had thicker stems, larger flowers, and larger stoma compared to full-sibling diploids.…”

Section: Introductionmentioning

confidence: 99%

“…Stainable pollen for the diploid and triploid H. macrophylla ssp. macrophylla cultivars averaged 70% and 63%, respectively, and results of controlled pollinations indicate that triploid cultivars can produce viable seed (Jones et al, 2007). Breeding improvement using mixed ploidy levels requires the adoption of cytological methods to assess fertility of potential parents and suitability of potential cross combinations.…”

Section: Introductionmentioning

confidence: 99%

“…Hybrid seed production is often low in H. macrophylla, possibly due to inbreeding in the cultivated gene pool (Wu and Alexander, 2019). Jones et al (2007) reported from 0 to 20 seeds/ capsule while other researchers have reported as little as 0-5 seeds/ capsule resulting from crosses among H. macrophylla cultivars (Venturieri et al, 2017). Visualization of pollen germination and pollen tube growth allows for the differentiation between pre and post zygotic reproductive barriers which in turn influences the choice of plant breeding techniques (somatic hybridization or embryo culture) that may be used to mitigate incompatibility (Atlagić et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Ploidy Level Influences Pollen Tube Growth and Seed Viability in Interploidy Crosses of Hydrangea macrophylla

Alexander

2020

Front. Plant Sci.

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All Hydrangea macrophylla cultivars tested to date are diploid or triploid and triploid H. macrophylla have thicker stems, larger flowers, and larger stoma compared to related diploids. It is unknown whether interploidy crosses between diploid and triploid hydrangeas can be used to develop triploid varieties. The objective of this study was to compare pollen tube development, fruit formation, and seed viability among intra-and interploidy pollinations of H. macrophylla and evaluate the genome size and pollen viability of resultant progeny. By 24 h post-pollination, pollen tubes had reached the ovaries of diploid flowers in 48.7% of samples while pollen tubes reached the ovaries in only 8.7% of triploid flowers (c 2 = 30.6, p < 0.001). By 48 h post-pollination pollen tubes reached the ovaries of diploid and triploid flowers in 72.5% and 53.8% of samples, respectively (c 2 = 26.5, p = 0.001). There was no difference in percentage of flowers with pollen tubes reaching the ovaries in diploid and triploid flowers at 72 h after pollination (c 2 = 7.5, p = 0.60). Analysis of covariance showed that pollen tube length at 24 and 48 h postpollination was significantly influenced by ploidy and flower length of the female parent. Progeny of interploidy crosses was diploid and aneuploid; no triploid progeny were recovered from crosses using triploid parents. Mean genome sizes of offspring from each cross type ranged from 4.56 pg for 2x × 2x offspring to 5.17 pg for 3x × 3x offspring. Estimated ploidy of offspring ranged from 2x for 2x × 2x crosses to 2.4x for 3x × 3x crosses. Pollen stainability rates of flowering offspring using a modified Alexander's stain ranged from 69.6% to 76.4%.

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Section: Genome Size and Pollen Viability Of Seedlingsmentioning

confidence: 86%

Section: Seed Set Germination and Seedling Growthmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Ploidy Level Influences Pollen Tube Growth and Seed Viability in Interploidy Crosses of Hydrangea macrophylla

Alexander

2020

Front. Plant Sci.

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Oakleaf Hydrangea (HydrangeaquercifoliaBartr.)

Sherwood

Clark

Hokanson

2021

Horticultural Reviews

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Volume-based pollen size analysis: an advanced method to assess somatic and gametophytic ploidy in flowering plants

et al. 2013

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Pollen size is often used as a biological parameter to estimate the ploidy and viability of mature pollen grains. In general, pollen size quantification is performed one- or two-dimensionally using image-based diameter measurements. As these approaches are elaborate and time consuming, alternative approaches that enable a quick, reliable analysis of pollen size are highly relevant for plant research. In this study, we present the volume-based particle size analysis technique as an alternative method to characterize mature pollen. Based on a comparative assay using different plant species (including tomato, oilseed rape, kiwifruit, clover, among others), we found that volume-based pollen size measurements are not biased by the pollen shape or position and substantially reduce non-biological variation, allowing a more accurate determination of the actual pollen size. As such, volume-based particle size techniques have a strong discriminative power in detecting pollen size differences caused by alterations in the gametophytic ploidy level and therefore allow for a quick and reliable estimation of the somatic ploidy level. Based on observations in Arabidopsis thaliana gametophytic mutants and differentially reproducing Boechera polyantha lines, we additionally found that volume-based pollen size analysis provides quantitative and qualitative data about alterations in male sporogenesis, including aneuploid and diploid gamete formation. Volume-based pollen size analysis therefore not only provides a quick and easy methodology to determine the somatic ploidy level of flowering plants, but can also be used to determine the mode of reproduction and to quantify the level of diplogamete formation.

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Analysis of Ploidy Level and Its Effects on Guard Cell Length, Pollen Diameter, and Fertility in Hydrangea macrophylla

Cited by 33 publications

References 10 publications

Ploidy Level Influences Pollen Tube Growth and Seed Viability in Interploidy Crosses of Hydrangea macrophylla

Ploidy Level Influences Pollen Tube Growth and Seed Viability in Interploidy Crosses of Hydrangea macrophylla

Oakleaf Hydrangea (HydrangeaquercifoliaBartr.)

Volume-based pollen size analysis: an advanced method to assess somatic and gametophytic ploidy in flowering plants

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