Production and Surface Modification of Polylactide-Based Polymeric Scaffolds for Soft-Tissue Engineering

Cao, Yang; Croll, Tristan I.; Cooper‐White, Justin; O’Connor, Andrea J.; Stevens, Geoffrey W.

doi:10.1385/1-59259-428-x:87

Cited by 33 publications

(42 citation statements)

References 90 publications

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“…Porous customized scaffolds were cut from 3D PLGA tubes manufactured using the TIPS technique (12 mm diameter and 1-2 mm in width) [13]. The scaffolds were coated with laminin to improve the efficiency of cellular adhesion.…”

Section: Resultsmentioning

confidence: 99%

“…The procedures for fabrication of 3D poly-(lactic-co-glycolic acid) (PLGA) scaffolds using the thermal-induced phase separation (TIPS) technique has been detailed elsewhere and are only described here briefly [13]. 5% (w/v) PLGA was dissolved in 1,4-dioxane at 45°C for 2 h to make a homogenous solution.…”

Section: Poly-(lactic-co-glycolic Acid) Scaffold Manufacturementioning

confidence: 99%

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Transplantation of 3D Scaffolds Seeded with Human Embryonic Stem Cells: Biological Features of Surrogate Tissue and Teratoma-Forming Potential

et al. 2007

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Aim:To generate complex surrogate tissue by transplanting 3D scaffolds seeded with human embryonic stem cells (hESCs) between the liver lobules of severe combined immunodeficient (SCID) mice and to assess the teratoma-forming potential. Materials & methods: 3D poly-(lactic-co-glycolic acid) (PLGA) scaffolds coated with laminin were seeded with hESCs and then transplanted between the liver lobules of SCID mice. After a period of in vivo differentiation, the scaffolds were retrieved and analyzed using reverse transcription polymerase chain reaction, immunofluorescent staining and scanning electron microscopy. Results: A proportion of the hESCs within the scaffolds differentiated into cells that produced proteins characteristic of specific tissues, including endoderm and pancreatic markers glucogon-like peptide-1 receptor, islet amyloid polypeptide and Insulin. Markers of hepatic and neuronal lineages were also investigated. Major matrix proteins abundant in multiple tissue types, including collagen I, laminin and collagen IV, were found to be profuse within the scaffold pores. Transplantation of the seeded scaffolds between liver lobules also resulted in extensive vascularization both from host blood vessel incursion and the differentiation of hESCs into endothelial progenitor cells. An investigation of teratomaforming potential demonstrated that transplantation of 3D scaffolds seeded with hESCs will, under certain conditions, lead to the growth of teratomas. Discussion: Transplantation of 3D scaffolds seeded with hESCs between liver lobules resulted in the development of surrogate tissue containing cells that produced proteins representing the pancreatic, hepatic and neuronal lineages, the assembly of an extracellular matrix structure and the formation of a vasculature. hESCs seeded within 3D scaffolds and transplanted into SCID mice were capable of forming teratomas. However, the formation and progression of teratoma growth is shown to be dependant on both the site of transplantation and the treatment of cells prior to transplantation.

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Section: Resultsmentioning

confidence: 99%

Section: Poly-(lactic-co-glycolic Acid) Scaffold Manufacturementioning

confidence: 99%

Transplantation of 3D Scaffolds Seeded with Human Embryonic Stem Cells: Biological Features of Surrogate Tissue and Teratoma-Forming Potential

et al. 2007

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“…Interface 12: 20150254 secondary amine groups using aminolysis, on PLGA surfaces [156] ( figure 3). For the hydrolysis process, films were immersed in a suitable concentration of aqueous sodium hydroxide (NaOH) for a desired period of time [157][158][159]. For aminolysis, films were immersed in various concentrations of ethylenediamine (ED) or N-aminoethyl-1,3-propanediamine (AEPDA) in either water or isopropyl alcohol for a desired period of time [156,160,161].…”

Section: Chemical Methodsmentioning

confidence: 99%

Strategies for the chemical and biological functionalization of scaffolds for cardiac tissue engineering: a review

Tallawi

Rosellini

Barbani

et al. 2015

J. R. Soc. Interface.

291

208

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The development of biomaterials for cardiac tissue engineering (CTE) is challenging, primarily owing to the requirement of achieving a surface with favourable characteristics that enhances cell attachment and maturation. The biomaterial surface plays a crucial role as it forms the interface between the scaffold (or cardiac patch) and the cells. In the field of CTE, synthetic polymers ( polyglycerol sebacate, polyethylene glycol, polyglycolic acid, poly-L-lactide, polyvinyl alcohol, polycaprolactone, polyurethanes and poly(N-isopropylacrylamide)) have been proven to exhibit suitable biodegradable and mechanical properties. Despite the fact that they show the required biocompatible behaviour, most synthetic polymers exhibit poor cell attachment capability. These synthetic polymers are mostly hydrophobic and lack cell recognition sites, limiting their application. Therefore, biofunctionalization of these biomaterials to enhance cell attachment and cell material interaction is being widely investigated. There are numerous approaches for functionalizing a material, which can be classified as mechanical, physical, chemical and biological. In this review, recent studies reported in the literature to functionalize scaffolds in the context of CTE, are discussed. Surface, morphological, chemical and biological modifications are introduced and the results of novel promising strategies and techniques are discussed.

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“…When, however, the solvent crystallization temperature is higher than that of the liquid-liquid phase separation process, system separation occurs by lowering the temperature and is then known as solid-liquid phase separation. 36,37 Maquet et al describe polymeric composite scaffolds produced by thermal-phase separation using the polymers poly-D,Llactide and poly(lactide-co-glycolide) (PLGA) with Bioglass R as the composite material, which improved the mechanical properties of the scaffold, making it suitable for load-bearing applications. 38 Porosity of scaffold formed by thermal-phase separation technique may be as high as 90% with an average pore diameter of 70 µm.…”

Section: Phase Separationmentioning

confidence: 99%

An Appraisal of the Efficacy and Effectiveness of Nanoscaffolds Developed by Different Techniques for Bone Tissue Engineering Applications: Electrospinning A Paradigm Shift

Sakina

Ali

2014

Adv Polym Technol

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ABSTRACT:The electrospinning method for scaffold production has emerged as a technique of great versatility within the field of tissue engineering. It is a relatively simple technique that enables scaffolds to be manufactured of the required specifications without much ado. Bone scaffolds are challenging in their requirements for mechanical strength and degradability. Polymeric materials can be most efficiently tailored to meet these demands for such scaffolds. This review focuses on the existing technologies involved in the fabrication of polymeric fibrous and porous scaffolds in bone tissue engineering and regenerative medicine applications, as well as on the emerging technologies that are more promising. C

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Production and Surface Modification of Polylactide-Based Polymeric Scaffolds for Soft-Tissue Engineering

Cited by 33 publications

References 90 publications

Transplantation of 3D Scaffolds Seeded with Human Embryonic Stem Cells: Biological Features of Surrogate Tissue and Teratoma-Forming Potential

Transplantation of 3D Scaffolds Seeded with Human Embryonic Stem Cells: Biological Features of Surrogate Tissue and Teratoma-Forming Potential

Strategies for the chemical and biological functionalization of scaffolds for cardiac tissue engineering: a review

An Appraisal of the Efficacy and Effectiveness of Nanoscaffolds Developed by Different Techniques for Bone Tissue Engineering Applications: Electrospinning A Paradigm Shift

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