Production and purification of protease from a Bacillus subtilis that can deproteinize crustacean wastes☆

Yang, Jen-Kuo; Shih, Ing-Lung; Tzeng, Yew‐Min; Wang, San-Lang

doi:10.1016/s0141-0229(99)00164-7

Cited by 251 publications

(154 citation statements)

References 18 publications

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“…The R. prolixus trypanolytic factor's biological activity remained stable at room temperature during parasite counting (10 h) or at 7 to 10.5 pH, possibly due to the protein's chemical or physical stability not being affected, thereby conserving its native state. Lytic activity against T. rangeli KP1(-) continued in EGTA and EDTA chelate agent-treated hemolymph; on the other hand, we deduced that the trypanolytic factor did not belong to the metalloprotein family or any lectin requiring divalent cations for carrying out its normal biological activity as described by Ashida et al (1990, Yang et al (2000), Konozy et al (2003), Liu et al (2006).…”

Section: Discussionmentioning

confidence: 82%

Preliminary characterization of a Rhodnius prolixus hemolymph trypanolytic protein, this being a determinant of Trypanosoma rangeli KP1(+) and KP1(-) subpopulations' vectorial ability

Pulido

Pérez

Vallejo

2008

Mem. Inst. Oswaldo Cruz

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Rhodnius prolixus is the main Trypanosoma rangeli vector in several Latin-American countries and is susceptible to infection with KP1(+) strains; however, it presents an invasion-resistant response to KP1(-) strains. The present work has identified a trypanolytic protein against T. rangeli KP1(-) in the R. prolixus hemolymph which was fractioned with ammonium sulfate (following dialysis). The results revealed a protein component which did not depend on divalent cations for its biological function whilst keeping its trypanolytic activity at temperatures ranging from -20ºC to 37ºC, at 7.0 to 10.5 pH. The protein was partially purified by gel filtration chromatography and ionic exchange chromatography. The major component presented a molecular weight of around 79 kDa and an isoelectric point between 4.9 and 6.3 and may be directly related to hemolymph trypanolytic activity against T. rangeli KP1(-) populations.Key words: Rhodnius prolixus -Trypanosoma rangeli KP1(+) -Trypanosoma rangeli KP1(-) -trypanolytic factorvectorial ability -insect immunity Trypanosoma rangeli is a hemoflagellate which is apparently apathogenous for humans, presenting pathogenicity for the invertebrate host. T. rangeli is of great interest due to its presence in mixed infection with Trypanosoma cruzi in vertebrates and invertebrate hosts, thereby interfering with a clear diagnosis of Chagas' disease and the epidemiology of these parasites in different Latin-American countries .T. rangeli vectors become infected by ingesting blood from wild or domestic reservoirs in natural conditions. The parasite develops in the digestive tract and then invades the hemolymph where it is recognized by the vector's immune system; however, some strains may escape host defense mechanisms, reaching the salivary glands where they become infective forms or metacyclic trypomastigotes (D'Alessandro-Bacigalupo & Gore-Saravia 1992). Salivary gland invasion is a necessary step for transmission, being mediated by one or several ligandreceptor interactions (Basseri et al. 2002).Previous work has revealed two phylogenetically different groups in T. rangeli called T. rangeli KP1(-) and T. rangeli KP1(+), these being mainly transmitted by the 16 Rhodnius species described so far (Vallejo et al. 2002(Vallejo et al. , 2007. Specificity between the vector and the parasite strain which it transmits has been pointed out; each Rhodnius species seems to select the T. rangeli subpopulation to which it is susceptible in nature so that it can then be transmitted to the vertebrate host via insect bite . Nevertheless, a close association has been observed between T. rangeli KP1(+) and vectors from the prolixus group (which includes Rhodnius prolixus and Rhodnius neglectus) and between T. rangeli KP1(-) and vectors from the pallescens group (including Rhodnius pallescens, Rhodnius colombiensis and Rhodnius ecuadoriensis) , Urrea et al. 2005. Recent parasite-vector interaction studies have shown a trypanolytic factor in R. prolixus hemolymph impeding the development of T. rangeli KP1(...

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Section: Discussionmentioning

confidence: 82%

Preliminary characterization of a Rhodnius prolixus hemolymph trypanolytic protein, this being a determinant of Trypanosoma rangeli KP1(+) and KP1(-) subpopulations' vectorial ability

Pulido

Pérez

Vallejo

2008

Mem. Inst. Oswaldo Cruz

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“…Chitinases, chitosanases, chitin deacetylases and proteases [16] from natural sources have been isolated and trialed to develop environmentally friendly chitin and chitosan production [17], as has lactic acid fermentation methods [18].…”

Section: Chitin Biochemistry and Productionmentioning

confidence: 99%

A Review of the Applications of Chitin and Its Derivatives in Agriculture to Modify Plant-Microbial Interactions and Improve Crop Yields

2013

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Abstract:In recent decades, a greater knowledge of chitin chemistry, and the increased availability of chitin-containing waste materials from the seafood industry, have led to the testing and development of chitin-containing products for a wide variety of applications in the agriculture industry. A number of modes of action have been proposed for how chitin and its derivatives can improve crop yield. In addition to direct effects on plant nutrition and plant growth stimulation, chitin-derived products have also been shown to be toxic to plant pests and pathogens, induce plant defenses and stimulate the growth and activity of beneficial microbes. A repeating theme of the published studies is that chitin-based treatments augment and amplify the action of beneficial chitinolytic microbes. This article reviews the evidence for claims that chitin-based products can improve crop yields and the current understanding of the modes of action with a focus on plant-microbe interactions.

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“…Ainsi la variation du pH de 5 à 6 entraîne une augmentation de la production de l'enzyme de 1024,6 U (tableau 5). Cette augmentation montre que le pH 6 du milieu figure parmi les conditions optimales de production de la protéase neutre ; le même résultat a été décrit par Yang et al (2000). Un pH proche de la neutralité a été favorable à la production des protéases neutres par Aspergillus oryzae (Fushimi et al, 1999 production des protéases sont différentes de celles de la croissance de la moisissure (Lenoir et al, 1973 ;Mechakra et al, 1999).…”

Section: Effet Du Phunclassified

Optimisation à l’aide d’un plan d’expériences de la production d’une protéase fongique sur milieu à base de déchets agro-industriels

Benkahoul¹,

Belmessikh²,

Boukhalfa³

et al. 2017

Environnement, Ingénierie &Amp; Développement

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The production of neutral protease was carried out by the cultivation of Aspergillus oryzae Ahlburg (Cohen) 1042.72 in submerged fermentation using orange waste resulting from juice processing as a basal medium enriched with agro-industrial wastes. Optimization of the enzyme synthesis was achieved using the statistical method of Plackett-Burman design with N = 8 experimentsand N-1 factors; five real (corn-steep liquor, pH, salt, decommissioned dates, whey) and two errors. The results were modeled using a linear regression of the type: β0 + β1X1 + β2X2 + β3X3 + β4X4 + β5X5 + e The statistical results (correlations and significance level) made it possible to select the factors allowing the maximum production of protease (2016 U) obtained on a medium containing orange waste enriched in corn-steep liquor in 72 h and at pH 6. A large production is therefore obtained on a cheap medium. La production d’une protéase neutre a été réalisée par fermentation d’une moisissure mésophyle (Aspergillus oryzae Ahlburg (Cohen)1042.72) en erlenmeyers sur milieux à base de déchets d’oranges enrichis à l’aide de sous-produits agro-industriels. L’optimisation de la synthèse de l’enzyme a été effectuée en utilisant une méthode statistique de planification expérimentale (les matrices de Plackett et Burman à N = 8, soit à 8 expériences et N-1 = 7 variables). Les variables utilisées sont les 5 facteurs de production (lactosérum, déchets de dattes ou dattes déclassées, corn-steep liquor, sels minéraux et pH) et 2 erreurs. Les résultats ont été modélisés selon une régression linéaire multiple de type : β0 + β1X1 + β2X2 + β3X3 + β4X4 + β5X5 + e Les résultats statistiques (corrélation et seuil de signification) ont permis de sélectionner les facteurs permettant la meilleure production de l’enzyme. Celle-ci (2016 U) est obtenue sur un milieu à base de déchets d’oranges enrichis en corn-steep liquor après 72 h de fermentation à pH 6. Ainsi, le milieu optimal permet une production importante de protéase neutre sur un milieu à moindre coût.

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Production and purification of protease from a Bacillus subtilis that can deproteinize crustacean wastes☆

Cited by 251 publications

References 18 publications

Preliminary characterization of a Rhodnius prolixus hemolymph trypanolytic protein, this being a determinant of Trypanosoma rangeli KP1(+) and KP1(-) subpopulations' vectorial ability

Preliminary characterization of a Rhodnius prolixus hemolymph trypanolytic protein, this being a determinant of Trypanosoma rangeli KP1(+) and KP1(-) subpopulations' vectorial ability

A Review of the Applications of Chitin and Its Derivatives in Agriculture to Modify Plant-Microbial Interactions and Improve Crop Yields

Optimisation à l’aide d’un plan d’expériences de la production d’une protéase fongique sur milieu à base de déchets agro-industriels

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