Production and purification of extracellular chitinases from Penicillium aculeatum NRRL 2129 under solid-state fermentation

Pusztahelyi, Tünde; Nagy, Viviána; Sandhya, Chandran; Szakács, George; Pócsi, István; Pandey, Ashok

doi:10.1016/j.enzmictec.2004.12.031

Cited by 48 publications

(29 citation statements)

References 29 publications

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“…S1-13 was 1.8 U/g IDS on 11 days incubation (Nopakarn et al 2002). In case of CWB, maximum chitinase activity reported by SSF was 246.6 U/g IDS by B. bassiana BTMF S10 on wheat bran supplemented with colloidal chitin (Suresh and Chandrasekaran 1999), from B. felina RD 101 was 6.34 U/g IDS at 6 days (Pankaj et al 2005a), from P. chrysogenum was 3809 U/g IDS (Pankaj et al 2005b), from P. aculeatum NRRL 2129 was at 72 h (Binod et al 2005) and from T. harzianum was 3.18 at 96 h (Nampoothiri et al 2004). The differences in chitinase yields are related to fermenter design, specifically aeration and humidification suitable for growth (Yoyi et al 2004) and also the difference in the procedures and substrate used for chitinase assays.…”

Section: Resultsmentioning

confidence: 99%

Solid state fermentation production of chitin deacetylase by Colletotrichum lindemuthianum ATCC 56676 using different substrates

2011

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Production of extracellular chitin deacetylase by Colletotrichum lindemuthianum ATCC 56676 under solid substrate fermentation was studied. The suitability of shrimp shell chitin waste (SSCW) and commercial wheat bran (CWB) was evaluated for maximal enzyme production. CWB medium (pH 6.4±0.2) supplemented with chitosan favoured maximal chitin deacetylase yield of 460.4±14.7 unit/g initial dry substrate (U/g IDS) at 96 h as compared to maximal yield of 392.0±6.4 U/g IDS at 192 h in SSCW medium (pH 8.7±0.2) at 25°C incubation temperature and 60% (w/w) initial moisture content of medium. Along with chitin deacetylase, C. lindemuthianum ATCC 56676 produced maximum endo-chitinase (0.28± 0.03 U/g IDS at 144 h) and β-N-acetylhexosaminidase (0.79±0.009 U/g IDS at 192 h) in CWB medium and 0.49± 0.05 U/g IDS of endo-chitinase at 264 h and 0.38±0.04 U/g IDS of β-N-acetylhexosaminidase at 96 h of incubation in SSCW medium. SEM studies indicated the difference in the morphology of mycelia and hyphae of C. lindemuthianum ATCC 56676 when grown on different solid substrates. Production of chitin deacetylase by SSF is being reported for the first time.

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Section: Resultsmentioning

confidence: 99%

Solid state fermentation production of chitin deacetylase by Colletotrichum lindemuthianum ATCC 56676 using different substrates

2011

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“…Some other methods, such as cell immobilization, biphasic cell systems, and solid-state fermentations have been used for improving chitinase production from different microorganisms. Also, process conditions of the culture and its optimization can determine an increase in the productive performance of the microorganisms (Pandey et al 2000;Liu et al 2003;Brand et al 2004;Matsumoto et al 2004;Nampoothiri et al 2004;Binod et al 2005;Dahiya et al 2005b;Nawani and Kapadnis 2005;Patidar et al 2005;Gohel et al 2006).…”

Section: Process Biotechnology and Molecular Biology Applications Tomentioning

confidence: 96%

Fungal chitinases and their biological role in the antagonism onto nematode eggs. A review

Gortari

Hours

2008

Mycol Progress

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Chitin, the most abundant aminopolysaccharide in nature, is a rigid and resistant structural component that contributes to the mechanical strength of chitin-containing organisms. Chemically, it is a linear cationic heteropolysaccharide composed of N-acetyl-D-glucosamine and Dglucosamine units. The enzymatic degradation of chitin is performed by a chitinolytic system with synergistic and consecutive action. Diverse organisms (containing chitin or not) produce a great variety of chitinolytic enzymes with different specificities and catalytic properties. Their physiological roles involve nutrition, parasitism, chitin recycling, morphogenesis, and/or defense. Microorganisms, as the main environmental chitin degraders, constitute a very important natural source of chitinolytic enzymes. Nowadays, the most used method for pest and plant diseases control is the utilization of chemical agents, causative of significant environmental pollution. Social concern has generated the search for alternative control systems (i.e., biological control), which contribute to the generation of sustainable agricultural development. Interactions among the different organisms are the natural bases of biological control. Interest in chitinolytic enzymes in the field of biological control has arisen due to their possible involvement in antagonistic activity against pathogenic chitin-containing organisms. The absence of chitin in plants and vertebrate animals allows the consideration of safe and selective "target" molecules for control of chitin-containing pathogenic organisms. Fungi show appropriate characteristics as potential biological control agents of insects, fungi, and nematodes due to the production of fungal enzymes with antagonistic action. The antagonistic interactions between fungi and plant nematode parasites are among the most studied experimental models because of the high economic relevance. Fungi which target nematodes are known as nematophagous fungi. The nematode egg is the only structural element where the presence of chitin has been demonstrated. In spite of being one of the most resistant biological structures, eggs are susceptible to being attacked by egg-parasitic fungi. A combination of physical and chemical phenomena result in their complete destruction. The contribution of fungal chitinases to the in vitro rupture of the eggshell confirms their role as a pathogenic factor. Chitinases have been produced by traditional fermentation methods, which have been improved by optimizing the culture conditions for industrial processes. Although wild-type microorganisms constitute an alternative source of chitinolytic enzymes, the advances in molecular biology are allowing the genetic transformation of fungi to obtain strains with high capability as biocontrol agents. Simultaneously, a better understanding of rhizosphere interactions, additional to the discovery of new molecular biology tools, will allow the choosing of better alternatives for the biological control of nematodes in order to achieve an integrated management of t...

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“…Esta capacidad quitinolítica fue además estudiada por Binoda, en catorce cepas de Penicillium sp usando un medio mixto con salvado de trigo y quitina para la producción de quitinasa extracelular. La mejor cepa productora de quitinasas fue Penicillium aculeatum NRRL 2129 (ATCC 10409), con un tiempo de producción de 72 h. (26,27) Análisis fisicoquímico de las muestras de suelo, en relación con los hongos aislados Las 3 muestras de cada finca (El Cerezo, Tapias Negras y Sibaté) fueron sometidas a valoración fisicoquímica (resultados no mostrados). Los análi-sis fueron realizados en el laboratorio de suelos de la facultad de Ciencias Agrarias de la Universidad Nacional de Colombia.…”

Section: Actividad Quitinolíticaunclassified

Evaluación de la actividad celulolítica y quitinolítica de hongos filamentosos aislados de rizósfera de cultivos de papa para control de rhizoctonia solani

Valderrama

2017

nova

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45Campuzano et al. Evaluación de la actividad celulolítica y quitinolítica de hongos filamentosos aislados de rizósfera de cultivos de papa para control de rhizoctonia solani Evaluación de la actividad celulolítica y quitinolítica de hongos filamentosos aislados de rizósfera de cultivos de papa para control de rhizoctonia solani Evaluation of the cellulolytic and chitinolytic activity of rhizosphere isolated filamentous fungi from potato cultures to control rhizoctonia solani ResumenObjetivo. Evaluar la actividad celulolítica y quitinolítica de los hongos aislados de rizósfera de papa con posibilidades de ser usados para el control biológico de R. solani. Método. Se aislaron y clasificaron los hongos, mediante sus características macroscópicas y microscópicas. Se aplicaron pruebas para evaluar la capacidad celulolítica y quitinolítica de los hongos y se probó su capacidad antagónica frente al fitopatógeno. Se realizó análisis fisicoquímico del suelo del cual se aislaron los hongos de estudio. Resultado. Del total de hongos caracterizados, solamente una cepa de Rhizopus sp determinada como TN8 fue la que presentó la mejor actividad antagónica frente a R. solani, lo cual, permite considerar este hongo como posible agente de biocontrol para el fitopatógeno de estudio. Palabras claves:Control biológico, hongos celulíticos, hongos quitinolíticos, papa, plaga. AbstractObjective. To evaluate the cellulolytic and chitinolytic activity of fungi isolated from potato rhizosphere with possibilities of being used for the biological control of R. solani. Method. The fungi were isolated and classified by their macroscopic and microscopic characteristics. Tests were applied to evaluate the cellulolytic and chitinolytic capabilities of fungi and their antagonistic capabilities against the phytopathogen were tested. Physicochemical analysis of the soil from which the fungi were isolated were also performed. Result. From the total characterized fungi, only one strain of Rhizopus sp was determined as TN8 and this was the only one that presented the best antagonistic activity against R. solani. This result lead us to consider that this fungus is a possible biocontrol agent for the phytopathogen of this study.

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Production and purification of extracellular chitinases from Penicillium aculeatum NRRL 2129 under solid-state fermentation

Cited by 48 publications

References 29 publications

Solid state fermentation production of chitin deacetylase by Colletotrichum lindemuthianum ATCC 56676 using different substrates

Solid state fermentation production of chitin deacetylase by Colletotrichum lindemuthianum ATCC 56676 using different substrates

Fungal chitinases and their biological role in the antagonism onto nematode eggs. A review

Evaluación de la actividad celulolítica y quitinolítica de hongos filamentosos aislados de rizósfera de cultivos de papa para control de rhizoctonia solani

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