1972
DOI: 10.1128/jb.112.1.235-242.1972
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Production and Purification of a Staphylococcus epidermidis Bacteriocin

Abstract: Liquid cultures of Staphylococcus epidermidis 1580 contained rather small amounts of a bacteriocin, staphylococcin 1580, which was found both in the supernatant fluid and in the cell pellet. It could be extracted from the cells with 5% NaCl solution. The staphylococcin production could not be induced by ultraviolet irradiation or treatment with mitomycin C. Bacteria grown on semisolid medium produced a much larger amount of the compound with a high specific activity. The staphylococcin … Show more

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Cited by 56 publications
(38 citation statements)
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“…In order to produce enhanced quantities of Bac201, procedure of TOR- REBLANCA et al (1989) was followed. The induction was also tried with various concentrations of mitomycin C (0.1, 0.5 and 1.0 Pg/ml) (JETTEN et al 1972), 0.5% mannitol and 2% yeast extract in separate attempts (TAGG et al 1976). Cell bound bacteriocin was eluted by using NaCl (0.5, 1.0, 2.5, 5.0 M), and 0.5 M EDTA (MURIANA and KLAENHAMMER 1991); SDS (0.05, 0.1 and 1.0%) (JETTEN et al 1972) under appropriate conditions.…”
Section: Induction and Elutionmentioning
confidence: 99%
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“…In order to produce enhanced quantities of Bac201, procedure of TOR- REBLANCA et al (1989) was followed. The induction was also tried with various concentrations of mitomycin C (0.1, 0.5 and 1.0 Pg/ml) (JETTEN et al 1972), 0.5% mannitol and 2% yeast extract in separate attempts (TAGG et al 1976). Cell bound bacteriocin was eluted by using NaCl (0.5, 1.0, 2.5, 5.0 M), and 0.5 M EDTA (MURIANA and KLAENHAMMER 1991); SDS (0.05, 0.1 and 1.0%) (JETTEN et al 1972) under appropriate conditions.…”
Section: Induction and Elutionmentioning
confidence: 99%
“…The induction was also tried with various concentrations of mitomycin C (0.1, 0.5 and 1.0 Pg/ml) (JETTEN et al 1972), 0.5% mannitol and 2% yeast extract in separate attempts (TAGG et al 1976). Cell bound bacteriocin was eluted by using NaCl (0.5, 1.0, 2.5, 5.0 M), and 0.5 M EDTA (MURIANA and KLAENHAMMER 1991); SDS (0.05, 0.1 and 1.0%) (JETTEN et al 1972) under appropriate conditions. Preliminary purification of Bac201: Bac201 was precipitated from culture supernatant (obtained by centrifuging overnight growth culture of Staphylococcus aureus AB201 at 8000 u g for 15 min at 4 ºC), by slowly adding ammonium sulfate (with constant stirring at 4 qC) to obtain 50% saturation.…”
Section: Induction and Elutionmentioning
confidence: 99%
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