Production and partial characterization of extracellular proteinases from Streptomyces malaysiensis, isolated from a Brazilian cerrado soil

Nascimento, Rodrigo Pires do; d’Avila-Levy, Claudia M.; Souza, Renan; Branquinha, Marta H.; Bon, Elba P. S.; Pereira-Jr, Nei; Coelho, R. R. R.

doi:10.1007/s00203-005-0030-3

Cited by 9 publications

(4 citation statements)

References 13 publications

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“…For an optimal enzyme production, it is also necessary to reach a good mix of the culture broth because agitation produces a dispersion of air in the culture medium, homogenizes the temperature and the pH, and improves transference rate of nutrients (Markl & Bronnenmeier, 1985). In this respect, similar results were reported by Nascimento et al (2005) for S. malaysiensis AMT-3;De Azeredo et al (2006a, b) for Streptomyces sp. 594; Ramesh et al (2009) for S. fungicidicus MML 1614 and Dixit & Pant (2000) for Nocardiopsis sp.…”

Section: Effect Of Aeration On Alkaline Protease Productionsupporting

confidence: 90%

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Production and Optimization of Alkaline Protease in Submerged Fermentation by Streptomyces rochei NRC 24

2012

Egypt. J. Microbiol.

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Streptomyces rochei NRC 24, under submerged fermentation (SF) conditions. The isolate exhibited maximum enzyme production with fermentation medium composed of peptone, glucose, K 2 HPO 4 , MgSO 4 .7H 2 O, NaCl, and CaCl 2. The studies on environmental parameters revealed that maximum level of alkaline protease production (220.92 U/ml) was achieved during stationary phase of S. rochei NRC 24 with optimum initial pH 9, inoculum size of 2% from stock spore suspension (5.6 x 10 6 spores/ml), and with a culture volume of 75 ml in 250 ml Erlenmeyer flasks under shaking conditions of 200 rpm. Of different nitrogen sources, casein and peptone with 0.4% of each proved to achieve maximum level of enzyme production. Screening experiments on medium components showed that the presence of peptone, glucose, MgSO 4 .7H 2 O, NaCl and CaCl 2 were essential for alkaline protease production. The absence of K 2 HPO 4 from the medium did not totally influence the enzyme production. Based on these results, De Meo's fractional factorial design was applied to find out the optimal conditions for maximal alkaline protease production. Optimization of the medium was carried out in three steps and Streptomyces rochei NRC 24 grew in the optimized medium producing 555.61 U/ ml compared to the growth of the strain on the basal medium before optimization (224 U/ ml).

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Section: Effect Of Aeration On Alkaline Protease Productionsupporting

confidence: 90%

“…Peptone, 4; Glucose, 10; K2HPO4, 0.5; MgSO4.7H2O, 0.5; NaCl, 0.5; CaCl2, 0.5; Distilled water, 1000 ml; pH, 9 (Ramesh et al, 2009). 5 KH2PO4, 1.5; K2HPO4 , 9.0; MgSO4.7H2O, 0.2; CaCl2, 0.05; MnSO4, 0.01; ZnSO4.7H2O, 0.001;Yeast extract , 0.01; Wheat bran, 0.25; Distilled water, 1000 ml; pH, 9 (Nascimento et al, 2005).…”

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confidence: 99%

Production and Optimization of Alkaline Protease in Submerged Fermentation by Streptomyces rochei NRC 24

2012

Egypt. J. Microbiol.

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“…da Silva (2012) described 2152 complete or partial isolates of actinobacteria from Cerrado soil samples that differed phenotypically from previously described members of this phylum. Some actinobacteria isolated from Cerrado soil samples exhibit chitinolytic and proteinase activities (Petinate et al 1997;Gomes et al 2000;Nascimento et al 2005). Other studies have also described the isolation of novel actinomycetes and acidobacterial species from Cerrado soil (Albuquerque de Barros et al 2003;.…”

Section: Microorganisms In Ecosystem Function: Taxa Vs Functionmentioning

confidence: 94%

Diversity and Function of Bacterial Assemblages in Savanna Vegetation Soils

Catão

Lopes

Silva

et al. 2014

Sustainable Development and Biodiversity

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Savannas can be found in North and South America, Africa, Asia, and Oceania. The Cerrado is a vast savanna located mainly in the central region of Brazil. Although, Cerrado ecosystems are similar in vegetation structure, differences in soil characteristics influence the microbiota. Throughout the world savannas are rapidly being converted to agricultural and urban uses, altering physical and chemical properties of the soil, as well as microbial diversity through changes in bacterial and fungal richness, community structure, and activity. The studies addressed in this review describe soil microbial communities present in Cerrado ecosystems, which are dominated by Acidobacteria, Proteobacteria, and Actinobacteria. We highlight the importance of microbial communities to ecosystem services such as nutrient cycling, regulation of biogeochemical processes, and contribution to net primary production. Sustainable development based on the use of natural resources requires a better understanding of the microbial processes and genetic resources in this biome.

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“…Previous studies have reported high numbers of Actinobacteria in Cerrado soils (Nascimento et al, 2005;Suela Silva et al, 2013; de Sales et al, 2017;Vieira et al, 2018;Vargas Hoyos et al, 2019). These soils are acidic and have a low availability of macronutrients (nitrogen, P, potassium, calcium, magnesium, and sulfur) and micronutrients (boron, copper, molybdenum, and zinc) (Cruz Ruggiero et al, 2002;Oliveira and Marquis, 2002;Genuário et al, 2019).…”

Section: Potential Of Phosphorus Mobilizationmentioning

confidence: 99%

An Actinobacterium Strain From Soil of Cerrado Promotes Phosphorus Solubilization and Plant Growth in Soybean Plants

Hoyos

Chiaramonte

Barbosa-Casteliani

et al. 2021

Front. Bioeng. Biotechnol.

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The huge biological diversity of the Brazilian Cerrado is an important source of economically interesting microbial agents. The phylum Actinobacteria plays an important role in nutrient cycling, potentially improving their availability to plants. In this study, we isolated an actinobacteria (strain 3AS4) from wheat rhizospheres of crops cultivated in the Cerrado biome. Strain 3AS4 was identified as belonging to the genus Streptomyces and had phosphorus mobilization ability, mineralizing approximately 410 μg ml–1 from phytate, 300 μg ml–1 from calcium phosphate, and 200 μg ml–1 from rock phosphate. The analysis of the actinobacteria crude extract by spectrometric techniques revealed the presence of gluconic and 2-ketogluconic acid, and a greenhouse experiment was carried out to evaluate its plant growth promotion activity in soybean. Soil in its natural condition (with no phosphorus addition), 40 kg ha–1 rock phosphate from Bayovar (RP) added to soil, and triple super phosphate (SPT) added to soil were used. Significant differences in plant height were observed at 6 weeks when the plants were inoculated with the 3AS4 strain. The growth of inoculated plants in natural condition was promoted in 17% compared with the RP and SPT non-inoculated conditions, suggesting that inoculation can enable plants to grow with lower chemical P fertilizers. In the plants that were inoculated with the 3AS4 strain in the RP condition, the plant height increased by approximately 80% and the shoot:root ratio was approximately 30% higher compared to control conditions (non-inoculated plants in natural conditions). 3AS4 has P-solubilizing potential and can be exploited as an inoculant for soybean cultivation. These results suggest that this actinobacterium is a valuable resource for sustainable agriculture and will allow the reduction of phosphate fertilization in the future.

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Production and partial characterization of extracellular proteinases from Streptomyces malaysiensis, isolated from a Brazilian cerrado soil

Cited by 9 publications

References 13 publications

Production and Optimization of Alkaline Protease in Submerged Fermentation by Streptomyces rochei NRC 24

Production and Optimization of Alkaline Protease in Submerged Fermentation by Streptomyces rochei NRC 24

Diversity and Function of Bacterial Assemblages in Savanna Vegetation Soils

An Actinobacterium Strain From Soil of Cerrado Promotes Phosphorus Solubilization and Plant Growth in Soybean Plants

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