Production and partial characterization of proteases produced by Bacillus licheniformis isolated from cow rumen ingesta

Abstract

Background: In recent decades, proteases have gained a lot of recognition because of their applications in various industries, such industries as food, leather, pharmaceutical industries etc. The use of cheap substrates such as agro-industrial wastes could help in meeting industrial demand. Method: In this study, we evaluated the ability of groundnut shell and Bean chaff compared to that of glucose as carbon sources to support proteases production by Bacillus licheniformis isolated from cow rumen ingesta. Results: The optimum pH of the proteases produced with all carbon sources was observed at pH 6, while the optimum temperature of proteases produced with Bean chaff, groundnut shell and glucose were 50, 40 and 30 °C, respectively. However, the activities of proteases produced with agro-industrial wastes were significantly higher (p<0.05) than that of glucose under optimum pH and temperature conditions, though protease produced with mango seeds had higher activity. The Km of the proteases produced with groundnut shell, Bean chaff and glucose were 9.78, 3.52 and 1.24 mg/mL while their Vmax were 555.56, 434.78 and 222.22 U/mL, respectively. Conclusion: The lower Km of proteases produced with the agro-industrial wastes suggests that both the carbon sources could be used in place of glucose, though glucose was preferred by the isolate. The presence of Bacillus licheniformis with proteolytic activity in the cow rumen ingesta could mean that the site could be a potential source of proteolytic isolates.

Feed intake, fiber fraction digestibility and milk yield of lactating goats fed banana peel in the diet

“…Banana processing produces 30-40% banana peel [2]. The most dominant proximate component of banana peels is carbohydrates (63.32%) [3]. Banana peels are rich in nutrients such as carbohydrates, amino acids, and minerals that needed by animals.…”