Production and partial characterization of thermophilic proteases from Streptomyces sp. isolated from Brazilian cerrado soil

Azeredo, Luciana A. I. de; Freire, Denise Maria Guimarães; Soares, R. Félix; Leite, Selma Gomes Ferreira; Coelho, R. R. R.

doi:10.1016/j.enzmictec.2003.11.015

Cited by 50 publications

(26 citation statements)

References 18 publications

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“…Shaking at 100 rpm markedly increase proteases production by S. aburaviensis. This finding was also reflected in other studies [24,26,28,29].…”

Section: Discussionsupporting

confidence: 78%

“…The enzymes were stored at 4°C with few drops of toluene/Sodium Azide to avoid bacterial contamination. Protease assay was done by using the method described by Meyers and Ahearn [23], which is a modified method of Hayashi et al [24]. Briefly, 3 ml of crude enzyme, 3 ml of citrate phosphate buffer and 3 ml of 1% (w/v) casein was taken in a 25 ml test tube, and the tube was placed in a water bath at 35°C for 1 hour.…”

Section: Measurement Of Enzyme Activitymentioning

confidence: 99%

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Effects of Cultural Conditions on the Production of Extracellular Protease by Streptomyces albolongus and Streptomyces aburaviensis

N¹

2013

Enz Eng

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Proteolytic activity of two isolates of actinomycetes, Streptomyces albolongus and Streptomyces aburaviences was investigated on the basis of their ability to hydrolyze skimmed milk casein, egg albumin and gelatin. Both isolates were found to have potential for extracellular proteases production. Effects of culture conditions for the production of extracellular protease from S. albolongus and S. aburaviensis were determined. Highest protease yield from S. albolongus was obtained after 5 days of incubation with an initial p H 7, at static state when inoculated in medium composed of 1% glucose, 2% beef extract, 0.2% yeast extract, 0.1% KH 2 PO 4 , 0.3% K 2 HPO 4 , and trace MgSO 4 .7H 2 O. Optimum incubation conditions for S. abureviences were 4 days, with an initial medium p H 8 at shaking condition (100 rpm). S. aburaviences preferred 1.5% lactose and 1.5% tryptone as a carbon and nitrogen source. Both the isolates showed maximum protease yield at 37°C. The result of the present study might be helpful for largescale production of extracellular protease from these Streptomyces spp.

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“…Shaking at 100 rpm markedly increase proteases production by S. aburaviensis. This finding was also reflected in other studies [24,26,28,29].…”

Section: Discussionsupporting

confidence: 78%

Section: Measurement Of Enzyme Activitymentioning

confidence: 99%

Effects of Cultural Conditions on the Production of Extracellular Protease by Streptomyces albolongus and Streptomyces aburaviensis

N¹

2013

Enz Eng

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“…Activity staining was performed on 0.1% copolymerized gelatin as substrate to study protease activity, according to De Azeredo et al (4). Lipase activity staining was performed using tributyrin agar according to the method of Singh et al (22).…”

Section: Characterisation Of Enzymesmentioning

confidence: 99%

Concomitant production of protease and lipase by Bacillus licheniformis VSG1: production, purification and characterization

Sangeetha

Geetha

Arulpandi³

2010

Braz. J. Microbiol.

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This study was aimed at producing protease and lipase simultaneously on a common medium by Bacillus licheniformis VSG1, which was isolated from a tannery effluent. The effect of media composition with respect to protein source, lipid source and emulsifier on the production of protease and lipase was analysed. Both those enzymes were produced under optimized conditions like pH, temperature and incubation time. The enzyme mixture comprising of both protease and lipase was purified by ammonium sulphate precipitation, dialysis and gel filtration chromatography to obtain 20-fold pure enzymes.The purified enzyme mixture was characterized to determine the optimum pH and temperature of protease and lipase, the response of the enzymes to inhibitors, additives and solvents. The molecular weight of both the enzymes was determined as 40 kDa on SDS-PAGE. The concomitant production of protease and lipase and the purification of both the enzymes in a single mixture have industrial significance, as many industrial processes use both protease and lipase together.

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“…Out of the four temperatures tested (40, 50, 60 and 70 o C), 60 o C was found to be the optimal for maximum enzyme activity irrespective of strain. Enzymes with high thermostability are more viable and have a better efficiency (De Azeredo et al, 2004). Activity of these proteases decreased at a temperature of 50 o C. At higher temperature (70 o C), the protease activity was also lower.…”

Section: Shake Flasks Experiments -Growth Of Isolated Strainsmentioning

confidence: 99%

Production of thermostable protease enzyme in wastewater sludge using thermophilic bacterial strains isolated from sludge

Chenel

Tyagi

Surampalli

2008

Water Science and Technology

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Abstract:The volume of sludge produced annually is very high and poses serious disposal problems. The traditional methods of sludge disposal produce secondary pollutants. Therefore, the alternate or suitable solution is reuse of sludge in an ecofriendly approach. Biotechnology is an interesting tool to add value to the processes involved in wastewater and wastewater sludge disposal/reuse. In this context, a study was carried out on thermophilic bacterial strains that produce thermostable proteases. The bacterial strains were first isolated from municipal wastewater sludge. In contrast to the conventional strains used in industries, like Bacillus sp., the new strains were Gram-Negative type. In semi-synthetic medium, a maximal protease activity of 5.25 IU/ml (International Unit per ml) was obtained at a pH of 8.2 and at a temperature of 60 o C, which is higher than the stability temperature of 37°C for a similar protease obtained from the conventional producer Bacillus licheniformis. Moreover, growth and protease activity of the strains were tested in wastewater sludge. It is expected that the complexity of sludge could stimulate/enhance the protease production and their characteristics. In conclusion, reuse of wastewater sludge will help to reduce their quantity as well as the value-added products produced will replace chemical products used in industries.Keywords: Thermophilic strains; thermostable proteases; wastewater sludge INTRODUCTIONThe disposal of wastewater sludge (WWS) generated during the treatment of the various municipal and industrial wastewater (WW) is one of the major problems in safeguarding of the planetary environment. During the last fifty years, people believed that the discharge of WW and WWS in the rivers and the lakes was not detrimental to the environment because the latter had the capacity to self purify and regenerate themselves. However, studies during the last twenty years have contradicted these facts. At the present time, the rivers and the lakes do not have the capacity to absorb all the pollutants which are poured there (Barnabé and et al., 2003). It thus became imperative to find new methods in order to decrease and/or re-use these wastes. Several of these new methods have been developed in recent past and others are being developed.

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Production and partial characterization of thermophilic proteases from Streptomyces sp. isolated from Brazilian cerrado soil

Cited by 50 publications

References 18 publications

Effects of Cultural Conditions on the Production of Extracellular Protease by Streptomyces albolongus and Streptomyces aburaviensis

Effects of Cultural Conditions on the Production of Extracellular Protease by Streptomyces albolongus and Streptomyces aburaviensis

Concomitant production of protease and lipase by Bacillus licheniformis VSG1: production, purification and characterization

Production of thermostable protease enzyme in wastewater sludge using thermophilic bacterial strains isolated from sludge

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