Production and partial characterization of dehairing protease from Bacillus cereus MCM B-326

Purification, characterization and application of novel alkaline protease from new Bacillus cereus UV-15 mutant

“…The effect of the temperature on alkaline protease was determined by incubating the reaction mixture (pH 10.0) for 20 minutes, at different temperatures ranging from 30 to 80ºC (30,40,50, 60, 70 and 80ºC) and measuring the activity with standard proteolytic activity assay. Before the addition of enzymes, the substrate ( casein) was preincubated at the respective temperature for 10 minutes.…”

“…Before the addition of enzymes, the substrate ( casein) was preincubated at the respective temperature for 10 minutes. The thermostability of the crude alkaline protease was investigated by measuring the residual activity after incubating the enzyme at various temperatures ranging from 30 to 60ºC (30,40,50, 60, 70 and 80ºC) for 30 and 60 minutes, respectively.…”

Scale up of production in a bioreactor of a halotolerant protease from moderately halophilic Bacillus sp. isolated from soil

“…in the medium containing 1% casein, inoculated with 1% overnight grown culture (initial pH of the medium 7.0), agitation speed 50 rpm, and 32°C in batch fermentation. Samples were drawn at every six hours interval up to 10 days (Nilegaonkar et al 2007).…”

“…Similar observations were made and discussed by Singh et al (2004) for Bacillus sphaericus secreting maximum proteases activity during postexponential growth phase. A dehairing protease from B. cereus, reached to 126.87 ± 1.32 Uml -1 in 36h of the fermentation when the cell growth reached late log phase or early stationary phase (Nilegaonkar et al 2007). These differences in growth rate between the previous reports and the present findings might be due to differences in cultivation conditions as well as the Bacillus species.…”

Isolation and characterization of a haloalkaliphilic protease producer bacterium from Wadi Natrun in Egypt

“…Though the presence of casein enhances protease production in some Bacillus strains (Belmessikh et al, 2013), it is likely that casein is not among the best protease inducers for the experimental Bacillus strain. Similarly, slight negative effects of casein on enzyme production by other related bacterial strains have been formerly reported (Nilegaonkar et al, 2007;Daroit et al, 2011).…”