Purification, characterization and application of novel alkaline protease from new Bacillus cereus UV-15 mutant

Abstract

The alkaline protease produced by Bacillus cereus

Laundry Detergent Compatibility and Dehairing Efficiency of Alkaline Thermostable Protease Produced from <i>Aspergillus terreus</i> under Solid-state Fermentation

“…The purified alkaline protease of A. terreus was found to have good activity at alkaline value with the maximum at pH 9.5. These results are in agreement with the previous findings on another protease from Serratia marcescens 39 and Bacillus cereus 45 .…”

“…The temperature of the process is a parameter that influences the reaction rates collision and the cellular metabolism 34 . Similar results were obtained by Hossain et al 35 who recorded the same temperature 45 for protease production from A. flavus AP2. Higher incubation temperature was reported by Murthy and Naidu 24 for A. oryzae protease at 60 .…”

“…Our alkaline protease was stable under alkaline conditions between the pH ranges 9-11 thereby; it is suitable for using in leather industry. Similar results reported the potentiality of protease in removing hide hairs 23,45 .…”

“…2.9 Optimization of alkaline protease production parameters The SSF was also optimized through various parameters including moisture content of substrate 58, 71.5, 78.3, 82 and 85 , incubation period 2, 3, 4, 5, 6 and 7 days , incubation temperature 30,35,40,45,50,55 and 60 , size of inoculum 0.5, 1.0, 1.5, 2.0, 2.5, and 3.0 mL of spores suspension and initial pH of basal medium 8.0, 8.5, 9.0, 9.5, 10.0, 10.5 and 11.0 . The effect of supplementation of the basal medium with different by-products whey, cane molasses, beet molasses and corn steep liquor as additives 5 , v/v and effect of different concentrations of whey 5,10,15,20,25, and 30 were also ascertained.…”

Study of Uv Mutated Protease Enzyme From Various Sources

“…The plate was separated into two portions; one half of the portion was covered with aluminum foil that protects microbes from the UV exposure. The plate was placed under UV exposure for different time periods of (10, 20, 30 min) and incubated at 37 ˚C for 24 h, mutant colony were used for future study 14 .…”