Production and partial characterization of multifunctional lipases by Sporobolomyces ruberrimus using soybean meal, rice meal and sugarcane bagasse as substrates

Ferraz, Lenir Rigoli; Oliveira, Daniela dos Santos de; Silva, Marceli Fernandes; Rigo, Elisandra; Luccio, Marco Di; Oliveira, J. Vladimir; Oliveira, Débora de; Treichel, Helen

doi:10.1016/j.bcab.2012.03.008

Cited by 25 publications

(4 citation statements)

References 35 publications

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“…Salgado et al (2014), Toscano et al (2013), Coradi et al (2013) and Ferraz et al (2012) reported the positive impact of lipids in inducing lipase production. In accordance with the literature data, this study reported the highest lipase activity using the waste with the highest lipid content; namely, cottonseed meal (run 3).…”

Section: Enzyme Production Using Formulations Containing Different Prmentioning

confidence: 98%

A multicomponent system based on a blend of agroindustrial wastes for the simultaneous production of industrially applicable enzymes by solid-state fermentation

et al. 2018

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Section: Enzyme Production Using Formulations Containing Different Prmentioning

confidence: 98%

A multicomponent system based on a blend of agroindustrial wastes for the simultaneous production of industrially applicable enzymes by solid-state fermentation

et al. 2018

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“…Moisture higher than 70% led to a decrease in lipase activity, due to a change in the structure of the medium resulting in porosity decrease, which hinders mass transfer and the strain development. Ferraz et al have received similar results and determined that the optimal moisture for lipase production by Sporobolomyces ruberrimus was 60% [18]. Fig.…”

Section: Effect Of Particles Size and Moisture Content On Lipase Biosmentioning

confidence: 68%

Optimization of Lipase Production in Solid-State Fermentation by Rhizopus Arrhizus in Nutrient Medium Containing Agroindustrial Wastes

Dobrev¹,

Strinska²,

Hambarliiska³

et al. 2018

TOBIOTJ

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Background: Rhizopus arrhizus is a potential microorganism for lipase production. Solid-state fermentation is used for microbial biosynthesis of enzymes, due to advantages, such as high productivity, utilization of abundant and low-cost raw materials, and production of enzymes with different catalytic properties. Objective: The objective of the research is optimization of the conditions for lipase production in solid-state fermentation by Rhizopus arrhizus in a nutrient medium, containing agroindustrial wastes. Method: Biosynthesis of lipase in solid-state fermentation by Rhizopus arrhizus was investigated. The effect of different solid substrates, additional carbon and nitrogen source, particles size and moisture content of the medium on enzyme production was studied. Response surface methodology was applied for determination of the optimal values of moisture content and tryptone concentration. A procedure for efficient lipase extraction from the fermented solids was developed. Results: Highest lipase activity was achieved when wheat bran was used as a solid substrate. The addition of 1% (w/w) glucose and 5% (w/w) tryptone to the solid medium significantly increased lipase activity. The structure of the solid medium including particles size and moisture content significantly influenced lipase production. A mathematical model for the effect of moisture content and tryptone concentration on lipase activity was developed. Highest enzyme activity was achieved at 66% moisture and 5% (w/w) tryptone. The addition of the non-ionic surfactant Disponyl NP 3070 in the eluent for enzyme extraction from the fermented solids increased lipase activity about three folds. Conclusion: After optimization of the solid-state fermentation the achieved 1021.80 U/g lipase activity from Rhizopus arrhizus was higher and comparable with the activity of lipases, produced by other fungal strains. The optimization of the conditions and the use of low cost components in solid-state fermentation makes the process economicaly effective for production of lipase from the investigated strain Rhizopus arrhizus.

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“…In addition, the strain was unable to reproduce sexually by producing ellipsoidal teliospores, which is characteristic of S. metaroseus . The species S. ruberrimus was first described by Yamasaki and Fujii in 1950[ 32 ] and found to synthesize carotenoids[ 33 ] and lipases [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

Diversity of Red Yeasts in Various Regions and Environments of Poland and Biotechnological Potential of the Isolated Strains

Kot,

Sęk,

Kieliszek

et al. 2023

Appl Biochem Biotechnol

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Due to the growing demand for natural carotenoids, researchers have been searching for strains that are capable of efficient synthesis of these compounds. This study tested 114 red yeast strains collected from various natural environments and food specimens in Poland. The strains were isolated by their ability to produce red or yellow pigments in rich nutrient media. According to potential industrial significance of the carotenoids, both their total production and share of individual carotenoids (β-carotene, γ-carotene, torulene, and torularhodin) were analyzed. The total content of carotenoid pigments in the yeast dry matter ranged from 13.88 to 406.50 µg/g, and the percentages of individual carotenoids highly varied among the strains. Most of the yeast isolates synthesized torulene at the highest amount. Among the studied strains, isolates with a total carotenoid content in biomass greater than 200 µg/g and those containing more than 60% torularhodin were selected for identification (48 strains). The identified strains belonged to six genera: Rhodotorula, Sporidiobolus, Sporobolomyces, Buckleyzyma, Cystofilobasidium, and Erythrobasidium. The largest number of isolates belonged to Rhodotorula babjevae (18), Rhodotorula mucilaginosa (7), Sporidiobolus pararoseus (4), and Rhodotorula glutinis (4).

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Production and partial characterization of multifunctional lipases by Sporobolomyces ruberrimus using soybean meal, rice meal and sugarcane bagasse as substrates

Cited by 25 publications

References 35 publications

A multicomponent system based on a blend of agroindustrial wastes for the simultaneous production of industrially applicable enzymes by solid-state fermentation

A multicomponent system based on a blend of agroindustrial wastes for the simultaneous production of industrially applicable enzymes by solid-state fermentation

Optimization of Lipase Production in Solid-State Fermentation by Rhizopus Arrhizus in Nutrient Medium Containing Agroindustrial Wastes

Diversity of Red Yeasts in Various Regions and Environments of Poland and Biotechnological Potential of the Isolated Strains

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