Production and localization of human prolactin in the placental and decidual tissue at term.

Immunolocalization of 91 kDa Ascitic Protein in the Human Placenta

“…Deparaffinized sections kept in 0.01 M phosphate-buffered saline (PBS), pH 7.3, were subjected to the following protocol: (1) enzymatic digestion with 0.1% (w/v) pepsin in 0.05 M Tris-HCI buffer, pH 7.6, containing 0.1% (wlv) CaCl2, 30 min at 37°C; (2) 3 washes with PBS, 5 min each; (3) blocking of endogenous peroxidase activity with 5 mM sodium metaperiodate solution, 10 min; (4) 3 washes with PBS, 5 min each; (5) blocking freealdehyde groups with 3 mM sodium borohydride solution, 10 min; (6) 3 washes with PBS, 5 min each; (7) pre-treatment with highly diluted (1:2,500) normal goat serum, 30 min; (8) incubation with the specific antibody diluted at 1:400 to 1:2,000 with PBSA (PBS containing 0.5% bovine serum albumin), 2-411; (9) 3 washes with PBS, 10 min each; (10) incubation with biotin-labelled goat anti-rabbit IgG, 30 min; (11) 3 washes with PBS, 10 min each; (12) incubation with avidin-biotin-HRP complex, 30 min; (13) 3 washes with PBS, 10 min each.…”

Prolactin synthesis and trophoblastic differentiation in metastatic choriocarcinoma in the liver.

“…In fact, though the ultrastructure of the decidual cells demonstrates the secretory granules in the cytoplasm (20), they are possibly concerned not with peptide hormone synthesis but with the production of acid phosphatase (22). Previously the authors described immunohistological evidence to demonstrate that syncytium played a role of PRL synthesis in normal chorionic villi (7,8).…”