Summary : Biotinylated-enzymes affinity cytochemistry using biotinyl-horseradish peroxidase (B-HRP) and -alkaline phosphatase (B-ALPase) was investigated in the laying hen oviduct.Endogenous biotin-binding activities (EBBA) were demonstrated in granules of tubular gland cells and non-ciliated unicellular epithelial cells in both the lower magnum and the isthmus when the ovum descended into the lower isthmus or uterus.Biotin affinity secretory granules were electron dense and inhomogenous (or cored with dense and less dense regions) and the in size varied from large to small in acinar cells and to small in the epithelium, respectively. Utilizing this method, EBBA suppressed oviductal tissue synthesizing endogenous avidin, thus facilitating the interpretation of a specific avidin-biotin reaction system introduced into the various histo-and cytochemical tools.On the other hand, the resultant demonstration of avidin disagrees with ordinal data that had beeh localized in the oviductal goblet cells obtained from chicken oviduct stimulated by ovarian hormones.It is desirable to study the localization of immunoreacting and B-HRP and/or B-ALPase affinity avidin using ovulation occurring in a 24 hr daily period in hens or quails, and/or non-laying ones given gonadal hormones.
The present study examined the tissue-expression of MRP8 in human placenta using a biotinylated DNA-probe for in situ hybridization. During the first and second trimesters high level and synchronous expression of MRP8 was detected in cytotrophoblasts (Langhans' cells), placental-tissue macrophages (Hofbauer cells), fibroblast-like cells, endothelial cells and monocytic lineages in the foetal capillaries. The highest expression was seen in large and oval-shaped cytotrophoblasts and stromal-cell populations at around 8-11 weeks. At term placentas had low level MRP8 expression chiefly in the myelomonocytic lineages in foetal blood vessels. The peripheral monocytes in the maternal space also expressed MRP8 at high levels during the first and second trimesters, which subsequently decreased at term. We suggest three hypotheses based on these results; (1) The initial expression of MRP8 may occur in two cell lineages of extra-embryonic and intra-embryonic origin in the first two trimesters; (2) the cytotrophoblasts, placental-tissue macrophages and fibroblasts may play important roles in the production of placental hormones and the immuno-regulation of foetal acceptance; and (3) MRP8-expression may be synchronously inhibited once the trophoblasts and stromal cell-constituents have differentiated in the chorionic villi.
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