Production and evaluation of leukocyte- and thrombocyte-rich fibrin membranes in birds

Abstract

The aim of this study was the preparation and histological evaluation of Leukocyte- and Thrombocyte-Rich Fibrin (L-TRF) membranes obtained from the blood of four bird species. Forty adult healthy birds were divided into four groups of equal size: G1 – macaws, G2 – domestic chickens, G3 – parrots, G4 – toco toucans. A total of 0.5 mL of blood was collected from each bird, put into a glass tube without anticoagulant and centrifuged at 3000 rpm for 10 min. L-TRF membranes produced after compression of the clot were processed for histological analysis. The ratio of thrombocytes/area was not significantly different among Groups G2, G3 and G4, but a significant difference was found between Groups G1 and G2 with the highest thrombocyte concentration/area in G1. The groups did not differ statistically in the number of leukocytes/area. The fibrin-to-cells ratio did not vary statistically among Groups G1, G2 and G3, but this ratio was significantly higher in Group G4 than in the other groups. The thrombocyte-to-leukocyte ratio was the highest in Group G1, but it did not differ among Groups G2, G3 and G4. In conclusion, the centrifugation protocol allowed the production of L-TRF membranes in the four bird species studied. Histologically, cell ratios were analogous in domestic chickens and parrots, and macaws had the highest ratio of thrombocytes.

Effective treatment of a severe neck wound in a greylag goose (Anser anser) using autologous thrombocyte‐rich plasma and xenogeneic fibrin mesh

“…The benefits of platelet‐rich plasma in wound healing have been well‐documented in mammals, 5 including promoting new tissue formation, reducing inflammation, improving blood flow to the wound site and exerting antimicrobial properties 20 . However, there are few published studies on producing fibrin membranes 21 and thrombocyte‐rich biological products 22 in birds. Therefore, more research is necessary to fully understand the potential of these products in promoting tissue regeneration in avian species, and to optimise the treatment protocol for free‐ranging birds.…”