Production and composition of microbial fat from Rhodotorula glutinis

Abstract

The fatty acid, sterol and hydrocarbon compositions of oil produced by a new strain of Rhodotorula glutinis isolated from soil and grown on molasses, were determined. The major constituent fatty acids were: palmitic (37%) oleic (47%) and linoleic (8%). The major sterols were campesterol (42%) and stigmasterol (27%) and the major hydrocarbons were n-C23 (37%), iso-CZ9 (30%) and n-C2' (19%). Minor constituents in each class of lipids were also detected and estimated. The oil produced was 54% on a dry weight basis. The fatty acid composition was found to be close to that of palm oil.

A soluble diacylglycerol acyltransferase is involved in triacylglycerol biosynthesis in the oleaginous yeast Rhodotorula glutinis

“…The enzyme did not prefer the medium-and short-chain fatty acids. This also supports the fact that major constituents of microbial fat of R. glutinis are oleic (47 %) and palmitic (37 %) (Misra et al, 1984 21 under the standard assay conditions. These data suggest that RgDGAT exhibits broad specificity for acyl-CoAs, with greater preference for oleoyl-CoA.…”

Evaluation of Waste Loquat Kernels as Substrate for Lipid Production by Rhodotorula glutinis SO28

“…The other fatty acids were stearic acid (C18:0) (8.3 %), alpha-linolenic acid (C18:3) (5.9 %) and myristic acid (C14:0) (2.6 %). The fatty acid composition of R.glutinis SO28 was similar to those of other R.glutinis strains reported by previous studies [39][40][41]. The Table 4 Effect of additional phosphorus source on lipid accumulation in R. glutinis Waste Biomass Valor most prominent fatty acid of R. glutinis S028 was oleic acid.…”

Validation of a gas–liquid chromatographic method for analysing samples rich in long chain n−3 polyunsaturated fatty acids: Application to seafood

“…Normally those peaks are related to large amounts of cholesterol and other nonsaponifiable lipids present in the samples (Meier et al, 2006). However, we did not find evidence of any strange peaks or artefacts interfering on FA chromatographic peaks probably because the non-saponifiable components have been discarded previous to the methylation step, as reported in other studies (Misra et al, 1984;Misra et al, 1985;Sahgk and Imre, 1997). TMS-DM has been reported to generate artefacts and impurities that would likely interfere with short-chain FA analysis by GLC (Park et al, 2001), however the importance of short-chain FA in marine tissues is scarce.…”