Validation of a gas–liquid chromatographic method for analysing samples rich in long chain n−3 polyunsaturated fatty acids: Application to seafood

Juárez, M.; Juárez, A.; Aldai, Noelia; Avilés, C.; Polvillo, Oliva

doi:10.1016/j.jfca.2010.03.011

Cited by 13 publications

(16 citation statements)

References 43 publications

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“…a). These values were comparable to previous findings observed in the internal validation of the original method (Juárez et al, ).…”

Section: Resultssupporting

confidence: 92%

“…The former method was employed in the most recent analyses of reared cod larvae individuals (0.07-9 mg, dry weight) (Folkvord et al, 2017) and pooled Baltic herring samples (0.4-0.5 g, wet weght) (Lind et al, 2018). However, the acid-catalyzed method forms artifacts from tissue components during long-time reaction at high temperature (Aldai et al, 2005(Aldai et al, , 2006 García, 2000;Juárez et al, 2010). In fact, we observed many GC peaks interfering with C22-24 fatty acids, when methyl esters were prepared from juvenile tuna muscle by heating it in 2 M HCl-methanol at 90 C for 2 h (chromatogram not shown).…”

Section: Validation Of Gc Analysismentioning

confidence: 99%

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A Simplified Gas Chromatographic Fatty‐Acid Analysis by the Direct Saponification/Methylation Procedure and Its Application on Wild Tuna Larvae

Matsumoto

Ando

Hiraoka

et al. 2018

Lipids

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A method for the direct preparation of fatty‐acid methyl esters (FAME) was simplified for fatty‐acid analysis of a single fish larva using gas chromatography (GC). The method included the isolation of a larval trunk and drying in a glass vial, followed by saponification of all the contents without prior lipid extraction. Thereafter, the fatty acids released were methylated by trimethylsilyldiazomethane. This method has advantages over another method, direct acid‐catalyzed transesterification, because both the saponification and methylation at room temperature can reduce loss of unsaturated fatty acids and formation of artifacts unavoidable in acidic reaction at high temperature. GC of the products showed that the simplified method can yield methyl esters without artifacts interfering analysis. More than 50 fatty acids were determined, which are twice as many as those previously analyzed using high‐performance liquid chromatography. Observation of consistent small impurities in GC of blank tests allowed the accurate determination of fatty acids by correcting the peak areas. Dry matter weights (<3 mg) and the total fatty‐acid contents displayed a linear relationship. Fatty‐acid analysis of wild larvae of bluefin tuna, yellowfin tuna, and skipjack tuna collected from the waters around Japan (n = 100) revealed that the eicosapentaenoic acid (EPA) level in bluefin tuna collected from the Japan Sea was significantly higher than that in the three species collected from Nansei Islands. The simplified direct saponification/methylation method will be a powerful tool for investigating growth and survival of individual larval tuna and other fish species.

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“…a). These values were comparable to previous findings observed in the internal validation of the original method (Juárez et al, ).…”

Section: Resultssupporting

confidence: 92%

Section: Validation Of Gc Analysismentioning

confidence: 99%

A Simplified Gas Chromatographic Fatty‐Acid Analysis by the Direct Saponification/Methylation Procedure and Its Application on Wild Tuna Larvae

Matsumoto

Ando

Hiraoka

et al. 2018

Lipids

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“…In this study, results obtained by both methods (B&D and SOXTEC) are comparable with data from the aforementioned studies. Among the most widely used methods for fat extraction from fish, there is B&D (18,19) and SOXTEC is another method used to extract fat not only from fish but from different matrixes; both methods are considered accurate and precise (13). When comparing the fat extraction from fish methods, the CRM recovery percentage was taken as indicator.…”

Section: Discussionmentioning

confidence: 99%

COMPARISON AND VALIDATION OF a FAST METHOD FOR THE EXTRACTION AND QUANTIFICATION OF Α-Linolenic, EICOSAPENTAENOIC, AND DOCOSAHEXAENOIC FATTY ACIDS

Castrillón

Laverde

Rodríguez

et al. 2016

Vitae

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Background: Omega 3 family fatty acids are currently widely studied for the diverse effects they have on cardiovascular disease risk factors. Seafood is the main natural source of these compounds. Objetives: In this paper the accuracy is verified on the fat extraction in fish, between two methods, using one of the most employees as it is the Bligh & Dyer since 1959, compared with SOXTEC, one more updated method with more modern equipment and validated quantification method for some omega 3 fatty acids by gas chromatography. Methods: Fat extraction was performed by SOXTEC and Bligh & Dyer with the use of a Certified Reference Material (SRM 1946). After having assessed the accuracy of both systems, a quantification method of α-Linolenic, Eicosapentaenoic, and Docosahexaenoic fatty acids was validated through gas chromatography. Results: It was found that the method proposed by Bligh & Dyer in 1959 is the most accurate because it showed better recovery percentages. Also, it was found that the quantification validation method of the most important omega 3 fatty acids complies with every parameter assessed throughout the validation. Conclusions: In conclusion SOXTEC being a more modern method which uses the latest technology, the Bligh & Dyer is the most effective method for removing fat in fish and gas chromatography allows quantification of fatty acids with precision and accuracy as meets the evaluated parameters.

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“…The composition and extraction of PUFAs from algae (Plaza et al 2009 ) , fi sh (Sahena et al 2009 ) , fi sh by-products ( Wu and Bechtel 2008 ) , and other marine sources (Juárez et al 2010 ) have been studied previously. However, PUFAs are prone to lipid oxidation and this represents one drawback for their inclusion in functional foods.…”

Section: Lipidsmentioning

confidence: 99%

Extraction and Characterization of Bioactive Compounds with Health Benefits from Marine Resources: Macro and Micro Algae, Cyanobacteria, and Invertebrates

Ibáñez

Herrero

Mendiola

et al. 2011

Marine Bioactive Compounds

201

178

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Validation of a gas–liquid chromatographic method for analysing samples rich in long chain n−3 polyunsaturated fatty acids: Application to seafood

Cited by 13 publications

References 43 publications

A Simplified Gas Chromatographic Fatty‐Acid Analysis by the Direct Saponification/Methylation Procedure and Its Application on Wild Tuna Larvae

A Simplified Gas Chromatographic Fatty‐Acid Analysis by the Direct Saponification/Methylation Procedure and Its Application on Wild Tuna Larvae

COMPARISON AND VALIDATION OF a FAST METHOD FOR THE EXTRACTION AND QUANTIFICATION OF Α-Linolenic, EICOSAPENTAENOIC, AND DOCOSAHEXAENOIC FATTY ACIDS

Extraction and Characterization of Bioactive Compounds with Health Benefits from Marine Resources: Macro and Micro Algae, Cyanobacteria, and Invertebrates

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