PRODUCTION AND CHARACTERIZATION OF THERMOPHILIC CARBOXYMETHYL CELLULASE SYNTHESIZED BY Bacillus sp. GROWING ON SUGARCANE BAGASSE IN SUBMERGED FERMENTATION

Bio-ethanol is considered as an important renewable fuel to partly replace fossil-derived fuels. In this study, bioethanol production, which includes cellulase production, saccharification of the cellulose content of sesame seed residue, and ethanol production, was investigated. Out of the hundreds of cellulase-producing bacterial strains isolated from sesame seed residue during this study, the B isolate was found to have the highest cellulase enzyme production. This isolate was identified as Bacillus cereus by 16S rRNA sequencing. The effects of different growth parameters, including inoculum concentration, incubation time, temperature, pH, and carbon and nitrogen sources, were investigated to optimize the growth conditions of the bacterifum. The maximum cellulase activity was achieved with an inoculum concentration of 3% after 48 h in a basal medium at a pH of 7 and an incubation temperature of 35 °C. The best nitrogen and carbon sources were yeast extract and sesame seed residue, respectively. The results showed the liberation of 2.3 g/L of reducing sugar by the dinitrosalicylic acid method. This total reducing sugar produced 15 g/L of ethanol after 48 h when Saccharomyces cerevisiae was used as a fermentation agent. Hence, bioethanol was successfully produced from the cellulose of sesame seed residue using the cellulase enzyme from B. cereus.

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“…Moreover, the highest cellulase activity of the Bacillus sp. was reached at a pH of 7.0, which was reported by Padilha et al (2015).…”

Section: Effect Of the Phsupporting

confidence: 63%

Bioethanol Production with Cellulase Enzyme from Bacillus cereus Isolated from Sesame Seed Residue from the Jazan Region

et al. 2018

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“…The maximum CMCase activity of 0.29 IU/mL was reported in a study on cellulase production by Bacillus sp. at pH 7 [58]. From previous studies, only few xylanases have been reported to be active and stable at alkaline pH and high temperatures [59,60].…”

Section: Discussionmentioning

confidence: 99%

Optimization of Cellulase and Xylanase Production by Micrococcus Species under Submerged Fermentation

et al. 2016

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This paper reports on the optimization of culture conditions for cellulase and xylanase production by bacterial isolate from lignocellulosic biomass. The bacterial isolate was screened for cellulase and xylanase production on carboxyl methyl cellulose (CMC) and birch wood xylan as substrates, respectively. One bacterial isolate showing the highest halo zone diameter (isolate PLY1) was selected for detailed studies. The analysis of the 16S ribosomal ribonucleic acid (rRNA) gene nucleotide sequence of PLY1 revealed it to have 98% similarity to Micrococcus luteus strain Fse9 and the sequence was deposited in the GenBank as Micrococcus luteus strain SAMRC-UFH3 with accession number KU171371. Cellulase production was achieved in the presence of CMC (1% w/v) under an incubation temperature of 25 • C (198 U/mL), pH 5 (173 U/mL), agitation speed 50 rpm (173 U/mL) and incubation period of 96 h (102 U/mL). Xylanase was produced maximally when birch wood xylan (1% w/v) was used as the substrate at 25 • C (1007 U/mL), pH 10 (2487 U/mL), 200 rpm (1814 U/mL), and under an incubation period of 84 h (1296 U/mL). Our findings showed that Micrococcus sp. SAMRC-UFH3 appears to be a potentially important candidate for lignocellulosic waste degradation and other relevant industrial applications.

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“…MSS contained (g/l) NaNO 3 0.5, K 2 HPO 4 1.0, MgSO 4 .7H 2 O 0.5, FeSO 4 .7H 2 O 0.01, and yeast extract 1.0 containing 0.5% CMC at pH 7. Cultures were incubated for 24 h at 60°C and 180 rpm [21]. Culture was centrifuged at ×12000 g for 20 min at 4°C.…”

Section: Cellulase Productionmentioning

confidence: 99%

Morphological, enzymatic screening, and phylogenetic analysis of thermophilic bacilli isolated from five hot springs of Myagdi, Nepal

2018

J App Biol Biotech

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The present study was conducted to identify and characterize the thermophilic bacteria isolated from five hot springs, namely, Sinkosh, Singha, Bhurung, Ratopani, and Paudwar located in Myagdi district, Nepal, using phenotypic and genotypic methods. The hot spring has temperature 42-62°C and pH 6.5-6.8. Isolation of thermophiles was done using simple enriched nutrient broth media at 60°C. Selected strains were screened for thermostable enzymes; cellulase, hemicellulase, amylase, protease, gelatinase, and lipase using substrates carboxymethylcellulose, xylan, soluble starch, casein, gelatin, and Tween (20, 40, 60, or 80), respectively. The bacteria were grouped into 16 groups based on morphological and biochemical characteristics. 16S rRNA sequence analysis of 16 isolates and phylogenetic analysis showed a cluster of five distinct taxonomic groups. The groups were identified as genus Anoxybacillus, Aeribacillus, Brevibacillus, Bacillus, and Geobacillus, based on ≥95% similarity with reference strains. This is the first study that reports Anoxybacillus sp., Brevibacillus sp., and Aeribacillus sp. from the hot springs of Nepal.

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PRODUCTION AND CHARACTERIZATION OF THERMOPHILIC CARBOXYMETHYL CELLULASE SYNTHESIZED BY Bacillus sp. GROWING ON SUGARCANE BAGASSE IN SUBMERGED FERMENTATION

Cited by 35 publications

References 30 publications

Bioethanol Production with Cellulase Enzyme from Bacillus cereus Isolated from Sesame Seed Residue from the Jazan Region

Bioethanol Production with Cellulase Enzyme from Bacillus cereus Isolated from Sesame Seed Residue from the Jazan Region

Optimization of Cellulase and Xylanase Production by Micrococcus Species under Submerged Fermentation

Morphological, enzymatic screening, and phylogenetic analysis of thermophilic bacilli isolated from five hot springs of Myagdi, Nepal

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