2018
DOI: 10.7324/jabb.2018.60301
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Abstract: The present study was conducted to identify and characterize the thermophilic bacteria isolated from five hot springs, namely, Sinkosh, Singha, Bhurung, Ratopani, and Paudwar located in Myagdi district, Nepal, using phenotypic and genotypic methods. The hot spring has temperature 42-62°C and pH 6.5-6.8. Isolation of thermophiles was done using simple enriched nutrient broth media at 60°C. Selected strains were screened for thermostable enzymes; cellulase, hemicellulase, amylase, protease, gelatinase, and lipas… Show more

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Cited by 10 publications
(6 citation statements)
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“…These findings are consistent with studies conducted in several hot springs and suggest that the presence of Bacillus in the sampled sites may be due to the ability of the genus to withstand adverse environmental conditions (Connor et al, 2010;Mohammad et al, 2017;Sahay et al, 2017;Yadav et al, 2018). Furthermore, the physical and chemical characteristics of the aquifer, the possibilities of water replenishment and sampling site seem to determine the microbial community present (Chiriac et al, 2017;Chiriac et al, 2018).…”
Section: Resultssupporting
confidence: 86%
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“…These findings are consistent with studies conducted in several hot springs and suggest that the presence of Bacillus in the sampled sites may be due to the ability of the genus to withstand adverse environmental conditions (Connor et al, 2010;Mohammad et al, 2017;Sahay et al, 2017;Yadav et al, 2018). Furthermore, the physical and chemical characteristics of the aquifer, the possibilities of water replenishment and sampling site seem to determine the microbial community present (Chiriac et al, 2017;Chiriac et al, 2018).…”
Section: Resultssupporting
confidence: 86%
“…Thermophilic microorganisms have attracted attention as sources of thermostable enzymes, such as amylases, cellulases, chitinases, pectinases, xylanases, proteases, lipases and DNA polymerases, which have characteristics appropriate to the extreme processes used in industry (Mohammad et al, 2017). The global demand for enzymes is growing rapidly and different thermo-active enzymes produced by microorganisms find its applications in various industries such as food, detergent, pharmaceutical and cosmetic, biofuels, leather processing, pulp and paper, textile industry and for studies in biology (Torimiro and Okonji, 2013;Gopinath et al, 2017;Oumer and Abate, 2017;Sahay et al, 2017;Yadav et al, 2018). In addition to enzymes, a variety of other compounds with Brazilian Journal of Development, Curitiba, v.7, n.1, p. 8219-8236 Jan. 2021 pharmacological properties such as antibiotics, antifungals and anti-inflammatories have been identified in bacteria living in complex ecological communities, such as those found in terrestrial and aquatic environments (Jiménez-Delgadillo et.…”
Section: Introductionmentioning
confidence: 99%
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“…Finally, all the isolates were catalase and oxidase positive (except E. faecium and E. profundum). Yadav 57 carried out the isolation and identification of 150 thermophilic bacteria from hot springs in Nepal. The 16S rRNA fragments of the isolates were amplified with a size of about 1.5 kbs (1500 bp) and the microorganisms were belonging to the genus Anoxybacillus, Aeribacillus, and Bacillus at a ratio of ≥95.…”
Section: Morphology Physiology and Biochemical Characteristics Of Thmentioning
confidence: 99%
“…A. geothermalis, A. mongoliensis, B. licheniformis and B. paralicheniformis were able to produce xylanase; however, A. geothermalis, A. mongoliensis and B. paralicheniformis were firstly determined as thermo-tolerant xylanase producers When the multienzyme production capabilities of the isolates were examined, it was observed that all the isolates, except B. halodurans, E. profundum and P. dendritiformis, were able to produce more than one enzyme. In a study, in order to determine the strains which have the potential to produce industrially important enzymes, Yadav 57 observed that 135 of 150 thermophilic isolates have the potential to produce at least one extracellular hydrolytic enzyme.…”
Section: Enzyme Production Capacities Of Thermophilesmentioning
confidence: 99%