Production and Characterization of the Monoclonal Antibodies to Rickettsia tsutsugamushi

Transfiguration of Rickettsial Diseases: Tsutsugamushi Disease and Spotted Fever Group Rickettsiosis in Japan.

“…The serological reactivity of the anti-Gilliam, anti-Karp and anti-Kato monoclonal antibodies showed varied reactive characteristics, i.e., serotype-specific , species-specific and intermediate reactivities (2). The reactivity of these monoclonal antibodies against the newly isolated strains of Miyazaki, Niigata and Ehime Prefectures revealed that the strains of Ehime were identified as the Karp or Kato type (3), but the other strains were serologically different from the classic ones (4).…”

Newly isolated strains of Rickettsia tsutsugamushi in Japan identified by using monoclonal antibodies to Karp, Gilliam, and Kato strains

“…We isolated four strains of Rickettsia tsutsugamushi from patients in this area to investigate the nature of these strains, not only to detect the prevalent strains in this area but also to speculate on the real cause of the sudden emergence of the disease in this prefecture. Recent studies suggested the usefulness of murine monoclonal antibodies (MAbs) to standard strains (Karp, Gilliam, and Kato) of R. tsutsugamushi for classifying the isolates (1,2,7). In this study, the antigenicities of the new isolates were analyzed by using either strain-specific or cross-reactive MAbs to standard strains.…”

“…Moreover, reactivity against cross-reactive Kp/lC1O was critical between the two strains. In recent years, several investigators reported new isolates which were antigenically distinguished from standard strains (2,5,11). Kobayashi et al (4) reported newly isolated strains which were identified as the Gilliam-Kato type by using the IFA technique with MAbs to Karp, Gilliam, and Kato strains.…”