Production and Characterization of Serratiopeptidase Enzyme from Serratia Marcescens

Abstract

Production characterization of serratiopeptidase (STP) enzyme by Serratia marcescens was the aim of this study. Serratia marcescens was allowed to grow in Tryptone Yeast Extract Glucose broth culture for the purpose of inducing STP (serrapeptase or serratiopeptidase) enzyme. The optimal conditions for STP production by Serratia marcescens were; 0.5 % substrate(Gelatin) concentration; 24 h incubation period; 32ºC incubation temperature and 6.0 pH ; the best buffer for production of STP enzyme was phosphate buffer. The best broth ingredient was tryptone; An optimum carbon sources was glucose; an optimum nitrogen source for STP enzyme production was tryptone; Valine was the best amino acids for the production of STP enzyme; the utilization of organic acids, acetic, citric, lactic acid decreased STP enzyme production at different concentrations above 3.0%. The STP enzyme was partially purified by ammonium sulfate precipitation and dialysis. The enzyme was found to have 52 KDa molecular weight by SDS -PAGE analysis. The STP enzyme activity increased as the increase in enzyme concentration. The data obtained emphasizes the possibility of production and purification of the microbial STP enzyme for application under industrial scale.

Production and Partial Characterization of Fibrinolytic Enzyme From a Soil Isolate Aspergillus Carbonarius S-CSR-0007

“…Optimum enzyme activity of enzyme production in this study was seen at pH 5.0, Schizophyllum commune BL23 and in Serratia marcescens [9] showed maximum enzyme production at pH 6.0. Cordyceps militaris showed maximum enzyme production at pH 7.0 [17].…”

“…Precipitated protein was removed by centrifugation at 1,000 rpm for 20 min at 4 °C. Then the supernatant was discarded [9]. The precipitated protein was dissolved in 50 mmol Tris-HCl (10 ml), pH 7.0, assayed for determining enzyme activity and protein content [7].…”

Microbial Enzymes: Therapeutic Applications

“…Serratiopeptidase hydrolyses bradykinin, histamine and serotonin responsible for the oedematic status. Serratiopeptidase reduces swelling, improves microcirculation and expectoration of sputum, etc [34].…”

Clove Oil Emulsified Buccal Patch of Serratiopeptidase for Controlled Release in Toothache

“…The serratiopeptidase acts by hydrolyzing the histamine, bradykinin and serotonin and uses for pain and inflammatory conditions such as arthritis and minor muscle trauma. It can absorb through intestines and transported directly in to blood stream [3,4]. Clove oil is an essential oil and is obtained from the dried flowers, buds, leaves, stem of the tree Syzygium aromaticum or Eugenia cariophyllata and Eugenia aromaticum.…”