Production and characterization of “second generation” somatic hybrids derived from protoplast fusion between interspecific somatohaploid and dihaploidSolanum tuberosum L.

Rokka, Veli-Matti; Valkonen, Jari P. T.; Tauriainen, Airi; Pietilä, Leena; Lebecka, Renata; Zimnoch‐Guzowska, E.; Pehu, Eija

doi:10.1007/bf02853939

Cited by 7 publications

(5 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Subsequently, the interspecific somatic hybrids were subjected to haploid-induction via anther culture in order to reduce their ploidy levels (Rokka et al 1995, Gavrilenko et al 2002. The new 'somatohaploid' plants were used for production of 'second-generation' somatic hybrids with dihaploid potato lines (Rokka et al 2000, Gavrilenko et al 2002. Hence, the cultivated potato gene pool and thereby fertility and chances to make sexual crosses with potato breeding lines were increased.…”

Section: Potato Biotechnologymentioning

confidence: 99%

Plant biotechnology for deeper understanding, wider use and further development of agricultural and horticultural crops

Elomaa¹,

Joensuu²,

Korpelainen³

et al. 2008

AFSci

View full text Add to dashboard Cite

Plants bind solar energy to organic matter via photosynthesis and assimilation of carbon dioxide from the atmosphere and comprise the major source of nutrition and bioenergy. Plant biotechnology contributes to solution of important constraints in food and feed production and creates new technologies and applications for the sustainable use of plant resources. Genome-wide approaches such as massive parallel sequencing and microarrays to study gene expression, molecular markers for selection of important traits in breeding, characterization of genetic diversity with the aforementioned approaches, and somatic hybridization and genetic transformation are important tools in plant biotechnology. In this paper, studies carried out on enhanced resistance to viruses and tolerance of cold stress in potato, genetic modification of flower pigmentation and morphology in gerbera, production of edible vaccines in transgenic barley seeds, and expression of heterologous proteins for pharmaceutical purposes from vector viruses were chosen to exemplify the general utility of biotechnological approaches and also how plant biotechnology research has developed on cultivated plants at University of Helsinki. The studies reveal cellular and genetic mechanisms and provide scientific information that can be used for widening the uses of crop plants. They can also be used to detect any putative risks associated with the use of the biotechnological application in agriculture and horticulture and to develop practises which reduce any inadvertent negative consequences that plant production may have to the environment.

show abstract

Section: Potato Biotechnologymentioning

confidence: 99%

Plant biotechnology for deeper understanding, wider use and further development of agricultural and horticultural crops

Elomaa¹,

Joensuu²,

Korpelainen³

et al. 2008

AFSci

View full text Add to dashboard Cite

show abstract

“…All these resistance traits carried out by the hybrid lines can be of great interest to increasing the genetic diversity available for potato breeding, but we must emphasize that these hybrids are not yet commercial potato cultivars and that backcrosses or additional back‐fusions are needed to limit the risk of undesirable wild potato characteristics (Rokka et al. , ; Simko et al. ).…”

Section: Discussionmentioning

confidence: 99%

Somatic Hybrids Between Potato and S. berthaultii Show Partial Resistance to Soil‐Borne Fungi and Potato Virus Y

Nouri-Ellouz

Triki²,

Jbir-Koubaa³

et al. 2016

Journal of Phytopathology

View full text Add to dashboard Cite

Three tetraploid somatic hybrid lines produced by protoplast fusion between a dihaploid potato, Solanum tuberosum, cultivar BF15 and the wild potato species Solanum berthaultii were evaluated here for their response to different soil‐borne pathogens, that is Fusarium solani, Pythium aphanidermatum and Rhizoctonia solani as well as to infection by potato virus Y (PVY). Both hybrid and BF15 plants grown in vitro were inoculated with the tested pathogen strains, that is R. solani, P. aphanidermatum, or F. solani. The growth level and disease severity index of these plants were compared to the susceptible commercial cultivar Spunta. A better growth of inoculated hybrid plants and restricted disease symptoms were observed in comparison with the commercial plants. Under glasshouse conditions and after inoculation with R. solani and P. aphanidermatum, improved resistance of the hybrid plants to these pathogens was confirmed. Indeed, these plants showed no significant damage following inoculation and a better development in R. solani‐infected plants. The susceptibility of the hybrid tubers to R. solani, P. aphanidermatum, and to F. solani infection was also determined. A significant reduction of tissue colonisation was observed in all the hybrid lines compared to the cultivated cultivars. The STBc and STBd hybrids also showed improved resistance to the PVY ordinary strain (PVYo) under glasshouse conditions.

show abstract

“…Despite numerous previous reports of the successful production of interspecific primary somatic hybrids, introgression of the alien genetic material into potato breeding lines has been considerably limited, mainly owing to reduced fertility (Waara and Glimelius 1995). To overcome the high sterility level in the interspecific hybrids, an alternative to the sexual-backcrossing breeding scheme to incorporate desirable traits from wild species S. brevidens into cultivated potato genomic background was proposed, using repeated somatic hybridizations and haploid formations (Rokka et al 1995(Rokka et al , 2000. In addition, the purpose was to decrease the dosage of alien S. brevidens genome of the primary somatic hybrids.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, five pentaploid (2n = 5x) second-generation somatic hybrids (2701-0105, 2701-0107, 2701-0109, 2701-0302, and 2701-0502), derived from a fusion between triploid somatohaploid 0507.1.2.1. and a dihaploid line of S. tuberosum cv. Van Gogh ('Van Gogh 11') (Rokka et al 2000), were included in the study. Details of the plant material are also presented in Table 1.…”

Section: Plant Materialsmentioning

confidence: 99%

“…Also, in our previous studies, (somato)haploid lines have been produced from male sterile S. tuberosum + S. brevidens somatic hybrids through androgenesis for potato improvement (Rokka et al 1995). A new strategy was subsequently proposed and tested involving the refusion of the somatohaploids with dihaploid lines of cultivated potato, resulting in second-generation somatic hybrids (Rokka et al 2000).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of mitotic chromosomes of tuberous and non-tuberousSolanumspecies (Solanum tuberosumandSolanum brevidens) by GISH in their interspecific hybrids

Гавриленко

Larkka²,

Pehu³

et al. 2002

Genome

View full text Add to dashboard Cite

GISH (genomic in situ hybridization) was applied for the analysis of mitotic chromosome constitutions of somatic hybrids and their derivatives between dihaploid clones of cultivated potato (Solanum tuberosum L.) (2n = 2x = 24, AA genome) and the diploid, non-tuberous, wild species Solanum brevidens Phil. (2n = 2x = 24, EE genome). Of the primary somatic hybrids, both tetraploid (2n = 4x) and hexaploid (2n = 6x) plants were found with the genomic constitutions of AAEE and AAEEEE, respectively. Androgenic haploids (somatohaploids) derived from the tetraploid somatic hybrids had the genomic constitutions of AE (2n = 2x = 24) and haploids originating from the hexaploid hybrids were triploid AEE (2n = 3x = 33 and 2n = 3x = 36). As a result of subsequent somatic hybridization from a fusion between dihaploid S. tuberosum (2n = 2x = 24, genome AA) and a triploid somatohaploid (2n = 3x = 33, genome AEE), second-generation somatic hybrids were obtained. These somatic hybrids were pentaploids (2n = 5x, genome AAAEE), but had variable chromosome numbers. GISH analysis revealed that both primary and second-generation somatic hybrids had lost more chromosomes of S. brevidens than of S. tuberosum.

show abstract

Production and characterization of “second generation” somatic hybrids derived from protoplast fusion between interspecific somatohaploid and dihaploidSolanum tuberosum L.

Cited by 7 publications

References 41 publications

Plant biotechnology for deeper understanding, wider use and further development of agricultural and horticultural crops

Plant biotechnology for deeper understanding, wider use and further development of agricultural and horticultural crops

Somatic Hybrids Between Potato and S. berthaultii Show Partial Resistance to Soil‐Borne Fungi and Potato Virus Y

Identification of mitotic chromosomes of tuberous and non-tuberousSolanumspecies (Solanum tuberosumandSolanum brevidens) by GISH in their interspecific hybrids

Contact Info

Product

Resources

About