Production and characterization of polyhydroxybutyrate from<i>Vibrio harveyi</i>MCCB 284 utilizing glycerol as carbon source

Mohandas, Sowmya P.; Balan, Linu; Lekshmi, N. C. J. Packia; Cubelio, Sherine Sonia; Philip, Rosamma; Singh, I. S. Bright

doi:10.1111/jam.13359

Cited by 44 publications

(42 citation statements)

References 35 publications

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“…Poly-âhydroxybutyrate obtained using molasses in this study was 6.36 g/L. Sowmya et al, [17] reported maximum PHB yield of 1.2 g/L at optimum conditions of pH 8.0, sodium chloride concentration 20 g l -1 , inoculum size 0.5% (v/v), glycerol 20 g l -1 and 72 h of incubation at 30°C. In comparison with these previous reports, the PHB production from RR20 strain (18)(19)) is said to be higher and raw materials of medium are also expected to reduce the estimated cost of the process.…”

Section: Results and Discussion Face Centred Central Composite Designsupporting

confidence: 53%

Statistical optimization of PolyHydroxy Butyrate (PHB) production by novel Acinetobacter nosocomialis RR20 strain using Response Surface Methodology

Reddy¹,

Prabhakar²,

Venkateswarulu³

et al. 2020

CTBP

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Eco-friendly biopolymers, polyhydroxybutyrate, have been produced by many kinds of bacteria that possess most important applications in food packaging industries and also in medical field. In the present work, PHB production was economized through the statistical optimization of physical variables. The physical variables chosen for the enhanced production of PHB were incubation period, temperature, pH and inoculums size. The relative rate of production has been studied by understanding the complex interactions of variables using face-centred central composite design. The submerged fermentation with Acinetobacter nosocomialis RR20 produced PHB yield of 5.88 g/L at optimized conditions with a notable value change of sixfold increase in comparison with minimal salt medium and these findings have showed that the designed medium was significant in terms of higher of PHB production.

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Section: Results and Discussion Face Centred Central Composite Designsupporting

confidence: 53%

Statistical optimization of PolyHydroxy Butyrate (PHB) production by novel Acinetobacter nosocomialis RR20 strain using Response Surface Methodology

Reddy¹,

Prabhakar²,

Venkateswarulu³

et al. 2020

CTBP

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“…Polyhydroxyalkanoates (PHAs) are a class of natural polyesters that are produced as carbon/energy or reducing‐power storage material by numerous micro‐organisms with an essential growth‐limiting component such as nitrogen, phosphate, sulfur, oxygen, or magnesium . Two commercial PHA polymers, polyhydroxybutyrate (PHB, Figure a) and poly(hydroxybutyrate‐ co‐ hydroxyvalerate) (PHBV, Figure b), have been studied most in recent years.…”

Section: Introductionmentioning

confidence: 99%

Surface Modification of Polyhydroxyalkanoates toward Enhancing Cell Compatibility and Antibacterial Activity

Liu

Zhang

et al. 2017

Macro Materials & Eng

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Biomaterials for in vivo application should induce positive interaction with various histocytes and inhibit bacteria inflection as well. Cells and/or bacteria response to the extracellular environment is therefore the basic principle to design the biomaterials surface in order to induce the specific biomaterial–biological interaction. Polyhydroxyalkanoate (PHAs) are of growing interests because of their natural origin, biodegradability, biocompatibility, and thermoplasticity; however, quite inert and intrinsic hydrophobic characteristics have hindered their extensive usage in medical applications. Surface modification of PHAs tailors the chemistry, wettability, and topography without altering the bulk properties, and introduces specific proteins/peptides and/or antibacterial agents to mediate cell–matrix interactions. This review describes the recent developments on the surface modification of PHAs to construct cell compatible and antibacterial surfaces.

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“…Meanwhile, in the medical field, antimicrobial materials consisting of PHB nanocomposites and silver nanoparticles have been biosynthesized 10 and used as drug carriers for wound management and tissue engineering 11 . The structural characterization of intracellular polymers can be performed using UV spectrophotometry, nuclear magnetic resonance (NMR) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, gas chromatography-mass spectrometry (GC-MS), and differential scanning calorimetry 7,[12][13][14][15] . Economically feasible and sustainable microbial strains, substrates, industrial processes, and extraction methods for PHB are still being developed 4 .…”

mentioning

confidence: 99%

“…Indeed, the different types of bacterial communities in the Red Sea are reported to be a source of potentially useful bioproducts for biotechnological and pharmaceutical applications, and the gene responsible for PHB synthase exhibits relatively high abundance in the microorganisms living in the Red Sea 19 . The marine bacterial species Vibrio harveyi 14 , Paracoccus species, Micrococcus species 21 , Erythrobacter aquimaris 22 , Halomonas elongata 23 , Bacillus megaterium 24 , Cupriavidus necator 25 , and Haloferax mediterrani 26 have been found to be potent producers of bioplastics. Consequently, Red Sea habitats are regarded as a potential source for novel bacterial strains that exhibit efficient PHB production.…”

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confidence: 99%

Bioplastic (poly-3-hydroxybutyrate) production by the marine bacterium Pseudodonghicola xiamenensis through date syrup valorization and structural assessment of the biopolymer

Mostafa

Alrumman

Alamri

et al. 2020

Sci Rep

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Biobased degradable plastics have received significant attention owing to their potential application as a green alternative to synthetic plastics. A dye-based procedure was used to screen poly-3hydroxybutyrate (PHB)-producing marine bacteria isolated from the Red Sea, Saudi Arabia. Among the 56 bacterial isolates, Pseudodonghicola xiamenensis, identified using 16S rRNA gene analyses, accumulated the highest amount of PHB. The highest PHB production by P. xiamenensis was achieved after 96 h of incubation at pH 7.5 and 35 °C in the presence of 4% NaCl, and peptone was the preferred nitrogen source. The use of date syrup at 4% (w/v) resulted in a PHB concentration of 15.54 g/L and a PHB yield of 38.85% of the date syrup, with a productivity rate of 0.162 g/L/h, which could substantially improve the production cost. Structural assessment of the bioplastic by Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy revealed the presence of methyl, hydroxyl, methine, methylene, and ester carbonyl groups in the extracted polymer. The derivative products of butanoic acid estimated by gas chromatography-mass spectrometry [butanoic acid, 2-amino-4-(methylseleno), hexanoic acid, 4-methyl-, methyl ester, and hexanedioic acid, monomethyl ester] confirmed the structure of PHB. The present results are the first report on the production of a bioplastic by P. xiamenensis, suggesting that Red Sea habitats are a potential biological reservoir for novel bioplastic-producing bacteria. Petroleum-based plastic has been rapidly produced in recent decades, and its biodegradation resistance has led to a serious environmental issue for the management of solid wastes 1. The global demand for bioplastic as a substitute for synthetic plastics has increased due to its nontoxicity, renewability, biocompatibility, and biodegradability 2. Degradable biobased plastics can be produced from different renewable raw materials (polysaccharides and proteins), plants (starch-based plastics and cellulose-based plastics), and microbial bioplastics (polylactic acid and polyhydroxyalkanoates (PHAs)) 3. PHAs are the most promising type of bioplastic; they are nontoxic, biodegradable and biocompatible and have properties similar to those of conventional plastics 4. PHAs are biopolymers with diverse structures; as a defense mechanism for surviving stress conditions with nutrient imbalance, PHAs accumulate inside bacterial cells as stored energy 5. The considerable variations in functional groups from methyl to tridecyl, unsaturated bonds, and chain length make PHAs appropriate biopolymers for many different applications 6. The most common form of PHAs is poly-3-hydroxybutyrate (PHB), which accumulates in many microbes by binding β-hydroxybutyrate monomers with ester bonds 7. The universal manufacturing capacity of

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Production and characterization of polyhydroxybutyrate fromVibrio harveyiMCCB 284 utilizing glycerol as carbon source

Cited by 44 publications

References 35 publications

Statistical optimization of PolyHydroxy Butyrate (PHB) production by novel Acinetobacter nosocomialis RR20 strain using Response Surface Methodology

Statistical optimization of PolyHydroxy Butyrate (PHB) production by novel Acinetobacter nosocomialis RR20 strain using Response Surface Methodology

Surface Modification of Polyhydroxyalkanoates toward Enhancing Cell Compatibility and Antibacterial Activity

Bioplastic (poly-3-hydroxybutyrate) production by the marine bacterium Pseudodonghicola xiamenensis through date syrup valorization and structural assessment of the biopolymer

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