This is the first report of an isolate of V. harveyi which utilizes glycerol as the sole carbon source for PHB production with high biomass yield. This isolate could be of use as candidate species for commercial PHB production using glycerol as the feed stock or as source of genes for recombinant PHB production or for synthetic biology.

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For the sustainable farming of disease-free and healthy shrimps, antimicrobial usage is frequent nowadays in shrimp-cultured system. This could lead to the emergence of severe antimicrobial resistance (AMR) in the whole ecosystem. Considering the serious impact of global AMR, the present study was focused to investigate the prevalence of antimicrobial resistant vibrios among infected shrimps (Litopenaeus vannamei) from two brackish-water cultured farms. Diverse species of vibrios viz. V. alginolyticus, V. parahaemolyticus, V. cholerae, V. mimicus and V. uvialis and other isolates such as Aeromonas hydrophila, A. salmonicida and Shewanella algae were also recovered from the shrimps on TCBS medium.Shannon wiener diversity index, H' (loge) was found to be 1.506 for the isolates from farm 1 and 1.69 for those from farm 2. V. alginolyticus was found to be the most resistant isolate by showing MAR index of 0.60 followed by V. mimicus (0.54) and V. parahaemolyticus(0.42). Among the 35 antibiotics of 15 different classes tested, tetracyclines, beta lactams and cephalosporins were found as the most resistant antibiotic classes. An increased altered resistance phenotype and a drastic change in MAR index were noticed after plasmid curing. Since the studied shrimp samples are of signi cance in food sector and plasmid-borne AMR is evident among the isolates, public health is also alarming. This baseline information will help the authorities to curb the antimicrobial use and pave the way for establishing new alternative strategies by undertaking multidimensional "One-Health" approach.