Production and characterization of polycaprolactone- hyaluronic acid/chitosan- zein electrospun bilayer nanofibrous membrane for tissue regeneration

Figueira, Daniela Sofia Rodrigues; Miguel, Sónia P.; Sá, Kevin Domingos de; Correia, Ilídio J.

doi:10.1016/j.ijbiomac.2016.09.080

Cited by 139 publications

(75 citation statements)

References 85 publications

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“…However, similarly as in many other natural polymers, the application of pure zein nanofibers is limited because of poor mechanical properties of these fibers. Therefore, for skin tissue engineering and wound dressing applications, zein has been mixed with various synthetic and nature-derived polymers, such as polyurethane [161], PLA [162], PCL, hyaluronic acid, chitosan [163], and polydopamine [164], and impregnated with TiO 2 nanoparticles [164] or Ag nanoparticles [161] in order to enhance the antimicrobial activity of the scaffolds.…”

Section: Nature-derived Nanofibers Degradable In the Human Tissuesmentioning

confidence: 99%

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Nature-Derived Polymers

Bačáková¹,

Pajorová²,

Zikmundová³

et al. 2020

Current and Future Aspects of Nanomedicine

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Nanofibrous scaffolds belong to the most suitable materials for tissue engineering, because they mimic the fibrous component of the natural extracellular matrix. This chapter is focused on the application of nanofibers in skin tissue engineering and wound healing, because the skin is the largest and vitally important organ in the human body. Nanofibrous meshes can serve as substrates for adhesion, growth and differentiation of skin and stem cells, and also as an antimicrobial and moistureretaining barrier. These meshes have been prepared from a wide range of synthetic and nature-derived polymers. This chapter is focused on the use of nature-derived polymers. These polymers have good or limited degradability in the human tissues, which depends on their origin and on the presence of appropriate enzymes in the human tissues. Non-degradable and less-degradable polymers are usually produced in bacteria, fungi, algae, plants or insects, and include, for example, cellulose, dextran, pullulan, alginate, pectin and silk fibroin. Well-degradable polymers are usually components of the extracellular matrix in the human body or at least in other verte brates, and include collagen, elastin, keratin and hyaluronic acid, although some polymers produced by non-vertebrate organisms, such as chitosan or poly(3-hydroxybutyrate-co-3-hydroxyvalerate), are also degradable in the human body.Current and Future Aspects of Nanomedicine 2 microbial infection, and at the same time, they can keep appropriate moisture and gas exchange at the wound site.Nanofibrous scaffolds for skin tissue engineering have been fabricated from a wide range of synthetic and nature-derived polymers, which can be either biostable or degradable within the human body. Biostable synthetic polymers used in nanofiber-based skin regenerative therapies include, for example, polyurethane [2], polydimethylsiloxane [3], polyethylene terephthalate [4], polyethersulfone [5], and also hydrogels such as poly(acrylic acid) (PAA, [6]), poly(methyl methacrylate) (PMMA, [7]), and poly[di(ethylene glycol) methyl ether methacrylate] (PDEGMA, [8]). Degradable synthetic polymers typically include poly(ε-caprolactone) (PCL, [9]) and its copolymers with polylactides (PLCL, [10]), polylactides (PLA, [11]) and their copolymers with polyglycolides (PLGA, [12]), and also so-called auxiliary polymers, such as poly(ethylene glycol) (PEG), poly(ethylene oxide) (PEO, [13]) or poly(vinyl alcohol) (PVA, [14]), which facilitated the electrospinning process and improved the mechanical properties and wettability of the chief polymer. However, the synthetic polymers, although they are well-chemically defined and tailorable, are often bioinert, hydrophobic and thus not promoting cell adhesion, and also not well-adhering to the wound site. Therefore, they need to be combined with other bioactive substances, particularly nature-derived polymers.This chapter is focused on nature-derived polymers used for fabrication of nanofibrous scaffolds for skin tissue engineering and wound healing. The advantages of...

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Section: Nature-derived Nanofibers Degradable In the Human Tissuesmentioning

confidence: 99%

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Nature-Derived Polymers

Bačáková¹,

Pajorová²,

Zikmundová³

et al. 2020

Current and Future Aspects of Nanomedicine

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“…Correia and co‐workers produced an innovative bilayered electrospun membrane composed of hyaluronic acid and polycaprolactone (PCL) in the top layer and chitosan and zein in the bottom layer . The hyaluronic acid and PCL served as mechanical supports and physical barriers for protection against the external environment, while the bottom layer loaded with salicylic acid along with chitosan and zein provided anti‐inflammatory and antimicrobial activity to that part of the membrane . The fabricated membrane had a porosity greater than 90%, which is ideal for cell seeding and proliferation .…”

Section: Hydrogel Delivery Strategiesmentioning

confidence: 99%

“…The hyaluronic acid and PCL served as mechanical supports and physical barriers for protection against the external environment, while the bottom layer loaded with salicylic acid along with chitosan and zein provided anti‐inflammatory and antimicrobial activity to that part of the membrane . The fabricated membrane had a porosity greater than 90%, which is ideal for cell seeding and proliferation . Human fibroblast cells remained viable and were able to adhere to the membrane and proliferate.…”

Section: Hydrogel Delivery Strategiesmentioning

confidence: 99%

“…In summary, the combination of synthetic and natural polymers in the electrospun membrane scaffold promoted desirable properties for wound dressings. Future work involves incorporating growth factors, vitamins, cytokines, and other biomolecules into the scaffold to enhance the wound healing process and improve the performance of the membrane …”

Section: Hydrogel Delivery Strategiesmentioning

confidence: 99%

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Crafting Polymeric and Peptidic Hydrogels for Improved Wound Healing

Stern

Cui

2019

Adv Healthcare Materials

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Wound healing is a multifaceted biological process involving the replacement of damaged tissues and cellular structures, restoring the skin barrier's function, and maintaining internal homeostasis. Over the past two decades, numerous approaches are undertaken to improve the quality and healing rate of complex acute and chronic wounds, including synthetic and natural polymeric scaffolds, skin grafts, and supramolecular hydrogels. In this context, this review assesses the advantages and drawbacks of various types of supramolecular hydrogels including both polymeric and peptide‐based hydrogels for wound healing applications. The molecular design features of natural and synthetic polymers are examined, as well as therapeutic‐based and drug‐free peptide hydrogels, and the strategies for each system are analyzed to integrate key elements such as biocompatibility, bioactivity, stimuli‐responsiveness, site specificity, biodegradability, and clearance.

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“…Polymers such as polycaprolactone (PCL) are defined as a synthetic, semicrystalline, hydrophobic, biocompatible and highly permeable polymer [22] that has as its property a slow degradation [23] and great utility in the medical area for sutures and controlled delivery of drugs [24].…”

mentioning

confidence: 99%

Synthesis and morphological characterization of Polycaprolactone (PCL) membranes with tara extract (Caesalpinia spinosa)

Silva

Sato

Borges

2019

BDS

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Objective: This study aimed the synthesis and morphological characterization of PCL electrospun fibers containing tara extract. Material and Methods: For this, tara extract synthesis was performed by two different extraction methods: rotary evaporator and extractor soxhlet. Then, two solutions were prepared by dissolving 3g of PCL in 2mL of Acetone. The first solution used 0.4 mL tara extract obtained by RE and the second solution used 0.4 mL tara extract obtained by SE. After the solutions electrospinning, under different parameters, obtaining It was obtained the experimental groups: ChTa 1 nanofibers with RE extract, under 12 Kv; ChTa 2 nanofibers with RE extract, under 15 Kv; ChTa 3 nanofibers with ES extract, under 12Kv and ChTa 4 nanofibers with ES extract, under 15kV. Scanning electron micrographs were performed for morphological analysis. Results: Fiber formation was observed for all parameters. About the fiber diameter: ChTa 1 presented a mean of 0.82 ± 0.36μm, ChTa 2 1.232 ±0471μm, ChTa 3 1.469 ± 0.614μm and ChTa 4 1.017 ± 0.417. Also the beads formation was analyzed: ChTa 1 group presented 8 beads, ChTa 2 presented 5, ChTa 3 presented 30 and ChTa 4 presented 15 beads. Conclusion: It can be concluded that it is possible to obtain an effective synthesis of electrospun membranes of PCL and Caesalpinia spinosa extract, indicating a potential of therapeutic application for lesions such as prosthetic stomatitis.KeywordsCandidiasis; Nanofibers; Tannins.

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Production and characterization of polycaprolactone- hyaluronic acid/chitosan- zein electrospun bilayer nanofibrous membrane for tissue regeneration

Cited by 139 publications

References 85 publications

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Nature-Derived Polymers

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Nature-Derived Polymers

Crafting Polymeric and Peptidic Hydrogels for Improved Wound Healing

Synthesis and morphological characterization of Polycaprolactone (PCL) membranes with tara extract (Caesalpinia spinosa)

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