Production and characterization of natural rubber–Ca/P blends for biomedical purposes

Nascimento, Rodney Marcelo do; Faita, Fabrício Luiz; Agostini, Deuber Lincon da Silva; Job, Aldo Eloízo; Guimarães, Francisco Eduardo Gontijo; Bechtold, Ivan H.

doi:10.1016/j.msec.2014.02.019

Cited by 26 publications

(19 citation statements)

References 40 publications

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“…Besides bone grafts, new materials have been reported in the literature with the potential recovery of tissues, for example, natural latex extracted from Hevea brasiliensis [4][5][6][7]. Latex is a substance already described in the literature and has been used extensively in the industry for the production of clinicalsurgical supplies in medical and dental fields, but with few studies on experimental animals.…”

Section: Introductionmentioning

confidence: 99%

Bone repair of critical-sized defects in Wistar rats treated with autogenic, allogenic or xenogenic bone grafts alone or in combination with natural latex fraction F1

Kotake¹,

Gonzaga²,

Coutinho‐Netto³

et al. 2018

Biomed. Mater.

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Bone grafts are used in the medical-surgical field for anatomical and functional reconstruction of lost bone areas, aiding the bone repair process by osteogenesis, osteinduction and osteoconduction. New materials such as F1 (fraction 1) protein extracted from the rubber tree Hevea brasiliensis have been investigated and currently present important properties for tissue repair, and are associated with neoangiogenesis, promoting cell adhesion and extracellular matrix formation. The main objective of this study was to investigate the association of F1 protein to different bone grafts in the repair of critical bone defects in the calvaria of Wistar rats. A total of 112 Wistar rats were divided as follows: autograft (AuG), allograft (AlG), xenograft (XeG), autograft/F1 (AuG-F1), allograft/F1 (AlG-F1), xenograft/F1 (XeG-F1), F1 (F1), control (CTL), with a waiting period of 4 and 6 weeks (w). The stereological AuG, AlG, AuG-F1 and AlG-F1 results had greater bone neoformation (p < 0.05). For immunohistochemistry, the angiogenic and osteogenic factors were higher for AuG-F1 and AlG-F1. TRAP-positive cells were higher in XeG-F1 and AlG (37 ± 9.53, 13.3 ± 4.16) (4 w) and XeG, AlG-F1 and XeG-F1 (20.33 ± 7.37; 15.25 ± 6.02, 19.33 ± 3.21) (6 w). For zymography, F1 showed increased gelatinolytic activity of MMP-2 and -9. It was concluded that the bone graft associated or not with F1 increases the angiogenic and osteogenic, biochemical and stereological factors.

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Section: Introductionmentioning

confidence: 99%

Bone repair of critical-sized defects in Wistar rats treated with autogenic, allogenic or xenogenic bone grafts alone or in combination with natural latex fraction F1

Kotake¹,

Gonzaga²,

Coutinho‐Netto³

et al. 2018

Biomed. Mater.

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“…in the repair of sockets in recently extracted teeth, which observed a minimization of vertical bone loss and that it was well tolerated by patients and helped with healing and maintenance of the dimensions of the alveolar ridge. More recently, Nascimento et al . incorporated Ca/P powder grains produced by sol–gel and Borges et al .…”

Section: Introductionmentioning

confidence: 99%

Application of natural rubber latex as scaffold for osteoblast to guided bone regeneration

Borges

Barros

Garms

et al. 2017

J of Applied Polymer Sci

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Natural rubber latex (NRL) from Hevea brasiliensis is a colloidal system composed of cis-1,4-polyisoprene. Its applications have grown due its angiogenic and wound healing activity. NRL has been used in guided bone regeneration as barrier, enhancing bone formation. However, there has been no study reported so far which shows its in vitro biocompatibility with osteoblasts. Thus, the aim of this work was to apply thermally induced phase separation under several temperatures to induce porosity in NRL; and test its mechanical properties, cytotoxicity, cell adhesion, and mineralization with MC3T3-E1. Only biomembranes submitted at 220 and 210 8C presented porosity. Fourier transform infrared spectroscopy showed no change in cis-1,4-isoprene spectra. Biomembranes were elastic (Young's modulus < 1 MPa). According to ISO10993-5, NRL showed no cytotoxicity. Cells adhered on the NRL and produced mineral matrix as analyzed by scanning electron microscopy-energy-dispersive spectrometry, von kossa, and Fourier transform infrared spectroscopy. Cells on NRL presented higher alkaline phosphatase activity, however, mineralization showed no difference by alizarin red S dye extraction.

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“…When Ca/P precipitation occurs in supersaturated aqueous solutions, a metastable amorphous phase is rapidly formed in the early stages of the reaction. After this formation, it can be converted into the most commonly found crystalline phases: hydroxyapatite (Ca 5 (PO 4 ) 3 (OH)), β-TCP (Ca 3 (PO 4 ) 2 ) and pentacalcium orthophosphate (Ca 5 P 8 ) [47,48]. These precipitates could not be observed in SEM/BSD, being only identifiable through optical microscopy.…”

Section: Evaluation Of Best Sintering Temperaturementioning

confidence: 99%

Production and Characterization of a 316L Stainless Steel/β-TCP Biocomposite Using the Functionally Graded Materials (FGMs) Technique for Dental and Orthopedic Applications

Kuffner

Capellato

Ribeiro

et al. 2021

Metals

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Metallic biomaterials are widely used for implants and dental and orthopedic applications due to their good mechanical properties. Among all these materials, 316L stainless steel has gained special attention, because of its good characteristics as an implantable biomaterial. However, the Young’s modulus of this metal is much higher than that of human bone (~193 GPa compared to 5–30 GPa). Thus, a stress shielding effect can occur, leading the implant to fail. In addition, due to this difference, the bond between implant and surrounding tissue is weak. Already, calcium phosphate ceramics, such as beta-tricalcium phosphate, have shown excellent osteoconductive and osteoinductive properties. However, they present low mechanical strength. For this reason, this study aimed to combine 316L stainless steel with the beta-tricalcium phosphate ceramic (β-TCP), with the objective of improving the steel’s biological performance and the ceramic’s mechanical strength. The 316L stainless steel/β-TCP biocomposites were produced using powder metallurgy and functionally graded materials (FGMs) techniques. Initially, β-TCP was obtained by solid-state reaction using powders of calcium carbonate and calcium phosphate. The forerunner materials were analyzed microstructurally. Pure 316L stainless steel and β-TCP were individually submitted to temperature tests (1000 and 1100 °C) to determine the best condition. Blended compositions used to obtain the FGMs were defined as 20% to 20%. They were homogenized in a high-energy ball mill, uniaxially pressed, sintered and analyzed microstructurally and mechanically. The results indicated that 1100 °C/2 h was the best sintering condition, for both 316L stainless steel and β-TCP. For all individual compositions and the FGM composite, the parameters used for pressing and sintering were appropriate to produce samples with good microstructural and mechanical properties. Wettability and hemocompatibility were also achieved efficiently, with no presence of contaminants. All results indicated that the production of 316L stainless steel/β-TCP FGMs through PM is viable for dental and orthopedic purposes.

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Production and characterization of natural rubber–Ca/P blends for biomedical purposes

Cited by 26 publications

References 40 publications

Bone repair of critical-sized defects in Wistar rats treated with autogenic, allogenic or xenogenic bone grafts alone or in combination with natural latex fraction F1

Bone repair of critical-sized defects in Wistar rats treated with autogenic, allogenic or xenogenic bone grafts alone or in combination with natural latex fraction F1

Application of natural rubber latex as scaffold for osteoblast to guided bone regeneration

Production and Characterization of a 316L Stainless Steel/β-TCP Biocomposite Using the Functionally Graded Materials (FGMs) Technique for Dental and Orthopedic Applications

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