Bone repair of critical-sized defects in Wistar rats treated with autogenic, allogenic or xenogenic bone grafts alone or in combination with natural latex fraction F1

Kotake, Bruna Gabriela dos Santos; Gonzaga, Miliane Gonçalves; Coutinho‐Netto, Joaquim; Ervolino, Edílson; Figueiredo, Fellipe Augusto Tocchini de; Issa, João Paulo Mardegan

doi:10.1088/1748-605x/aa9504

Cited by 18 publications

(7 citation statements)

References 39 publications

(45 reference statements)

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“…The results obtained in this study corroborate with others studys in the literature in which biomaterials of xenogenic origen ewere used in critical defects, and despite showing interaction with the repair process, they did not induce bone neoformation (32). However, he disagrees with the results found by Santos Kotake et al (33) who demonstrated that xenogenous biomaterials can play a positive role to new bone formation. The repair process did not show histological changes from the first to the second observation period, with no newly formed bone tissue and growth of fibrous connective tissue involving the remaining particles.…”

Section: Critical Defectsupporting

confidence: 86%

Biocompatibility and osteopromotor factor of bovine integral bone—a microscopic and histometric analysis

Bassi¹,

Bizelli²,

Consolaro³

et al. 2021

Front Oral Maxillofac Med

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Background: The objective of this study was to evaluate the biological properties, biocompatibility, and osteopromotor factor of integral bone of bovine origin implanted in critical defects of rat calvaria and the subcutaneous plane of rats. Methods:The study was divided into two stages. For the first stage, 24 rats were divided into two groups of 12 animals: group GC, in which the critical defect was filled only by clot, and group GO, in which the defect was filled with particulate biomaterial, and the analysis performed at 30 and 60 days postoperatively.For the second stage, 16 rats were divided into two groups of eight animals: the GOP group, in which the biomaterial in its particulate form was inserted in the subcutaneous plane, and the GOB group, in which the block biomaterial was inserted in the subcutaneous; and the analysis performed at 15 and 45 days postoperatively.Results: The histological and histometric results of the calvaria demonstrated that the biomaterial induced a foreign body reaction over the entire length of the defect and around the particles and was not able to induce bone neoformation. Statistically, no difference was observed for the time, biomaterial, and time × biomaterial parameters (P>0.05). Subcutaneous microscopic examination of the pieces obtained at 15 days showed an inflammatory reaction around the particles of the material with the presence of giant cells and at 45 days, and a reduction in the inflammatory reaction and presence of fibrous connective tissue around the particles was observed. with the presence of giant cells, and for the block biomaterial, connective tissue was present in the trabecular spaces. There was no evidence of ectopic bone formation. Conclusions:The biomaterial, despite being biocompatible, do not promote bone neoformation.

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Section: Critical Defectsupporting

confidence: 86%

Biocompatibility and osteopromotor factor of bovine integral bone—a microscopic and histometric analysis

Bassi¹,

Bizelli²,

Consolaro³

et al. 2021

Front Oral Maxillofac Med

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“…High molecular weight hyaluronic acid combined with bovine xenografts has shown an increase in bone healing when compared to bovine xenografts alone [52]. Moreover, the addition of biomembrane fraction 1 protein, obtained from latex, to bone grafts was able to modulate the expression of extracellular matrix degradative enzymes “in vivo” [53]. Another attempt to enhance bone regeneration has been the implantation of bovine xenografts after soaking with “ Hypericum perforatum” extract leading to an improvement in bone healing [54].…”

Section: Bioceramic Xenografts: Mammalian Originmentioning

confidence: 99%

Current Stage of Marine Ceramic Grafts for 3D Bone Tissue Regeneration

et al. 2019

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Bioceramic scaffolds are crucial in tissue engineering for bone regeneration. They usually provide hierarchical porosity, bioactivity, and mechanical support supplying osteoconductive properties and allowing for 3D cell culture. In the case of age-related diseases such as osteoarthritis and osteoporosis, or other bone alterations as alveolar bone resorption or spinal fractures, functional tissue recovery usually requires the use of grafts. These bone grafts or bone void fillers are usually based on porous calcium phosphate grains which, once disposed into the bone defect, act as scaffolds by incorporating, to their own porosity, the intergranular one. Despite their routine use in traumatology and dental applications, specific graft requirements such as osteoinductivity or balanced dissolution rate are still not completely fulfilled. Marine origin bioceramics research opens the possibility to find new sources of bone grafts given the wide diversity of marine materials still largely unexplored. The interest in this field has also been urged by the limitations of synthetic or mammalian-derived grafts already in use and broadly investigated. The present review covers the current stage of major marine origin bioceramic grafts for bone tissue regeneration and their promising properties. Both products already available on the market and those in preclinical phases are included. To understand their clear contribution to the field, the main clinical requirements and the current available biological-derived ceramic grafts with their advantages and limitations have been collected.

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“…A previous report (Manfrin Arnez et al, 2012) described that bone formation, osseointegration, and stability of tooth implants placed in circumferential bone defects treated with 0.01% F1 presented similar results to those observed in autogenous bone and blood clot. Additionally, a 5‐mm cranial bone defect treated with autogenous and allogenic grafts with or without F1‐protein showed similar bone formation and expression of osteogenic and angiogenic growth factors at 4 and 6 weeks (Santos Kotake et al, 2018). In another study that used the same experimental model, treatment with 5 μg of F1/ monoolein gel showed new bone tissue formation at 2 and 6 weeks; however, the bone growth was significantly lower when compared to 5 μg of BMP‐2/ monoolein gel treatment (Issa et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Concentration‐dependent effects of latex F1‐protein fraction incorporated into deproteinized bovine bone and biphasic calcium phosphate on the repair of critical‐size bone defects

et al. 2020

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F1-protein fraction (F1) is a natural bioactive compound extracted from the rubber tree, Hevea brasiliensis, and has been recently studied for its therapeutic potential in wound healing. In this study, we investigated the concentration-dependent effects of F1 (0.01%, 0.025%, 0.05%, and 0.1%) incorporated into deproteinized bovine bone (DBB) and porous biphasic calcium phosphate (pBCP), on the repair of rat calvarial critical-size bone defects (CSBD). The defects were analyzed by 3Dmicrotomography and 2D-histomorphometry at 12 weeks postsurgery. The binding efficiency of F1 to pBCP (96.3 ± 1.4%) was higher than that to DBB (67.7 ± 3.3%). In vivo analysis showed a higher bone volume (BV) gain in all defects treated with DBB (except in 0.1% of F1) and pBCP (except in 0.05% and 0.1% of F1) compared to the CSBD without treatment/control group (9.96 ± 2.8 mm 3). DBB plus 0.025% F1 promoted the highest BV gain (29.7 ± 2.2 mm 3 , p < .0001) compared to DBB without F1 and DBB plus 0.01% and 0.1% of F1. In the pBCP group, incorporation of F1 did not promote bone gain when compared to pBCP without F1 (15.9 ± 4.2 mm 3 , p > .05). Additionally, a small BV occurred in defects treated with pBCP plus 0.1% F1 (10.4 ± 1.4 mm 3, p < .05). In conclusion, F1 showed a higher bone formation potential in combination with DBB than with pBCP, in a concentration-dependent manner. Incorporation of 0.25% F1 into DBB showed the best results with respect to bone formation/repair in CSBD. These results suggest that DBB plus 0.25% F1 can be used as a promising bioactive material for application in bone tissue engineering. K E Y W O R D S bone repair, deproteinized bovine bone, natural latex protein fraction, porous biphasic calcium phosphate, protein adsorption, rat calvarial critical-size bone defects 1 | INTRODUCTION Bone defects can heal spontaneously under suitable physiological and environmental conditions due to the bone's ability to repair (Cameron et al., 2013). However, large skeletal defects resulting from malformation, infection, trauma, or tumor resection are challenging problems in contemporary reconstructive surgery because the size of these defects exceed the bone's natural capacity to repair (Henkel et al.,

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Bone repair of critical-sized defects in Wistar rats treated with autogenic, allogenic or xenogenic bone grafts alone or in combination with natural latex fraction F1

Cited by 18 publications

References 39 publications

Biocompatibility and osteopromotor factor of bovine integral bone—a microscopic and histometric analysis

Biocompatibility and osteopromotor factor of bovine integral bone—a microscopic and histometric analysis

Current Stage of Marine Ceramic Grafts for 3D Bone Tissue Regeneration

Concentration‐dependent effects of latex F1‐protein fraction incorporated into deproteinized bovine bone and biphasic calcium phosphate on the repair of critical‐size bone defects

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