Production and characterization of monoclonal antibodies against tissue specific epitopes on ABO blood group substances in saliva

Kimura, Akihiko; Matsumura, Fuseo; Sodesaki, Ken-ichiro; Tsuji, Tsutomu

doi:10.1007/bf01369805

Cited by 10 publications

(10 citation statements)

References 9 publications

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“…Some polyclonal antisera specific for semen (Tsuda et al 1985), saliva (Eckersall et al 1981), sweat (Tokiwa et al 1987) and vaginal secretions (Itoh 1990) have been developed, and a few of them are used in practice. ABH antigens of body fluids were expected to be distributed on particular proteins (Sagisaka 1989;Sagisaka et al 1989Sagisaka et al , 1990, therefore monoclonal antibodies against the specific "carrier" proteins of saliva (Kimura et al 1991a) and semen (Kimura et al 1991b; Kishida et al 1991) have been also prepared for blood grouping. We established a hybridoma clone excreting an IgGI monoclonal antibody specific for the amniotic fluid.…”

Section: Discussionmentioning

confidence: 99%

Monoclonal Antibody against an Amniotic Protein Carrying ABH Blood Group Epitopes and Its Forensic Application.

Nagae

Aoki

Nata

et al. 1995

Tohoku J. Exp. Med.

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A mouse monoclonal antibody against an amniotic protein carrying ABH antigenic epitopes was established. BALB/c mice were immunized by an amniotic protein of molecular weight over 200 kDa, which had proved to be the carrier protein of ABH blood group epitopes by analysis with SDS-PAGE and immunoblotting. The antibody, ASP-1, was directed to the amniotic carrier protein without affecting the ABH blood group antigenicity, and did not cross-react with other body fluids which included blood, saliva, semen, urine or vaginal secretion. The immunoglobulin class of ASP-1 was IgGI with a titer of 1:1,600. ASP-1 was used to detect the ABH blood group of amniotic fluid by the sandwich ELISA in which wells of plates were coated with ASP-1, and the ABH blood group of the captured protein was detected with mouse IgM anti-A and -B antibodies and enzyme conjugated anti-mouse IgM. The sandwich ELISA could successfully detect the blood group of amniotic fluid in mixed body fluids. amniotic fluid; ABH blood group; ELISA; monoclonal antibody; forensic serologyThe identification of pregnancy from evidence found at a scene may give information for criminal investigations in cases of neonaticide. As a hallmark of the delivery, some pregnancy associated proteins have been studied (Sagisaka and Takahashi 1975), whereas attempts to detect the amniotic fluid from the scene are less common. It has been confirmed that the ABH blood group antigen in amniotic fluid are derived from the fetus in and after mid-trimester (Ogita et al. 1975). The amniotic protein carrying the ABH blood group epitopes, however, has not been identified. The evidence originating from amniotic fluid should co-exist with that of other body fluids such as blood, urine and vaginal secretions with few exceptions. Thus, ABO blood grouping of each constituent of a mixed body fluid should be mandatory for the scene investigation in cases of neonaticide. In this study, we analysed an amniotic protein carrying the ABH blood group antigens and established a monoclonal antibody against it. The potential avail-

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Section: Discussionmentioning

confidence: 99%

Monoclonal Antibody against an Amniotic Protein Carrying ABH Blood Group Epitopes and Its Forensic Application.

Nagae

Aoki

Nata

et al. 1995

Tohoku J. Exp. Med.

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“…These mAbs recognized the core protein of salival BGS and were tissue and species specific [1], and could be employed for blood grouping of A. Kimura et al: ABO from mixed body fluids saliva from mixed body fluids [2]. We think that the sandwich method with mAb against TSE of BGS may be the universal method for ABO blood grouping of individual body fluids from a mixture of body fluids.…”

Section: Blood Grouping Of Semen By Sandwich Methods With P6-5hmentioning

confidence: 98%

“…The ABO blood group epitope in the eluate was detected to a 104-fold dilution by ELISA and this eluate was used as the immunogen. Immunization of mice with immunogen, fusion of mice lymph nodes cells with myeloma cells (P3U1) and establishment of antibody-producing hybridomas were performed essentially as described in the previous paper [1].…”

Section: Methodsmentioning

confidence: 99%

“…ELISA was performed as described in the previous paper [1], except that biotinconjugated F(ab')2 fraction of sheep anti-mouse IgG(X2, 500 dil. ; Cappel, Pa, USA) was used as secondary antibody.…”

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

“…Recently, we developed methods for ABO blood grouping of saliva from mixed body fluids by sandwich methods using monoclonal antibodies (mAbs) to a tissue-specific epitope (TSE) on the core protein of an ABO blood group substance (BGS) in saliva [1,2]. If seminal ABO-BGS also carries TSE on its core protein, ABO blood grouping of semen from mixed body fluids can be performed by sandwich methods using mAb to TSE of seminal ABO-BGS.…”

Section: Introductionmentioning

confidence: 99%

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ABO blood grouping of semen from mixed body fluids with monoclonal antibody to tissue-specific epitopes on seminal ABO blood group substance

et al. 1991

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Practical methods were developed for ABO blood grouping of semen from mixed body fluids. An monoclonal antibody (P6-5H) which recognizes a tissue-specific epitope on a seminal ABO blood group substance (alpha 2-seminoglycoprotein) was used as a solid phase antibody for selective capture of the seminal ABO blood group substance. ABO blood group epitopes of secretor and non-secretor semen were detected in dilutions of 8 x 10(3)-3.2 x 10(4) and 8 x 10(3)-fold, respectively, by sandwich ELISA. ABO blood group epitopes were also detected in dilutions up to 4 x 10(3)-fold, irrespective of secretor status, by the sandwich absorption-elution test.

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Immunohistochemical study on the localization of the epitope defined by a human saliva-specific mouse monoclonal antibody (P4-5C)

et al. 1991

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A novel mouse monoclonal antibody (P4-5C) has been developed which recognizes the core portion of the protein carrying ABO(H) blood group antigens in human saliva. This proved to be specific for human saliva using immunochemical investigations such as enzyme-linked immunosorbent assay, Ouchterlony method and counter-immunoelectrophoresis. By light and electron microscope studies with immunohistochemical techniques using this human saliva-specific P4-5C as primary antibody, it was shown that P4-5C reacted specifically and exclusively with mucus from the mucous gland cells of human salivary glands. P4-5C reacted neither with the mucous gland cells of other primates (hamadryas baboon, Japanese monkey and Rhesus monkey) and four mammals (dog, cat, rabbit and mouse) nor with other human tissues. The epitope on the core portion of the ABO(H)-carrying protein was defined by P4-5C and could be discriminated from the epitope of ABO(H) blood group antigens using immunoelectronmicroscopy, although these 2 epitopes were localized relatively close to each other. The P4-5C monoclonal antibody can be also used for morphological species identification of tissue specimens from submandibular glands.

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Production and characterization of monoclonal antibodies against tissue specific epitopes on ABO blood group substances in saliva

Cited by 10 publications

References 9 publications

Monoclonal Antibody against an Amniotic Protein Carrying ABH Blood Group Epitopes and Its Forensic Application.

Monoclonal Antibody against an Amniotic Protein Carrying ABH Blood Group Epitopes and Its Forensic Application.

ABO blood grouping of semen from mixed body fluids with monoclonal antibody to tissue-specific epitopes on seminal ABO blood group substance

Immunohistochemical study on the localization of the epitope defined by a human saliva-specific mouse monoclonal antibody (P4-5C)

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