ABO blood grouping of semen from mixed body fluids with monoclonal antibody to tissue-specific epitopes on seminal ABO blood group substance

Kimura, Akihiko; Matsumura, Fuseo; Sodesaki, Ken-ichiro; Osawa, Masatoshi; Ikeda, Hiroaki; Yasuda, Seiji; Tsuji, Tsutomu

doi:10.1007/bf01369580

Cited by 8 publications

(2 citation statements)

References 6 publications

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“…For this purpose, histological or immunological procedures have been mainly employed. Monoclonal antibodies (mAbs) specific for marker proteins or carbohydrate chains are suitable reagents for this purpose [2,3].…”

Section: Introductionmentioning

confidence: 99%

Brain tissue identification based on myosin heavy chain isoforms

et al. 1995

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Non-muscle tissues contain 3 myosin heavy chain (MHC) isoforms; MIIA, MIIB1 and MIIB2. MIIA is a non-muscle type isoform distributed in all non-muscle tissues and smooth-muscle, while MIIB1 and MIIB2 are brain-type isoforms distributed mainly in neuronal tissues. The ratio of MIIA and MIIB (A/B ratio) differs between tissues, suggesting that this ratio may be a useful marker for tissue identification. To apply the A/B ratio for tissue identification in forensic practice, we developed a highly sensitive ELISA for quantification of each MHC isoform. At least 100 pg of both MHC isoforms could be detected by the present method. Analysis of the A/B ratio of the cerebrum, cerebellum, liver, kidney, spleen and andrenal gland by the present method indicated that the A/B ratio of the brain tissue (< 0.5) was quite different from other tissues (> 3.0). The A/B ratio could be determined from at least 8 micrograms of fresh tissue sample and 0.1 mg of dried tissue sample stored for 1 month at room temperature. Therefore, the A/B ratio seems to be an excellent marker for identification of the brain tissue.

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Section: Introductionmentioning

confidence: 99%

Brain tissue identification based on myosin heavy chain isoforms

et al. 1995

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“…Some polyclonal antisera specific for semen (Tsuda et al 1985), saliva (Eckersall et al 1981), sweat (Tokiwa et al 1987) and vaginal secretions (Itoh 1990) have been developed, and a few of them are used in practice. ABH antigens of body fluids were expected to be distributed on particular proteins (Sagisaka 1989;Sagisaka et al 1989Sagisaka et al , 1990, therefore monoclonal antibodies against the specific "carrier" proteins of saliva (Kimura et al 1991a) and semen (Kimura et al 1991b; Kishida et al 1991) have been also prepared for blood grouping. We established a hybridoma clone excreting an IgGI monoclonal antibody specific for the amniotic fluid.…”

Section: Discussionmentioning

confidence: 99%

Monoclonal Antibody against an Amniotic Protein Carrying ABH Blood Group Epitopes and Its Forensic Application.

Nagae

Aoki

Nata

et al. 1995

Tohoku J. Exp. Med.

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A mouse monoclonal antibody against an amniotic protein carrying ABH antigenic epitopes was established. BALB/c mice were immunized by an amniotic protein of molecular weight over 200 kDa, which had proved to be the carrier protein of ABH blood group epitopes by analysis with SDS-PAGE and immunoblotting. The antibody, ASP-1, was directed to the amniotic carrier protein without affecting the ABH blood group antigenicity, and did not cross-react with other body fluids which included blood, saliva, semen, urine or vaginal secretion. The immunoglobulin class of ASP-1 was IgGI with a titer of 1:1,600. ASP-1 was used to detect the ABH blood group of amniotic fluid by the sandwich ELISA in which wells of plates were coated with ASP-1, and the ABH blood group of the captured protein was detected with mouse IgM anti-A and -B antibodies and enzyme conjugated anti-mouse IgM. The sandwich ELISA could successfully detect the blood group of amniotic fluid in mixed body fluids. amniotic fluid; ABH blood group; ELISA; monoclonal antibody; forensic serologyThe identification of pregnancy from evidence found at a scene may give information for criminal investigations in cases of neonaticide. As a hallmark of the delivery, some pregnancy associated proteins have been studied (Sagisaka and Takahashi 1975), whereas attempts to detect the amniotic fluid from the scene are less common. It has been confirmed that the ABH blood group antigen in amniotic fluid are derived from the fetus in and after mid-trimester (Ogita et al. 1975). The amniotic protein carrying the ABH blood group epitopes, however, has not been identified. The evidence originating from amniotic fluid should co-exist with that of other body fluids such as blood, urine and vaginal secretions with few exceptions. Thus, ABO blood grouping of each constituent of a mixed body fluid should be mandatory for the scene investigation in cases of neonaticide. In this study, we analysed an amniotic protein carrying the ABH blood group antigens and established a monoclonal antibody against it. The potential avail-

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Origin of an 84‐kDa Protein with ABH Blood‐Typing Antigen Activity in Human Seminal Plasma

SATO,

NAKAMURA,

YAMAZAKI

et al. 1999

Journal of Andrology

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In order to investigate the origin of an 84‐kDa protein with ABH blood‐typing antigen activity (p 84) and its concentration in human seminal plasma, a monoclonal antibody (mAb p 84) was produced. The protein was recognized in breast milk as well as in seminal plasma by an indirect, enzyme‐linked immunosorbent assay (ELISA) using this mAb. mAb p 84 identified 84‐kDa and 83‐kDa forms of the protein in seminal plasma and breast milk, respectively, on immunoblotting. The mean concentration of p 84 in seminal plasma was 949 μg/ml (n = 54 subjects). There was no significant difference in the concentration of p 84 between individuals who secreted (Se) or did not secrete (non‐se) the ABH antigen into their seminal plasma, nor were there any significant correlations between the concentration of p 84 and the total seminal protein concentration. An immunohistochemical study using mAb p 84 with light microscopic detection showed that p 84 was located in the cytoplasm of the inner layer of pseudostratified cuboidal epithelial cells of the seminal vesicles, but no immunoreactivity was found in the prostate. These data indicate that p 84 originates from a single tissue, the seminal vesicles, and suggest that p 84 is an ABH epitope‐bearing protein that has not previously been identified but possesses some immunological properties similar to those of lactotransferrin.

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ABO blood grouping of semen from mixed body fluids with monoclonal antibody to tissue-specific epitopes on seminal ABO blood group substance

Cited by 8 publications

References 6 publications

Brain tissue identification based on myosin heavy chain isoforms

Brain tissue identification based on myosin heavy chain isoforms

Monoclonal Antibody against an Amniotic Protein Carrying ABH Blood Group Epitopes and Its Forensic Application.

Origin of an 84‐kDa Protein with ABH Blood‐Typing Antigen Activity in Human Seminal Plasma

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