Production and characterization of monoclonal antibodies to four Egtved virus structural proteins

Lorenzen, Niels; Olesen, N. J.; Jorgensen, P Vestergård

doi:10.3354/dao004035

Cited by 90 publications

(108 citation statements)

References 17 publications

Supporting

Mentioning

104

Contrasting

Order By: Relevance

“…MAbs have been developed against a variety of fish viruses, including rhabdoviruses (Schultz et al, 1985;Lorenzen et al, 1988;Kamei et al, 1990;Kasornchandra et al, 1992), birnaviruses (CaswellReno et al, 1986;Lipipun et al, 1989;Kamei et al, 1990;Chi et al, 1991) and herpesviruses (Arkush et A B al., 1992;Hayashi et al, 1993). In the present study, we describe the production of the MAbs against a piscine iridovirus, RSIV.…”

Section: Discussionmentioning

confidence: 99%

Production of Monoclonal Antibodies against Red Sea Bream Iridovirus.

Nakajima¹,

Sorimachi²

1995

Fish Pathol.

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Production of Monoclonal Antibodies against Red Sea Bream Iridovirus.

Nakajima¹,

Sorimachi²

1995

Fish Pathol.

View full text Add to dashboard Cite

“…SDS-PAGE was performed according to the method of Laemmli (1970) using 10% (w/v) acrylamide gels under reducing conditions. Western blotting was performed according to the principles described by Lorenzen et al (1988). Polypeptides that were separated by SDS-PAGE were electroblotted onto a nitrocellulose membrane in a semi-dry electroblotter (Bio-Rad).…”

Section: Methodsmentioning

confidence: 99%

“…The subsequent procedure was the same as described previously. MAb IP5B11 (Lorenzen et al 1988) was used as a positive control.…”

Section: Methodsmentioning

confidence: 99%

“…The reactivity of selected MAbs against a panel of VHSV (Table 2) was evaluated using doublesandwich ELISA as described by Olesen & Jørgensen (1991), except that a mixture of protein-A purified rabbit anti G-, N-and P-protein of VHSV was used as a first layer. As a positive control, MAb IP5B11 against VHSV N-protein (Lorenzen et al 1988) was used. This MAb has been proven to react with all known VHSV isolates (tested against >1000 isolates, N. J. Olesen pers.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Development of a monoclonal antibody against viral haemorrhagic septicaemia virus (VHSV) genotype IVa

Ito¹,

Olesen²,

Skall³

et al. 2010

Dis. Aquat. Org.

Self Cite

View full text Add to dashboard Cite

The viral haemorrhagic septicaemia virus (VHSV) comprises 4 major genotypes and a number of subtypes with, in most cases, distinct geographical distribution. A quick and simple detection method that can discriminate the different genotypes is desirable for a quick and more efficient prevention of the spread of genotypes to new geographical areas. A monoclonal antibody (MAb) against VHSV genotype IVa was produced, with the aim of providing a simple method of discriminating this genotype from the other VHSV genotypes (I, II, III and IVb). Balb/c mice were injected with purified VHSV-JF00Ehil (genotype IVa) from diseased farmed Japanese flounder. Ten hybridoma clones secreting monoclonal antibodies (MAbs) against VHSV were established. One of these, MAb VHS-10, reacted only with genotype IVa in indirect fluorescent antibody technique (IFAT) and ELISA. Using cell cultures that were transfected with each of the viral protein genes, it was shown that the MAb VHS-10 recognizes a nonlinear genotype IVa-specific epitope on the VHSV N-protein.

show abstract

“…A monoclonal antibody (MAb) against VHSV N protein (IP5B11; Lorenzen et al, 1988) provided by Dr. N. J. Olesen (Danish Veterinary Labora tory) was applied as the primary antibody at a dilution of 1 : 100 and a FITC-conjugated rabbit antiserum to mouse lg (1:40, DAKO) was applied as the secondary antibody. …”

Section: Indirect Immuno-fluorescent Antibody Technique (Ifat)mentioning

confidence: 99%

Isolation of Viruses from Wild Japanese Flounder Paralichthys olivaceus.

Takano¹,

Mori²,

Nishizawa³

et al. 2001

Fish Pathol.

View full text Add to dashboard Cite

ABSTRACT-For a survey on fish pathogenic viruses among wild Japanese flounder Paralichthys olivaceus, 274 fish (body weight: 29-2240 g) were collected in 9 coastal areas of Japan in 1999 and 2000. Aquabirnaviruses were isolated, using RTG-2 and/or SSN-1 cells, from 111 fish (40.5%) caught in almost all the coastal areas and the representative isolate was identified as YTAV (yellow tail ascites virus) by cross-neutralization tests.Besides aquabirnaviruses, VHSV (viral hemor rhagic septicemia virus) was isolated, using EPC, SSN-1 and/or FHM cells, from 18 fish (6.6%) collected in 2 areas mainly in winter and spring. In a pathogenicity test, 4 isolates of aqua birnaviruses did not cause any mortality or morbidity in young Japanese flounder (average body weight 8.7 g) by intramuscular injection at 103.0-107.6 TCID50/ fish. Inoculation of an isolate of VHSV at 102.0 and 106.0TCID50 /fish produced mortalities of 60% and 100%, respectively, in flounder (24.8 g). Common external signs of flounder experimentally infected with VHSV were dark colora tion of the body with hemorrhagic fins and abdominal swelling, and the internal signs included ascites and extensive hemorrhage in the muscle and/or viscera.

show abstract

Production and characterization of monoclonal antibodies to four Egtved virus structural proteins

Cited by 90 publications

References 17 publications

Production of Monoclonal Antibodies against Red Sea Bream Iridovirus.

Production of Monoclonal Antibodies against Red Sea Bream Iridovirus.

Development of a monoclonal antibody against viral haemorrhagic septicaemia virus (VHSV) genotype IVa

Isolation of Viruses from Wild Japanese Flounder Paralichthys olivaceus.

Contact Info

Product

Resources

About