Production and characterization of cellulases and hemicellulases by Acremonium cellulolyticus using rice straw subjected to various pretreatments as the carbon source

Hideno, Akihiro; Inoue, Hiroyuki; Tsukahara, Kenichiro; Yano, Shinichi; Xu, Fang; Endo, Takashi; Sawayama, Shigeki

doi:10.1016/j.enzmictec.2010.10.005

Cited by 69 publications

(26 citation statements)

References 27 publications

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“…4). The on-site production or self-preparation of cellulases has been proposed as a cost-effective method (Fujimoto et al, 2008;Hideno et al, 2012). The glucose yields of Shiozuka and M×G obtained using the enzyme from a culture of T.…”

Section: Enzymatic Hydrolysis Of Ahp-treatedmentioning

confidence: 99%

“…The costs of cellulases including the purchase cost of commercial cellulases or their production cost, contribute to a large proportion of the total costs of biorefinery process such as the bioethanol production (Fujimoto et al, 2008). On-site production of cellulases is considered to be a solution for reducing the cost of cellulases, including the purchase cost of and transportation costs for distribution (Fujimoto et al, 2008;Hideno et al, 2012). Trichoderma reesei and its mutant strains have been widely investigated (Zhang et al, 2006) as promising hyper cellulase-producing fungi.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of the enzymatic digestibility of physically and chemically pretreated selected line of diploid- Miscanthus sinensis Shiozuka and triploid- M. × giganteus

Hideno

Kawashima

Anzoua

et al. 2013

Bioresource Technology

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The diploid Miscanthus sinensis "Shiozuka" which was selected as a high-biomass producing line, and the triploid M. × giganteus (M×G) were treated by ball milling (physical treatment) and alkaline hydrogen peroxide treatment (AHP; chemical treatment), and their structural sugar compositions and enzymatic digestibility were compared. The structural sugar content of Shiozuka was moderate and lower than that of M×G. The Klason lignin content of Shiozuka was also lower than that of M×G.However, Shiozuka was sensitive to ball milling and AHP treatment; ball milled and AHP-treated Shiozuka had higher enzymatic digestibility than ball milled and AHPtreated M×G. Shiozuka would be promising feedstock to obtain fermentable sugars with low energy consumption. Finally, enzymes for the hydrolysis of chemically treated Miscanthus were isolated from Trichoderma reesei ATCC 66589 and Penicillium pinophilum. The sugar yield could be increased by enzymatic hydrolysis of AHP-treated samples with NaOH and H 2 O 2 and the isolated enzymes. Highlights> Miscanthus sinensis "Shiozuka" contained moderate sugar but low lignin contents.> Shiozuka was more sensitive to physical and chemical treatments than M×G. >Enzymatic digestibilities of the pretreated Shiozuka were higher than those of M×G.>Shiozuka is a promising bioenergy grass cultivar.3

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Section: Enzymatic Hydrolysis Of Ahp-treatedmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of the enzymatic digestibility of physically and chemically pretreated selected line of diploid- Miscanthus sinensis Shiozuka and triploid- M. × giganteus

Hideno

Kawashima

Anzoua

et al. 2013

Bioresource Technology

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“…볏짚은 쌀 이 주식인 나라에서 생산되는 주요 농업부산물이며, 전세계적 으로 연간 731,000,000톤이 생산되며 [16], 이 양은 205 GL의 바이오에탄올로 전환될 수 있다 [7]. 섬유성 바이오메스를 구 성하는 식물고분자 탄수화물은 주로 cellulose와 hemicellulose 및 페놀계 중합체인 리그닌으로 구성되어 있다 [11].…”

Section: 서 론unclassified

Optimum Enzyme Mixture of Cellulase, Hemicellulase, and Xylanase for Production of Water-Soluble Carbohydrates from Rice Straw

Cho¹,

Lee²,

Kim³

et al. 2012

Journal of Life Science

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This study was conducted to investigate the production of water-soluble carbohydrates (WSCs) by treatment of different amounts of rice straw with cellulase, hemicellulase, and xylanase. Treatment of high amounts of rice straw (100 g/l) with cellulase and hemicellulase resulted in similar production of WSCs. Reducing the amount of rice straw to 50 g/l decreased the production of WSCs by hemicellulase but had no effect on WSC production by cellulase. The interaction among rice straw amounts, and hemicellulase and xylanase activities was investigated using a Box Behnken design and a response surface model. An interaction was found only between hemicellulase and xylanase. An enzyme mixture consisting of 0.55 mg/ml of hemicellulase and 0.65 mg/ml of xylanase generated the highest amounts of WSCs, regardless of the amount of rice straw provided. Therefore, the activity of cellulase was higher than that of either hemicellulose or xylanase for WSC production from rice straw. The interaction observed for hemicellulase and xylanase indicates that a combined enzyme treatment could improve the production of WSCs from rice straw.

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“…최근에는 cellulose와 xylan으로부터 기능성 올리고당과 같은 기능성 물질의 생산 뿐만 아니라 차세대 대 체 에너지로서 많은 각광을 받고 있는 바이오 에탄올을 생산 하기 위한 원료로서 cellulose와 xylan을 활용하기 위한 연구 가 높은 관심속에서 수행되어지고 있다 [4,17,19,22,27]. 이러한 관점에서 cellulose와 xylan의 유용한 활용을 위해서는 먼저 이들 biomass 자원의 구성 단당인 glucose와 xylose로의 분해 가 선행되어야 하는데 지구 환경오염의 문제가 현대 사회에 심각하게 대두되면서 화학적인 분해 방법을 대신하여 환경적 으로 온화한 미생물 유래의 효소를 이용하여 cellulose와 xylan을 분해하는 생물학적 방법이 많은 주목을 받게 되었다 [6,7,9,12]. Cellulose는 식물세포벽의 주성분으로서 d-glucose 가 β-1,4결합으로 연결되어 있는 d-glucose 중합체로서 endo-β-1,4-cellulase (EC 3.2.1.4), exo-β-1,4-cellulase (EC 3.2.1.91) 그리고 β-1,4-glucosidase (EC 3.2.1.21)의 협동적인 상승작용 에 의하여 구성 단당인 d-glucose로 분해가 이루어진다 [1,5,15].…”

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Characterization of Cellulase and Xylanase from Bacillus subtilis NC1 Isolated from Environmental Soil and Determination of Its Genes

Park¹,

Kang²,

Choi³

2012

Journal of Life Science

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A Bacillus sp. strain producing celluase and xylanase was isolated from environmental soil with LB agar plate containing carboxymethylcellulose (CM-cellulose) and beechwood xylan stained with trypan blue as substrates, respectively. Based on the 16S rRNA gene sequence and API 50 CHL test, the strain was identified as B. subtilis and named B. subtilis NC1. The cellulase and xylanase from B. subtilis NC1 exhibited the highest activities for CM-cellulose and beechwood xylan as substrate, respectively, and both enzymes showed the maximum activity at pH 5.0 and 50°C. We cloned and sequenced the genes for cellulase and xylanase from genomic DNA of the B. subtilis NC1 by the shot-gun cloning method. The cloned cellulase and xylanase genes consisted of a 1,500 bp open reading frame (ORF) encoding a 499 amino acid protein with a calculated molecular mass of 55,251 Da and a 1,269 bp ORF encoding a 422 amino acid protein with a calculated molecular mass of 47,423 Da, respectively. The deduced amino acid sequences from the genes of cellulase and xylanase showed high identity with glycosyl hydrolases family (GH) 5 and 30, respectively. [10,14,20,23,25]. 최근에는 cellulose와 xylan으로부터 기능성 올리고당과 같은 기능성 물질의 생산 뿐만 아니라 차세대 대 체 에너지로서 많은 각광을 받고 있는 바이오 에탄올을 생산 하기 위한 원료로서 cellulose와 xylan을 활용하기 위한 연구 가 높은 관심속에서 수행되어지고 있다 [4,17,19,22,27]. 이러한 관점에서 cellulose와 xylan의 유용한 활용을 위해서는 먼저 이들 biomass 자원의 구성 단당인 glucose와 xylose로의 분해 가 선행되어야 하는데 지구 환경오염의 문제가 현대 사회에 심각하게 대두되면서 화학적인 분해 방법을 대신하여 환경적 으로 온화한 미생물 유래의 효소를 이용하여 cellulose와 xylan을 분해하는 생물학적 방법이 많은 주목을 받게 되었다 [6,7,9,12]. Cellulose는 식물세포벽의 주성분으로서 d-glucose 가 β-1,4결합으로 연결되어 있는 d-glucose 중합체로서 endo-β-1,4-cellulase (EC 3.2.1.4), exo-β-1,4-cellulase (EC 3.2.1.91) 그리고 β-1,4-glucosidase (EC 3.2.1.21)의 협동적인 상승작용 에 의하여 구성 단당인 d-glucose로 분해가 이루어진다 [1,5,15]. 그리고, xylan은 식물 세포벽 성분 중에서 cellulose 다음으로 풍부하게 존재하는 hemicellulose의 주성분으로서 d-xylose가 β-1,4 결합으로 연결되어 있는 중합체이며 endo-β -1,4-xylanase (EC 3.2.1.8)와 β-1,4-xylosidase (EC 3.2.1.37)의

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Production and characterization of cellulases and hemicellulases by Acremonium cellulolyticus using rice straw subjected to various pretreatments as the carbon source

Cited by 69 publications

References 27 publications

Comparison of the enzymatic digestibility of physically and chemically pretreated selected line of diploid- Miscanthus sinensis Shiozuka and triploid- M. × giganteus

Comparison of the enzymatic digestibility of physically and chemically pretreated selected line of diploid- Miscanthus sinensis Shiozuka and triploid- M. × giganteus

Optimum Enzyme Mixture of Cellulase, Hemicellulase, and Xylanase for Production of Water-Soluble Carbohydrates from Rice Straw

Characterization of Cellulase and Xylanase from Bacillus subtilis NC1 Isolated from Environmental Soil and Determination of Its Genes

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