Production and Characterization of an Integrated Multi-Layer 3D Printed PLGA/GelMA Scaffold Aimed for Bile Duct Restoration and Detection

Xiang, Yu‐Tao; Wang, Weijia; Gao, Yuanhui; Zhang, Jianquan; Zhang, Jing; Bai, Zhiming; Zhang, Shufang; Yang, Yijun

doi:10.3389/fbioe.2020.00971

Cited by 13 publications

(5 citation statements)

References 46 publications

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“…The FTIR spectrum of GelMA exhibits a sharp peak at 1630 cm −1 , corresponding to C=O stretching groups of amide I bond (labelled as yellow) (Farasatkia , 2021). The peak at 1546 cm −1 is attributed to the N-H bending groups of the amide II bond (labelled as green); the peak at 1244 cm −1 is related to the C-N stretching and N-H bending of the amide III bond (labelled as pink) (Xiang et al, 2020;Farasatkia et al, 2021). Furthermore, the peak located in the range of 3200-3400 cm −1 (labelled as blue) detected in GelMA is associated with the existence of peptide bonds (N-H stretching) and -OH functional groups (Sreekumaran et al, 2021).…”

Section: Fourier Transform Infrared Spectroscopy (Ftir)mentioning

confidence: 99%

Fabrication and optimization of 3D printed gelatin methacryloyl microneedle arrays based on vat photopolymerization

Baykara

Bedir

Ilhan

et al. 2023

Front. Bioeng. Biotechnol.

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Microneedles (MNs) are micrometer-sized arrays that can penetrate the skin in a minimally invasive manner; these devices offer tremendous potential for the transdermal delivery of therapeutic molecules. Although there are many conventional techniques for manufacturing MNs, most of them are complicated and can only fabricate MNs with specific geometries, which restricts the ability to adjust the performance of the MNs. Herein, we present the fabrication of gelatin methacryloyl (GelMA) MN arrays using the vat photopolymerization 3D printing technique. This technique allows for the fabrication of high-resolution and smooth surface MNs with desired geometries. The existence of methacryloyl groups bonded to the GelMA was verified by 1H NMR and FTIR analysis. To examine the effects of varying needle heights (1000, 750, and 500 µm) and exposure times (30, 50, and 70 s) on GelMA MNs, the height, tip radius, and angle of the needles were measured; their morphological and mechanical properties were also characterized. It was observed that as the exposure time increased, the height of the MNs increased; moreover, sharper tips were obtained and tip angles decreased. In addition, GelMA MNs exhibited good mechanical performance with no breakage up to 0.3 mm displacement. These results indicate that 3D printed GelMA MNs have great potential for transdermal delivery of various therapeutics.

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Section: Fourier Transform Infrared Spectroscopy (Ftir)mentioning

confidence: 99%

Fabrication and optimization of 3D printed gelatin methacryloyl microneedle arrays based on vat photopolymerization

Baykara

Bedir

Ilhan

et al. 2023

Front. Bioeng. Biotechnol.

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“…This makes it a suitable candidate for applications as a coating for bone implants. [47,48] It is also crucial that the bone implants coating remains intact and functional throughout surgery. An antibacterial coating should retain its effectiveness over a longer time period, producing the desired therapeutic effects.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial CATH‐2 Peptide Coating to Prevent Bone Implant‐Related Infection

Keikhosravani

Jahanmard

Bollen

et al. 2023

Adv Materials Technologies

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The development of antibacterial coatings is a promising approach to preventing biofilm formation and reducing the overuse of systemic antibiotics. However, widespread antibiotic use has resulted in antibiotic‐resistant bacteria, limiting the efficacy of antibiotic‐based coatings. Herein, an antibacterial coating is developed by layer‐by‐layer (LbL) assembly of two polymers namely PDLG (poly (D,L‐lactide‐co‐glycolide)) and gelatin methacryloyl (GelMA) while chicken cathelicidin‐2 (CATH‐2), a cationic and amphipathic peptide, is loaded between these polymer layers. The electrospray method is used to apply the coatings to achieve efficient peptide loading and durability. The CATH‐2 bactericidal concentration ranges are first identified, followed by a study of their cytotoxicity to human mesenchymal stem cells (hMSCs) and macrophage cell lines. Later, different LbL electrospray coating assemblies loaded with the optimal peptide concentration are sought. Various coating strategies are investigated to identify an LbL coating that exhibits prolonged and biocompatible CATH‐2 release. The resulting CATH‐2‐coated titanium surfaces exhibit strong antibacterial activity against both Staphylococcus aureus and Escherichia coli bacteria for 4 days and are biocompatible with hMSCs and macrophage cells. This coating can be considered as a versatile delivery system platform for the delivery of CATH‐2 peptides while avoiding cytotoxicity, particularly for the prevention of infections associated with implants.

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“…They created a device consisting of a scaffold and mesenchymal stem cells overexpressing basic fibroblast growth factor. They have provided evidence of the device’s potential usage in regenerating full-thickness injuries of the uterus [ 17 ].…”

Section: Scaffolds In Tissue Engineering—recent Findings and Current ...mentioning

confidence: 99%

Biomimetic Scaffolds—A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering

Górnicki,

Lambrinow,

Golkar-Narenji

et al. 2024

Nanomaterials

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Biomimetic scaffolds imitate native tissue and can take a multidimensional form. They are biocompatible and can influence cellular metabolism, making them attractive bioengineering platforms. The use of biomimetic scaffolds adds complexity to traditional cell cultivation methods. The most commonly used technique involves cultivating cells on a flat surface in a two-dimensional format due to its simplicity. A three-dimensional (3D) format can provide a microenvironment for surrounding cells. There are two main techniques for obtaining 3D structures based on the presence of scaffolding. Scaffold-free techniques consist of spheroid technologies. Meanwhile, scaffold techniques contain organoids and all constructs that use various types of scaffolds, ranging from decellularized extracellular matrix (dECM) through hydrogels that are one of the most extensively studied forms of potential scaffolds for 3D culture up to 4D bioprinted biomaterials. 3D bioprinting is one of the most important techniques used to create biomimetic scaffolds. The versatility of this technique allows the use of many different types of inks, mainly hydrogels, as well as cells and inorganic substances. Increasing amounts of data provide evidence of vast potential of biomimetic scaffolds usage in tissue engineering and personalized medicine, with the main area of potential application being the regeneration of skin and musculoskeletal systems. Recent papers also indicate increasing amounts of in vivo tests of products based on biomimetic scaffolds, which further strengthen the importance of this branch of tissue engineering and emphasize the need for extensive research to provide safe for humansbiomimetic tissues and organs. In this review article, we provide a review of the recent advancements in the field of biomimetic scaffolds preceded by an overview of cell culture technologies that led to the development of biomimetic scaffold techniques as the most complex type of cell culture.

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Production and Characterization of an Integrated Multi-Layer 3D Printed PLGA/GelMA Scaffold Aimed for Bile Duct Restoration and Detection

Cited by 13 publications

References 46 publications

Fabrication and optimization of 3D printed gelatin methacryloyl microneedle arrays based on vat photopolymerization

Fabrication and optimization of 3D printed gelatin methacryloyl microneedle arrays based on vat photopolymerization

Antibacterial CATH‐2 Peptide Coating to Prevent Bone Implant‐Related Infection

Biomimetic Scaffolds—A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering

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