Production and characterization of a thermostable endo-type β-xylanase produced by a newly-isolated Streptomyces thermocarboxydus subspecies MW8 strain from Jeju Island

Chi, Won-Jae; Lim, Ju Hyeon; Park, Da Yeon; Park, Jae-Seon; Hong, Soon-Kwang

doi:10.1016/j.procbio.2013.07.024

Cited by 19 publications

(21 citation statements)

References 25 publications

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“…Cell mass and total xylanase activity derived from cultures of strain WJ-2 in modified medium were almost 9-fold (790 U) higher than those observed in original medium. Strain WJ-2 exhibited maximum total xylanase activities at pH 7.0 and 55℃, which is well within the range of conditions (pH 5.0−7.0 and at 50−65℃) that yield highest xylanase activity for most of the other known xylanases found in Streptomyces species [4,9,15,33,37,38]. The half-life of strain WJ-2 xylanase at 55℃ was 20 min, which is similar to xylanases from Streptomyces sp.…”

Section: Discussionsupporting

confidence: 70%

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Identification and Biochemical Characterization of a New Xylan-degrading Streptomyces atrovirens Subspecies WJ-2 Isolated from Soil of Jeju Island in Korea

Kim¹,

Bae²,

Yeo³

et al. 2016

Microbiology and Biotechnology Letters

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A bacterial strain was isolated from a soil sample collected on Jeju Island, Korea. The strain, designated WJ-2, exhibited a high xylanase activity, whereas cellulase activity was not detected. The 16S rRNA gene sequence of WJ-2 was highly similar to type strains of the genus Streptomyces. A neighbor-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain WJ-2 is phylogenetically related to Streptomyces atrovirens. Furthermore, DNA-DNA hybridization analysis confirmed that strain WJ-2 is a novel subspecies of Streptomyces atrovirens. The genomic DNA G+C content was 73.98 mol% and the major fatty acid present was anteiso-C15:0 (36.19%). The growth and xylanase production of strain WJ-2 were significantly enhanced by using soytone and xylan as nitrogen and carbon sources, respectively. Crude enzyme preparations from the culture broth of strain WJ-2 exhibited maximal total xylanase activities at pH 7.0 and 55℃. Thin-layer chromatography analysis revealed that the crude enzyme degrades beechwood xylan to yield xylobiose and xylotriose as the principal hydrolyzed end products.

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Section: Discussionsupporting

confidence: 70%

“…Hydrolysis of lignocellulosic biomass by microbial enzymes is considered an environmentally friendly alternative to chemical hydrolysis, which generates hazardous byproducts [7]. Thus, several efforts to isolate new microorganisms producing more relevant xylanases have been reported [8,9,19,24].…”

Section: Introductionmentioning

confidence: 99%

Identification and Biochemical Characterization of a New Xylan-degrading Streptomyces atrovirens Subspecies WJ-2 Isolated from Soil of Jeju Island in Korea

Kim¹,

Bae²,

Yeo³

et al. 2016

Microbiology and Biotechnology Letters

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“…The major hydrolysis products were found to be xylobiose, xylotriose, and xylotetrose based on their R f values ( Figure 4). The purified enzyme was an endo-type b-xylanase; hence the R f occurrence of XOS longer than xylobiose as the major product is attributed to endo-type hydrolysis of the polymer as reported earlier (Chi et al, 2013). The FT-IR analysis confirm the purity and identity these sugars, besides indicating the presence of functional groups (Faix, 1991;Goncalves et al, 2012;Ruzene, Silva, Vicente, Goncalves, & Teixeira, 2008).…”

Section: Analysis Of Hydrolytic Productssupporting

confidence: 71%

Production of a thermostable and alkali resistant endoxylanase byBacillus subtilisDFR40 and its application for preparation of prebiotic xylooligosaccharides

2018

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Xylanases produced by microorganisms hydrolyze xylan, into xylose or xylooligosaccharides (XOS). Xylan is an abundant noncellulosic polysaccharide found in the plant biomass. The enzyme is of great practical importance for the conversion of xylan containing agricultural residues into XOS for use as prebiotics. A Bacillus subtilis strain DFR40 a generally recognized as safe (GRAS) organism was found to produce extracellular xylanase in a growth medium containing wheat bran, mineral salts, peptone, and yeast extract at pH 7.0. The optimum activity of the enzyme was around 100°C and pH 9.0 with stability over a broad range of pH (7.0–12.0) displaying enzyme stability of more than 70% even after pre‐incubation at 55–85°C for 30 min. The enzyme hydrolyzed wheat bran into short chain XOS as revealed by TLC, HPLC, FT‐IR, and NMR analysis. Practical applications The thermostable and alkali resistant characteristics of extracted xylanase along with ability to hydrolyze wheat bran for the production of xylooligosaccharides (XOS), for use as prebiotic ingredients in food, are relevant in the present health conscious worldwide scenario. XOS are emerging class of prebiotics with thermal resistance up to 100°C, and are suitable for incorporating into thermally processed food products with extended shelf life. The XOS preparation was also found suitable suggesting the prebiotic nature of XOS on known probiotic bacterial strains. The study also indicates a valuable application for wheat bran, a cheap agro residue for further cost effective scale up of XOS production. The xylanase produced is of significance as the producing bacterium is a GRAS organism for further scale‐up of the processes of enzyme and XOS production.

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“…Xylanases are used in industrial applications such as in pulp prebleaching processes, as food and feed additives, in bakery processing, and in xylitol production, among others [1]. However, a great attention has been devoted to its utilization in hemicellulosic biomass degradation, aiming at production of biofuels.…”

Section: Introductionmentioning

confidence: 99%

“…Synergic effects of alfa-arabinofuranosidase (EC 3.2.1.55), beta-D-glucuronidase (EC 3.2.1.1), and acetyl xylan esterase (EC 3.2.11.6) are required to release the side chains of xylan. However, two enzymes, namely beta-1,4-endoxylanases (EC 3.2.1.8) and beta-xylosidase (EC 3.2.1.37), are sufficient to degrade the xylan back-bone [1,7].…”

Section: Introductionmentioning

confidence: 99%

Trichoderma atroviride 102C1 Mutant: A High Endoxylanase Producer for Assisting Lignocellulosic Material Degradation

Oliveira¹

2014

Microb Biochem Technol

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Endoxylanases have played an important role in many industrial processes as bleachers to kraft pulp, animal feeds and baked goods. Also, nowadays, a special attention has been devoted to the role of these enzymes in saccharification of lignocellulose biomass for biofuels production. Trichoderma species are among fungi those that have been most extensively studied, due to their efficient production of these enzymes. Among the different strategies for improving the production and biochemical aspects of enzymes of commercial interest, mutations induced using chemical agents and/or physical devices can be cited. In the present strain T. atroviride 102C1 was obtained by using UV light and nitrosoguanidine as mutagenic agents. A factorial design (central composite rotational design, CCRD) was performed to estimate the optimal levels of C (sugarcane bagasse) and N (corn steep liquor) sources for best xylanase production. After the CCRD, the 102C1 mutant strain showed increased activity of 340% for xylanase production when compared to the wild type. The enzyme was partially characterized according to its pH and temperature profile, also using CCRD. The characterization of 102C1 mutant strain as a high endoxylanase producer allows its use in biotechnological applications, particularly in the hydrolysis of lignocellulosic biomass for biorefinary proposes.

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Production and characterization of a thermostable endo-type β-xylanase produced by a newly-isolated Streptomyces thermocarboxydus subspecies MW8 strain from Jeju Island

Cited by 19 publications

References 25 publications

Identification and Biochemical Characterization of a New Xylan-degrading Streptomyces atrovirens Subspecies WJ-2 Isolated from Soil of Jeju Island in Korea

Identification and Biochemical Characterization of a New Xylan-degrading Streptomyces atrovirens Subspecies WJ-2 Isolated from Soil of Jeju Island in Korea

Production of a thermostable and alkali resistant endoxylanase byBacillus subtilisDFR40 and its application for preparation of prebiotic xylooligosaccharides

Trichoderma atroviride 102C1 Mutant: A High Endoxylanase Producer for Assisting Lignocellulosic Material Degradation

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