Trichoderma atroviride 102C1 Mutant: A High Endoxylanase Producer for Assisting Lignocellulosic Material Degradation

Abstract

Endoxylanases have played an important role in many industrial processes as bleachers to kraft pulp, animal feeds and baked goods. Also, nowadays, a special attention has been devoted to the role of these enzymes in saccharification of lignocellulose biomass for biofuels production. Trichoderma species are among fungi those that have been most extensively studied, due to their efficient production of these enzymes. Among the different strategies for improving the production and biochemical aspects of enzymes of commercial interest, mutations induced using chemical agents and/or physical devices can be cited. In the present strain T. atroviride 102C1 was obtained by using UV light and nitrosoguanidine as mutagenic agents. A factorial design (central composite rotational design, CCRD) was performed to estimate the optimal levels of C (sugarcane bagasse) and N (corn steep liquor) sources for best xylanase production. After the CCRD, the 102C1 mutant strain showed increased activity of 340% for xylanase production when compared to the wild type. The enzyme was partially characterized according to its pH and temperature profile, also using CCRD. The characterization of 102C1 mutant strain as a high endoxylanase producer allows its use in biotechnological applications, particularly in the hydrolysis of lignocellulosic biomass for biorefinary proposes.

Production of thermophilic and acidophilic endoglucanases by mutant Trichoderma atroviride 102C1 using agro-industrial by-products

“…Therefore, the development of new organisms with lignocellulolytic capacities is crucial to make this process economically viable. Recently, Oliveira et al (2014) has characterized this same 102C1 mutant strain as an excellent xylanase producer in comparison to wild strain and others Trichoderma species. The authors are convinced that our fermentation results prove that our mutant might be suitable strains for practical applications, and the selection of a new mutanttype T. atroviride 102C1 as a good cellulase producer, allows its use in biotechnological applications, particularly in the hydrolysis of agro-industrial by-products, such as sugarcane bagasse and straw, for bioethanol production.…”

A Thermotolerant Xylan-Degrading Enzyme Is Produced by Streptomyces malaysiensis AMT-3 Using by-Products From the Food Industry

“…Since endoxylanases randomly cleave the xylan backbone, the reaction products are diverse, which may include xylose, xylobiose, xylooligomers, heterodisaccharides of xylose and glucose and their positional isomers [5]. Endoxylanases are produced by many mesophilic microorganisms, and have already been found in bacteria, such as Bacillus and Clostridium species [3,4,9], in several species of Streptomyces [10][11][12], and in fungi such as Thermomyces, Aspergillus and Trichoderma [6,13,14].…”

Trichoderma atroviride 102C1: A promising mutant strain for the production of a -glucosidase, -xylosidase and -L-arabinofuranosidase activities using agroindustrial by-products

“…Mutant strain T. atroviride 102C1 was obtained from the wild strain T. atroviride 676 (Grigorevski-Lima et al, 2013), after successive exposures to U.V. radiation and nitrosoguanidine, according to Oliveira et al (2014). Spore suspensions were prepared according to Hopwood et al (Hopwood et al, 1985), after cultivation at 28°C for 7 days in potato dextrose agar medium (Hanada et al, 2002).…”

“…Trichoderma atroviride 676, isolated from Amazon forest soil, was capable to produce cellulases and endoxylanases when agro-industrial by-products were used as substrates (Grigorevski-Lima et al, 2013). After mutation procedures in this strain, a new mutant, T. atroviride 102C1, producer of high titers of endoglucanase, exoglucanase and endoxylanase was obtained (Oliveira et al, 2014). The objective of the present study was to investigate the production of some hollocellulolytic accessory enzymes of T. atroviride 102C1, including β-glucosidase, β-xylosidase and α-Larabinofuranosidase, using submerged fermentation and, as main substrates, sugarcane bagasse in natura and corn steep liquor.…”