Production and characterization of a broad-specificity polyclonal antibody for O,O-diethyl organophosphorus pesticides and a quantitative structure–activity relationship study of antibody recognition

Monoclonal antibody-based homogeneous immunoassay for three banned agonists and molecular modeling insight

“…The higher CR for BRO and CLE might be explained by the existing -NH 2 group and the halogen atoms. Both the -NH 2 group and the halogen atoms were electronegative and had strong electron-donating ability, which may improve the antibody recognition (Xu, Xie, et al, 2009). Compared with the chlorine atom, the bromine atom was more electronegative, which might be the reason why the CR for BRO was higher than that for CLE.…”

Preparation of dichlorvos–protein complete antigen by Mannich-type reaction

“…Nowadays, some immunological methods, such as enzyme-linked immunosorbent assay (ELISA), are more and more popular, and have been used to detect parathion-methyl [17], fenthion [29], fenitrothion [26], diazinon [4], isofenphos [25] and soman [18]. Broad-specificity polyclonal antibodies were obtained to detect O,O-dimethyl organophosphorus [30] and O,O-diethyl organophosphorus [32]. However, to our knowledge there is no report about a DDVP immunoassay.…”

Simultaneous determination of phoxim and its photo-transformation metabolite residues in eggs using liquid chromatography coupled with electrospray ionization tandem mass spectrometry

“…Residue methods involving high-performance liquid chromatography (UV and/or DAD) detectors have been previously reported for the detection of phoxim in Crucian carp [11], in eggs [3,4], in wheat [12], and in water [13,14]. Additionally, methods using polyclonal antibody [15] or chemometrics [16] have also been previously described. Combined liquid chromatography-mass spectrometry using electrospray or atmospheric-pressure chemical ionization has become a critically important tool in the quantitative analysis of pesticide residues in various matrices in relation to environmental analysis, food safety, and biological exposure monitoring.…”