Production and characteristics of some new β-agarases from a marine bacterium, Vibrio sp. strain JT0107

Sugano, Yasushi; Nagae, Hideki; Inagaki, Koji; Yamamoto, Takeshi; Terada, Ichiro; Yamazaki, Yoshinari

doi:10.1016/0922-338x(95)94746-e

Cited by 39 publications

(44 citation statements)

References 19 publications

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“…JT0107 with a high molecular mass of 105 kDa shows maximal activity at pH 8 and hydrolyzes not only agarose but also NA4 to yield NA2. 33) rAgaA (AgaA) is much more beneficial than the reported enzymes for use in industrial applications. The high activity and thermostability at temperatures higher than the gelling temperature of agar (around 40 C) represent advantages for industrial oligosaccharide production from agar or marine algae.…”

Section: Discussionmentioning

confidence: 99%

Enzymatic Properties and Nucleotide and Amino Acid Sequences of a Thermostable β-Agarase from the Novel Marine Isolate, JAMB-A94

Ohta¹,

Nogi²,

Miyazaki³

et al. 2004

Bioscience, Biotechnology, and Biochemistry

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Section: Discussionmentioning

confidence: 99%

Enzymatic Properties and Nucleotide and Amino Acid Sequences of a Thermostable β-Agarase from the Novel Marine Isolate, JAMB-A94

Ohta¹,

Nogi²,

Miyazaki³

et al. 2004

Bioscience, Biotechnology, and Biochemistry

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“…citric acid) or ␣-agarase can provide agaro-oligosaccharides to cleave the ␣-1,3 bond (29)(30)(31). In the present study, we noted the fact that GSH levels of plasma and cecal contents in the group of Gelidium sp, TDF, SDF, DFFC and deionized DFFC had remained rather higher than those of the Control group in spite of the lack of change in the liver GSH (Table 6).…”

Section: Discussionmentioning

confidence: 48%

Effects of Various Kinds of Edible Seaweeds in Diets on the Development of D-Galactosamine-Induced Hepatopathy in Rats

Kawano

Egashira

Sanada

2007

J Nutr Sci Vitaminol

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SummaryIn the present study we investigated the effects of 11 kinds of edible seaweeds (6 brown and 5 red algae) which contain characteristic seaweed dietary fibers on the induction of D -GalN ( D -galactosamine)-hepatopathy in rats (Exps. 1 and 2). Then, the efficacy of various components prepared from Gelidium sp., which was found to alleviate the hepatopathy in Exps. 1 and 2, was examined (Exp. 3). The rats were fed the diets containing various kinds of seaweeds (Exps. 1 and 2), or several components of Gelidium sp. such as total dietary fiber (TDF), soluble dietary fiber (SDF), insoluble dietary fiber (IDF) and dietary fiber-free components (DFFC) (Exp. 3), for 8 d. The rats in all experiments were injected with D -GalN (800 mg/kg body weight) intraperitoneally at the 7th day to induce liver injury and were sacrificed 24 h after the injection of D -GalN. The serum transaminase activities (ALT and AST) and lactate dehydrogenase (LDH) were determined to evaluate the levels of hepatopathy. In Exp. 3, the total GSH concentration in the liver, plasma and cecal contents and organic acid concentration in cecal contents were also evaluated. In Exps. 1 and 2, repressive effects against D -GalN-hepatopathy were shown by four seaweeds Laminaria sp., Gelidium sp., Sargassum fulvellum and Eisenia bicyclis . In Exp. 3, it was found that protective activity in Gelidium sp. against D -GalN-hepatopathy existed not only in the SDF but also in the DFFC fraction. The results in Exp. 3 also indicated that the total GSH but not organic acid concentration in the cecal contents were significantly correlated with serum AST activity, suggesting that the protective effect of Gelidium sp. on D -GalN-hepatopathy in rats is related to GSH metabolism in the intestine. Key Words seaweed, D -galactosamine, Gelidium , agar, glutathione This research was conducted for investigation of the influence of seaweeds on the development of D -galactosamine-induced hepatopathy ( D -GalN-hepatopathy) in rats. Because D -GalN-hepatopathy resembles human acute viral hepatitis histologically, D -galactosamine ( DGalN) intoxication is used as an animal model of fulminant hepatic failure ( 1 , 2 ). D -GalN is converted to UDP-D -GalN by the enzymes in the galactose metabolic pathway in the liver ( 3 ). The exhaustion of UTP caused by the UDP-D -GalN formation process is accompanied by a decrease on hepatocellular UDP, UTP, and UDP-glucose levels ( 4 -6 ). Then, D -GalN prevents the synthesis of RNA and protein in the liver, which leads to a defect in some important cell membrane proteins. Besides the above-mentioned causes, the D -GalN-induced-hepatopathy is thought to be also induced by the increase of mesentery permeability of endotoxin which is a kind of natural hepatotoxin in the intestine ( 7 , 8 ). According to previous studies, bacterial translocation in D -GalNhepatopathy was reduced by intestinal excision or use of an antibiotic which reduces intestinal bacteria in the animals before D -GalN-treatment ( 9 , 10 ). In other reports, medication with g...

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“…Alginase activity could also be detected in the cultures with all other tested nitrogen sources, but was lower than that in the ammonium nitrate containing media. Previous findings suggested that peptone was an important or essential component of growth medium useful for bacterial growth and carbohydrate lyase production [Sugano et al, 1995 andAlexeeva et al, 2002]. Peptone was found to be an unfavorable nitrogen source for International Letters of Natural Sciences Vol.…”

Section: Discussionmentioning

confidence: 99%

Media Optimization for Depolymerization of Alginate by <i>Pseudomonas aeruginosa</i> AG LSL-11

Koti

Shinde

Lalitha

2014

ILNS

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An agar degrading bacterium Pseudomonas aeruginosa AG LSL-11 was acclimatized to alginate for the production of alginase. Production parameters such as pH, temperature, influence of simple carbohydrates and nitrogen sources, and effect of NaCl on growth and alginase production were carried out. Maximum growth was observed at pH 9.0 and 35 °C, while alginase was produced optimally at pH 9.0 and 30 °C. The alginase produced by Pseudomonas aeruginosa AG LSL-11 was inducible by alginate, and repressed by other simple sugar when supplemented along with alginate in the medium. The bacterium did not require NaCl for growth and production of alginase. The activity staining of partially purified culture supernatant after native PAGE revealed the presence of a single alginase.

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Production and characteristics of some new β-agarases from a marine bacterium, Vibrio sp. strain JT0107

Cited by 39 publications

References 19 publications

Enzymatic Properties and Nucleotide and Amino Acid Sequences of a Thermostable β-Agarase from the Novel Marine Isolate, JAMB-A94

Enzymatic Properties and Nucleotide and Amino Acid Sequences of a Thermostable β-Agarase from the Novel Marine Isolate, JAMB-A94

Effects of Various Kinds of Edible Seaweeds in Diets on the Development of D-Galactosamine-Induced Hepatopathy in Rats

Media Optimization for Depolymerization of Alginate by <i>Pseudomonas aeruginosa</i> AG LSL-11

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