Media Optimization for Depolymerization of Alginate by &lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt; AG LSL-11

Koti, Basawaraj A.; Shinde, Manohar; Lalitha, Junna

doi:10.18052/www.scipress.com/ilns.19.30

Cited by 3 publications

(3 citation statements)

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“…Therefore, to sustain their exhausted growth, surviving cells might have degraded the alginate into oligosaccharides of respective constituents as a consumable carbon source using extracellular alginate lyase (Koti et al, 2014 ).…”

Section: Resultsmentioning

confidence: 99%

“…These results also suggested that nutrients, including the carbon source, were available for sustainable growth and alginate production up to 150 h after which prolonged incubation led to a reduction in yields due to nutrient scarcity (Manresa et al, 1987). Therefore, to sustain their exhausted growth, surviving cells might have degraded the alginate into oligo-saccharides of respective constituents as a consumable carbon source using extracellular alginate lyase (Koti et al, 2014).…”

Section: Quantification Of Cell Growth and Alginate Biosynthesismentioning

confidence: 93%

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Promising substitute of inconsistent algal alginates: exploring the biocompatible properties of di-O-acetylated, poly-L-guluronate-deficient alginate from soil bacterium Pseudomonas aeruginosa CMG1418

Muhammadi¹,

Shafiq²,

Rizvi³

2023

bta

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The structural inconsistencies in commercial algal alginates have limited their reliability and quality for various applications. Therefore, the biosynthesis of structurally consistent alginates is crucial to replace the algal alginates. Thus, this study aimed to investigate the structural and alginate's structural and functional properties of Pseudomonas aeruginosa CMG1418 as a substitute. To achieve this, the CMG1418 alginates were physiochemically characterized using various techniques such as transmission electron microscopy, Fourier-transform infrared 1 H-NMR, 13 C-NMR, and gel permeation chromatography. The synthesized CMG1418 alginate was then subjected to standard tests to evaluate its biocompatibility, emulsification, hydrophilic, flocculation, gelling, and rheological properties. The analytical studies revealed that CMG1418 alginate is an extracellular and polydisperse polymer with a molecular weight range of 20 000-250 000 Da. It comprises 76% poly-(1-4)-β-D-mannuronic acid (M-blocks), no poly-α-L-guluronate (G-blocks), 12% alternating sequences of β-D-mannuronic acid and α-L-guluronic acid (poly-MG/GM-blocks), 12% MGM-blocks, 172 degrees of polymerization, and di-O-acetylation of M-residues. Interestingly, CMG1418 alginate did not show any cytotoxic or antimetabolic activity. Moreover, compared to algal alginates, CMG1418 alginate exhibited higher and more stable flocculation efficiencies (70-90%) and viscosities (4500-4760 cP) over a wide range of pH and temperatures. Additionally, it displayed soft to flexible gelling abilities and higher water-holding capacities (375%). It also showed thermodynamically more stable emulsifying activities (99-100%) that surpassed the algal alginates and commercial emulsifying agents. However, only divalent and multivalent cations could slightly increase viscosity, gelling, and flocculation. In conclusion, this study explored a structurally di-O-acetylated and poly-G-blocks-deficient, biocompatible alginate, and its pH and thermostable functional properties. This research suggests that CMG1418 alginate is a superior and more reliable substitute for algal alginates in various applications, such as viscosifying, soft gelling, flocculating, emulsifying, and waterholding.

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Section: Resultsmentioning

confidence: 99%

Section: Quantification Of Cell Growth and Alginate Biosynthesismentioning

confidence: 93%

Promising substitute of inconsistent algal alginates: exploring the biocompatible properties of di-O-acetylated, poly-L-guluronate-deficient alginate from soil bacterium Pseudomonas aeruginosa CMG1418

Muhammadi¹,

Shafiq²,

Rizvi³

2023

bta

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“…Pseudomonas aeruginosa is causing septicemia, cystic fibrosis (CF), infections of the respiratory tract, burn wounds, urinary tract, and blood. The opportunistic pathogen P.aeruginosa secretes of alginate as a capsule-like polysaccharide, that has an im-portant role in evasion of host defenses, especially during the chronic pulmonary disease of patients with cystic fibrosis (20). Researchers have shown that alginate lyase therapy (inhibition) can decrease viscosity in cultures of clinical isolates and in CF sputum; dissociates biofilms from abiotic surfaces and enhances the phagocytosis and manslaughter of P.aeruginosa by human immune cells, and also improves the efficacy of various antibiotics.…”

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confidence: 99%

Production, Purification and Inhibition of Alginate Ly-Ase From Local Isolate of Pseudomonas Aeruginosa Na11

Younis¹

2020

Iraqi J. Agric. Sci.

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The point of this study was for determine of the optimum conditions and purification of alginate lyase from local isolate of Pseudomonas aeruginosa NA11, and inhibition of enzyme by various plant extracts. Forty local isolate s of pathogenic Pseudomonas aeruginosa were screened for their ability to produce alginate lyase. Local isolate of P.aeruginosa NA11 showed the maximum efficiency for produce of alginate lyase with high specific activity (14.4 U/mg). Several factors that influence on alginate lyase production from local isolate of P.aeruginosa NA11 were studied, these factors included the type of media, carbon source, nitrogen source, the incubation temperature, pH, and the incubation period. The highest yield of alginate lyase was obtained with the A medium supplemented with 0.5 % of glucose and sodium nitrate at pH 7.5 after 24 hr. incubation at 37 °C. Two chromatographic techniques were used for purification of alginate lyase after precipitation by ammonium sulphate with saturated ratio (0-70 %), including, ion exchange chromatography by DEAE-cellulose and gel filtration by Sephadex G-100. The two steps gave the specific activity of 155.8 U/mg protein, the purification fold was 4.15 and enzymatic yield was 64 %. The molecular weight of partial purified alginate lyase was 57 KDa. The results of antioxidant activity tests for different plants extracts utilizing DPPH radical scavenging activity were showed that the saad extract has high antioxidant activity (71 %) more than other plants extracts. While the results for inhibition experiment of alginate lyase were demonstrated that saad extract was the best inhibitor with inhibition ratio of 86 %.

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Alginate Lyase Produced by Filamentous Fungus Through Solid State Fermentation Using Sargassum from the Brazilian Coast

Silva

Santos

et al. 2022

Waste Biomass Valor

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Media Optimization for Depolymerization of Alginate by <i>Pseudomonas aeruginosa</i> AG LSL-11

Cited by 3 publications

References 4 publications

Promising substitute of inconsistent algal alginates: exploring the biocompatible properties of di-O-acetylated, poly-L-guluronate-deficient alginate from soil bacterium Pseudomonas aeruginosa CMG1418

Promising substitute of inconsistent algal alginates: exploring the biocompatible properties of di-O-acetylated, poly-L-guluronate-deficient alginate from soil bacterium Pseudomonas aeruginosa CMG1418

Production, Purification and Inhibition of Alginate Ly-Ase From Local Isolate of Pseudomonas Aeruginosa Na11

Alginate Lyase Produced by Filamentous Fungus Through Solid State Fermentation Using Sargassum from the Brazilian Coast

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