Production and Application of Doubled Haploid in Brassica Improvement

Watts, Anshul; Sankaranarayanan, Subramanian; Raipuria, Ritesh Kumar; Watts, Archana

doi:10.1007/978-3-030-34694-2_4

Cited by 7 publications

(13 citation statements)

References 81 publications

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“…Notably, in B. napus, it has been estimated that microspore culture is tenfold efficient than the anther culture (Siebel and Pauls 1989). Especially, double haploid production through microspore culture has effectively used in B. rapa, B. oleracea, B. napus, B. carinata, and B. juncea and numerous varieties had developed in B. napus and B. oleracea (Watts et al 2020) and numerically it is above 50 .…”

Section: Brassica Perspectivesmentioning

confidence: 99%

“…Various factors have a direct effect on haploid and double haploid production of Brassica and the principal factors had stated briefly by Watts et al (2020). Especially for white cabbage, the responsible factor had been optimized for the ease of microspore embryogenesis and double haploid production (Bhatia et al 2021).…”

Section: Brassica Perspectivesmentioning

confidence: 99%

“…Especially, the responsible loci for embryogenesis have been identified in B. napus and B. campestris (Cloutier et al 1995;Ajisaka et al 1999). Then, the growth environment of the donor plant has a great role in anther or microspores derive embryogenesis (Watts et al 2020). In most cases, plants are grown at low temperatures responded significantly in Brassica microscope embryogenesis (Watts et al 2020).…”

Section: Brassica Perspectivesmentioning

confidence: 99%

See 2 more Smart Citations

Anther Culture in Crop Plants: Progress and Perspectives

Islam

Arif

Hasan

et al. 2023

Plant Breed. Biotech.

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A resurrection has started in haploid and double haploid research in the twenty-first century. The haploid and double haploid could be achieved through in vivo and in vitro anther and microspore culture techniques. Fixing the homozygosity is the most striking benefit of androgenesis. Various factors like genotypic dependency, growth condition, developmental stage of the microspore, pre-treatment, culture media, regeneration media, growth hormones, and various chemicals have a direct effect. Wheat, rice, Brassica, and tobacco are the notable crops where anther and microspore culture has been utilized. These haploidy and double haploidy through anther culture served many purposes of basic and applied research. Especially, double haploid cultivars have been cultivating around the globe. In addition, for chromosome mapping, QTL mapping, marker-assisted selection, marker-assisted backcrossing, mutation breeding, genome-wide association study, genomic engineering, and genome editing, androgenesis based haploid and double haploid plants have been exploited due to the effectiveness. Recently, researchers are trying to explain albinism that happens during anther culture from an epigenetic perspective. Further prospects of haploid and doubled haploid research through anther culture have been described in this review.

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Section: Brassica Perspectivesmentioning

confidence: 99%

Section: Brassica Perspectivesmentioning

confidence: 99%

Section: Brassica Perspectivesmentioning

confidence: 99%

See 1 more Smart Citation

Anther Culture in Crop Plants: Progress and Perspectives

Islam

Arif

Hasan

et al. 2023

Plant Breed. Biotech.

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“…This system is very useful for generating haploid and doubled haploid plants for breeding [ 34 ], but from a practical point of view microspores have serious limitations for studying cell reprogramming: (i) a long time is needed to obtain sufficient starting material. For example, even the rapid life cycle of Arabidopsis takes at least 40 days to produce microspores, and in other species, this could be much longer; (ii) very precisely controlled greenhouse conditions are required at different stages of plant development [ 37 , 38 ]; (iii) after meiosis, microspores undergo differentiation very rapidly, which leads to a low frequency of cells undergoing reprogramming; and (iv) only a limited number of genotypes have a relatively high frequency of embryogenesis [ 38 , 39 ].…”

Section: Experimental Systems For Exploiting Totipotencymentioning

confidence: 99%

From Single Cell to Plants: Mesophyll Protoplasts as a Versatile System for Investigating Plant Cell Reprogramming

Pasternak

Lystvan

Betekhtin

et al. 2020

IJMS

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Plants are sessile organisms that have a remarkable developmental plasticity, which ensures their optimal adaptation to environmental stresses. Plant cell totipotency is an extreme example of such plasticity, whereby somatic cells have the potential to form plants via direct shoot organogenesis or somatic embryogenesis in response to various exogenous and/or endogenous signals. Protoplasts provide one of the most suitable systems for investigating molecular mechanisms of totipotency, because they are effectively single cell populations. In this review, we consider the current state of knowledge of the mechanisms that induce cell proliferation from individual, differentiated somatic plant cells. We highlight initial explant metabolic status, ploidy level and isolation procedure as determinants of successful cell reprogramming. We also discuss the importance of auxin signalling and its interaction with stress-regulated pathways in governing cell cycle induction and further stages of plant cell totipotency.

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“…breeding programs, in vitro plant tissue cultures has been involved in a wide range of applications. Among these, haploid production through anther culture proved to be an important approach of tissue culture, during the last decades (Shariatpanahi and Ahmadi, 2016;Anshul et al, 2020). If traditionally, breeders can obtain homozygosity by selfpollination, in 8-10 years, with anther culture, homozygous plant can be produced within one year.…”

Section: Introductionmentioning

confidence: 99%

EFFECT OF THE MAIN CYTOKININS ON ANDROGENESIS OF WHITE CABBAGE (BRASSICA OLERACEA L. var. CAPITATA) ANTHERS CULTIVATED “IN VITRO”

Cristea

Iosob

Brezeanu

et al. 2020

RJH

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The aim of the present research work was the screening of the effect of the main cytokinin (BAP, kinetin or zeatin) in different concentrations and combinations with the auxin NAA on androgenesis of white cabbage anthers cultivated in vitro. The results obtained are regarded as an intermediary stage for the development of a reproducible protocol for in vitro regeneration of plant from anther culture. Thus, for the determination of the influence of plant growth regulators formula over the callus induction and plant regeneration from anthers cultivated in vitro in the present study the authors undergo a screening of the three most frequently utilized cytokinins (BAP, kinetin and zeatin) in different concentration and combination with the auxin NAA. The results obtained, indicated that the best morphogenetic reaction is obtained on variant with BAP as the main growth regulator.

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Production and Application of Doubled Haploid in Brassica Improvement

Cited by 7 publications

References 81 publications

Anther Culture in Crop Plants: Progress and Perspectives

Anther Culture in Crop Plants: Progress and Perspectives

From Single Cell to Plants: Mesophyll Protoplasts as a Versatile System for Investigating Plant Cell Reprogramming

EFFECT OF THE MAIN CYTOKININS ON ANDROGENESIS OF WHITE CABBAGE (BRASSICA OLERACEA L. var. CAPITATA) ANTHERS CULTIVATED “IN VITRO”

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