Product Removal Strategy and Fouling Mechanism for Cellulose Hydrolysis in Enzymatic Membrane Reactor

Lim, Shin Yuan; Ghazali, Nazlee Faisal

doi:10.1007/s12649-020-01020-6

Cited by 5 publications

(6 citation statements)

References 47 publications

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“…Although UF based MBR was effective to retain the enzyme and limit enzyme product inhibition, the system was prone to membrane fouling. As a strategy to limit membrane fouling, Lim and Ghazali (2020) used an intermittent product removal during the continuous hydrolysis of microcystalline cellulose. The removal of the product from the biore actor using UF membrane filtration was done under two different stra tegies.…”

Section: Disco11timwus Mbr A11d Product I11hibiti011mentioning

confidence: 99%

“…However, a side-stream configuration is a limiting factor to successful large scale applications, since pumping a slurry imparts a significant operating cost (Roche et al, 2009;Stickel et al, 2009). Moreover, low MWCO membranes require high transmembrane pressure and leads to significant membrane fouling (Lim & Ghazali, 2020;Lozano et al, 2014;Mahboubi et al, 2017a). While a continuously fed MBR could face se vere membrane fouling, owing to the enzyme retention and sinmlta neous product removal, a continuously/intermittently fed system can have better productivity.…”

Section: Continuously Fed Mbr Lim Itation To Low Mwco Membrane and Op...mentioning

confidence: 99%

“…Yet, the obtained product concentration in many of the studies is considerably low (0.2-20 g/L,) (Gebreyohannes et al, 2018;Lim & Ghazali, 2020;Lozano et al, 2014;Zhang et al, 2011). Since the desired concentration for subsequent fermentation to ethanol, falls between 150 and 250 g/L glucose (Malmali et al, 2015), a significant energy is consumed in pre-concentration.…”

Section: Continuously Fed Mbr Lim Itation To Low Mwco Membrane and Op...mentioning

confidence: 99%

See 2 more Smart Citations

Enzyme catalysis coupled with artificial membranes towards process intensification in biorefinery- a review

Mazzei

Gebreyohannes

Papaioannou

et al. 2021

Bioresource Technology

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Section: Disco11timwus Mbr A11d Product I11hibiti011mentioning

confidence: 99%

Section: Continuously Fed Mbr Lim Itation To Low Mwco Membrane and Op...mentioning

confidence: 99%

Section: Continuously Fed Mbr Lim Itation To Low Mwco Membrane and Op...mentioning

confidence: 99%

See 1 more Smart Citation

Enzyme catalysis coupled with artificial membranes towards process intensification in biorefinery- a review

Mazzei

Gebreyohannes

Papaioannou

et al. 2021

Bioresource Technology

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“…It is related to concentration polarization, fouling of the membrane, and cake layer formation, which lead to low permeate yields. [14,26] The membrane fouling mechanism based on filter cake formation is the most serious concern, especially at high insoluble solids in the feed. These phenomena limit the application of ultrafiltration of lignocellulosic hydrolysates in industrial conditions.…”

Section: Introductionmentioning

confidence: 99%

Hydrodynamic studies of innovative membrane reactor for enzymatic hydrolysis of lignocellulosic waste

Dąbkowska‐Susfał,

Lipińska,

Sobieszuk

et al. 2024

Biotechnology Journal

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This paper presents the study concerning the impact of the basic operational parameters on the performance of an innovative microfiltration membrane reactor applied for enzymatic hydrolysis of lignocellulosic biomass. The concept and basic hydrodynamics of the reactor with tubular ceramic membranes and a propeller agitator were shown. Besides, the efficiency of enzymatic hydrolysis of corn straw was studied to check reactor functionality. It has been proven that the proposed reactor construction can improve the microfiltration of lignocellulosic suspension by reducing the cake layer on the membrane surface. Increasing the rotational speed of the propeller agitator also improved the filtration efficiency. The permeate flux during the microfiltration experiments was lower for smaller lignocellulose biomass fraction (D < 425 μm) when compared to the less fragmented corn straw (425 < D < 900 μm). For larger solid fractions, a stirring speed increase enhanced the separation efficiency regardless of the differences in biomass concentration. In contrast, this trend for the finer biomass fraction was only noticeable for the highest used biomass concentration (C = 2.0%). Considering the enzymatic hydrolysis of corn straw, membrane separation of reaction products positively influenced the process yield, and the results depended on the applied operational parameters.

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“…Besides eliminating the internal pumping, the in situ continuous product separation had another positive effect on pushing the reaction forward. However, the system suffered from insufficient mixing due to substrate accumulation at the bottom, near the membrane surface, which also caused severe membrane fouling [ 16 , 17 , 18 ]. In our previous work [ 19 ], an MBR with an inverted dead-end concept was tested using standard cellulose as a substrate.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Enzymatic Cellulose Hydrolysis and Product Separation in a Radial-Flow Membrane Bioreactor

et al. 2022

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Hydrolysis is the heart of the lignocellulose-to-bioethanol conversion process. Using enzymes to catalyze the hydrolysis represents a more environmentally friendly pathway compared to other techniques. However, for the process to be economically feasible, solving the product inhibition problem and enhancing enzyme reusability are essential. Prior research demonstrated that a flat-sheet membrane bioreactor (MBR), using an inverted dead-end filtration system, could achieve 86.7% glucose yield from purified cellulose in 6 h. In this study, the effectiveness of flat-sheet versus radial-flow MBR designs was assessed using real, complex lignocellulose biomass, namely date seeds (DSs). The tubular radial-flow MBR used here had more than a 10-fold higher membrane surface area than the flat-sheet MBR design. With simultaneous product separation using the flat-sheet inverted dead-end filtration MBR, a glucose yield of 10.8% from pretreated DSs was achieved within 8 h of reaction, which was three times higher than the yield without product separation, which was only 3.5% within the same time and under the same conditions. The superiority of the tubular radial-flow MBR to hydrolyze pretreated DSs was confirmed with a glucose yield of 60% within 8 h. The promising results obtained by the novel tubular MBR could pave the way for an economic lignocellulose-to-bioethanol process.

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Product Removal Strategy and Fouling Mechanism for Cellulose Hydrolysis in Enzymatic Membrane Reactor

Cited by 5 publications

References 47 publications

Enzyme catalysis coupled with artificial membranes towards process intensification in biorefinery- a review

Enzyme catalysis coupled with artificial membranes towards process intensification in biorefinery- a review

Hydrodynamic studies of innovative membrane reactor for enzymatic hydrolysis of lignocellulosic waste

Simultaneous Enzymatic Cellulose Hydrolysis and Product Separation in a Radial-Flow Membrane Bioreactor

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