Prodelphinidin B-4 3′-O-gallate, a tea polyphenol, is involved in the inhibition of COX-2 and iNOS via the downregulation of TAK1-NF-κB pathway

Hou, De‐Xing; Luo, Dong; Tanigawa, Shunsuke; Hashimoto, Fumio; Uto, Takuhiro; Masuzaki, Satoko; Fujii, Makoto; Sakata, Yusuke

doi:10.1016/j.bcp.2007.06.006

Cited by 64 publications

(39 citation statements)

References 45 publications

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“…Prodelphinidin B-2 3,3′ di-O-gallate, a green tea proanthocyanidin, caused an inhibition of COX-2 via the suppression on the activations of MAPK, including JNK, ERK, and p38 MAPK, and the subsequent blockage on MAPK-mediated activation of NF-κB, and AP-1 and CCAAT/enhancer-binding protein (C/ EBP) δ [39]. Prodelphinidin B-4 3′-O-gallate, other tea polyphenol consisting of gallocatechin and epigallocatechin-3-gallate, was identified to be involved in the inhibition of COX-2 and iNOS via the down-regulation of TGF-β-activated kinase (TAK1)-NF-κB pathway in LPS-activated RAW264.7 macrophage cells [40]. Together with the previous findings, our results propose that the galloyl moiety might have anti-inflammatory activity.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory Activity of n-Propyl Gallate Through Down-regulation of NF-κB and JNK Pathways

et al. 2010

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The present study aimed to assess anti-inflammatory activity and underlying mechanism of n-propyl gallate, the n-propyl ester of gallic acid. n-Propyl gallate was shown to contain anti-inflammatory activity using two experimental animal models, acetic acid-induced permeability model in mice, and air pouch model in rats. It suppressed production of nitric oxide and induction of inducible nitric oxide synthase and cyclooxygenase-2 in the lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. It was able to diminish reactive oxygen species level elevated in the LPS-stimulated RAW264.7 macrophage cells. It also suppressed gelatinolytic activity of matrix metalloproteinase-9 enhanced in the LPS-stimulated RAW264.7 macrophage cells. It inhibited inhibitory κB-α degradation and enhanced NF-κB promoter activity in the stimulated macrophage cells. It was able to suppress phosphorylation of c-Jun NH(2)-terminal kinase 1/2 (JNK1/2) and activation of c-Jun promoter activity in the stimulated macrophage cells. In brief, n-propyl gallate possesses anti-inflammatory activity via down-regulation of NF-κB and JNK pathways.

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Section: Discussionmentioning

confidence: 99%

Anti-inflammatory Activity of n-Propyl Gallate Through Down-regulation of NF-κB and JNK Pathways

et al. 2010

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“…In addition to EGCG and quercetin, many other polyphenols and polyphenolic extracts have been found to inhibit the expression of COX-2, PGE 2 , iNOS and NO through the NF-κB pathway in LPS-activated RAW 264.7 cells. This includes apigenin, luteolin, luteolin-7-glucoside, prodelphinidin B-4 3′-O-gallate from green tea, 6-MITC from Wasabi japonica, oroxylin A from a traditional Chinese herb, and compound K, a metabolite of ginsenoside Rb1, itself a main constituent of the root Panax ginseng (Chen, Yang, & Lee, 2000;Hou, Luo, et al, 2007;Hu & Kitts, 2004;Liang et al, 1999;Park et al, 2005;Uto, Fujii & Hou, 2005a;Uto, Fujii, & Hou, 2005b). Interestingly, genistein and resveratrol exhibited a significant but low inhibition of the NF-κB pathway in LPS-activated murine macrophages, suggesting the action of these polyphenols occurs through alternative mechanisms (Murakami, Matsumoto, Koshimizu, & Ohigashi, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…The preparation of cell lysates was conducted according to Hou, Luo, et al (2007); with minor modifications. Initially, RAW 264.7 cells (1 × 10 6 per flask) were incubated for 24 h at 37°C in 25 cm 2 flasks.…”

Section: Preparation Of Cell Lysatesmentioning

confidence: 99%

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Native Australian fruit polyphenols inhibit COX-2 and iNOS expression in LPS-activated murine macrophages

Tan

Hou

Konczak

et al. 2011

Food Research International

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“…Measurement of PGE 2 production PGE 2 in the culture medium was measured with a PGE 2 enzyme immunoassay kit (Cayman Co., St. Luris, MO, USA) according to manufacturer's manual [12]. In brief, RAW 264.7 cells (5×10 5 cells) were seeded into each well of 6-well plates.…”

Section: Measurement Of Ros Productionmentioning

confidence: 99%

Antioxidant and anti-inflammatory activities of loquat (Eriobotrya japonica) tea

Zar

Sakao²,

Hashimoto

et al. 2013

FFHD

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Background: Fresh loquat leaves contain several kinds of flavonoids and have been reported to have preventive effects against some human diseases such as diabetes, coughs and ulcers,. Recently, fresh loquat leaves in Japan were processed to a beverage, called loquat tea, after the fresh leaves are roasted at 350C for 30 minutes. However, the scientific evidence supporting the functions of these processed leaves is still minimal.Objective: The aim of this study is to investigate the antioxidant and anti-inflammatory activities of roasted loquat tea extract (LTE) in vitro and in culture cells. Methods:Bioactive fractions of LTE were separated by column chromatograph. Antioxidant activities were determined by DPPH and ROS assay. Pro-inflammatory mediators cyclooxygenase-2 (COX-2) and prostaglandin E 2 (PGE 2 ) were determined by Western blot and ELISA assay, respectively. Chemical quantification and characterization were analyzed by HPLC, FR-IR, and NMR. Phenolic content was measured by Folin-Ciocalteu assay. Results:The results showed that loquat tea extract (LTE) possessed stronger DPPH scavenging activity than fresh. Cellular data revealed that LTE inhibited the production of reactive oxygen species (ROS), and further suppressed the production of COX-2 and PGE 2 in lipopolysaccharide (LPS)-activated RAW 264.7 cells. Chemical quantification and characterization data indicated that LTE contained new bioactive phenolic components that were produced from the roasting processes of fresh loquat leaves. Conclusions: Loquat tea made from roasted loquat leaves contained new bioactive phenolic compounds that contribute to its antioxidant and anti-inflammatory activities.

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Prodelphinidin B-4 3′-O-gallate, a tea polyphenol, is involved in the inhibition of COX-2 and iNOS via the downregulation of TAK1-NF-κB pathway

Cited by 64 publications

References 45 publications

Anti-inflammatory Activity of n-Propyl Gallate Through Down-regulation of NF-κB and JNK Pathways

Anti-inflammatory Activity of n-Propyl Gallate Through Down-regulation of NF-κB and JNK Pathways

Native Australian fruit polyphenols inhibit COX-2 and iNOS expression in LPS-activated murine macrophages

Antioxidant and anti-inflammatory activities of loquat (Eriobotrya japonica) tea

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