Procyanidins inhibit tumor angiogenesis by crosslinking extracellular matrix

Abstract

The results suggest that PC may be important MMP inhibitors that can be used as therapeutic anticancer agents.

Crosslinking strategies for preparation of extracellular matrix-derived cardiovascular scaffolds

“…2 ), and angiogenesis-mediated tumor growth ( Fig. 3 ) [ 58 , 59 ]. Teissedre et al [ 60 ] reported that PC extracted from grapes and red wine have remarkable scavenging activities and could inhibit oxidative modification of LDL in vitro .…”

Crosslinking of saphenous vein ECM by procyanidins for small diameter blood vessel replacement

“…25,26 The present enzymatic hydrolyzing test revealed that the PC-crosslinked svECM substitutes could effectively resist collagenase-II (MMP-8) degradation, similar to that of GA-crosslinked, which can be attributed to hydrogen bond formation between PC and proline residues in collagen and elastin molecules among svECM. 9,11,15 In our study, it was found that the PC-crosslinked svECMs are stable and the ultimate tensile strength and elastic modulus were almost unchanged after soaking for 30 day in vitro. These results may suggest the long-term stability and viability of PCcrosslinked svECM and PC crosslinks.…”

“…To evaluate the resistance of crosslinked svECM substitutes to enzymatic hydrolysis, specimens were determined the initial dry weight, cut into pieces ($1 mm 3 ), well immersed in a 1000 U/mL collagenase-II (Sigma-alderich, with an activity of 386 U/mg solid)/D-Hanks solution (pH 7.4), and incubated at 37 C for 4 h under continuous shaking according to our established method. 9,11 Finally, the enzymatic hydrolysis was stopped by adding 50 mL 10 mM ethylenediaminetetraactic acid (EDTA; SIGMA No. E9884) solution and all residual specimens were taken out after filtering through a 0.45-lm cellulose filter to separate undissolved matrix from solubilized molecules of each group.…”

“…C-003-5C) and cultured according to published method. 11 The SMCs were obtained by isolating deendothelial bovine aorta tissue segments using 2 mg/mL collagense-II (Sigma-alderich, 386 U/mg solid)/D-Hanks solution (pH 7.4) for 1 h, cultured in the same conditions as HUVECs and used in experiments between 3 and 7 passage. The extracts were prepared by soaking crosslinked svECM substitutes in cell culture medium DMEM (low glucose, Invitrogen Gibco, 2 mL/cm 2 piece for 24 h at 37 C. After soaking, the extract was collected and stored at 4 C for further use.…”

“…In addition, it has shown antivirus, antibacterial, anticarcinogen, and antiinflammatory bioactivities as well as of the ability to inhibit platelet aggregation. 10,11 Moreover, PC does not have acute and subacute toxicity, and its metabolism ways have been…”