Procuring biologically active galactomannans from spent coffee ground (SCG) by autohydrolysis and enzymatic hydrolysis

Lignin-carbohydrate complex (LCC) is the biological macromolecule that has been demonstrated to exert multiple biological functions, including antioxidant, antiinflammation and anti-tumorigenesis, which support its broad application in the bioengineering field. However, it remains elusive the involvements of LCC in human neurological disorders, especially those with the overproduction of reactive oxygen species (ROS), such as spinocerebellar ataxias (SCAs). In this study, we found a previously undetermined anti-protein aggregation activity of LCC. Initially, two individual LCC preparations and carbohydrate-free lignin were isolated from the water-extracted waste residues of Chionanthus retusus (C. retusus) tender leaves. The chemical compositional analysis revealed that lignin (61.5%) is the predominant constituent in the lignin-rich LCC (LCC-L-CR), whereas the carbohydrate-rich LCC (LCC-C-CR) is mainly composed of carbohydrate (60.9%) with the xylan as the major constituent (42.1%). The NMR structural characterization showed that LCC-L-CR preparation is enriched in benzyl ether linkage, while phenyl glycoside is the predominant type of linkage in LCC-C-CR. Both LCC and lignin preparations showed antioxidant activities as exemplified by their abilities to scavenge free radicals in cultured mammalian cells and ROS in zebrafish. We further demonstrated a pronounced capability of LCC-L-CR in inhibiting the aggregation of expanded Ataxin-3, disease protein of SCA type 3, in human neuronal cells. Taken together, our study highlights the antioxidant and novel anti-protein aggregation activities of the C. retusus tender leaves-derived LCC.

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“…CR-TL (26.1%), indicating a labile portion of hemicellulose undergoes degradation after hot water extraction (Azhar et al, 2015;Gu et al, 2020).…”

Section: Tender Leaf and Its Derived Materialsmentioning

confidence: 99%

Isolation and Identification of a Novel Anti-protein Aggregation Activity of Lignin-Carbohydrate Complex From Chionanthus retusus Leaves

Pei

Chen

Chan

et al. 2020

Front. Bioeng. Biotechnol.

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“…Vero cells were seeded in a 24-well plate and incubated for 24 h. The cells were treated with EMC and stimulated with AAPH. The intracellular ROS scavenging and cytoprotective effects of EMC on AAPH-stimulated Vero cells were evaluated by the DCF-DA and the MTT assays, respectively [29,30]. In addition, the effect of EMC on AAPH-induced apoptosis was determined with the Hoechst staining assay according to the protocol described by Wang et al [31].…”

Section: In Vitro Assaymentioning

confidence: 99%

The Potential of Sulfated Polysaccharides Isolated from the Brown Seaweed Ecklonia maxima in Cosmetics: Antioxidant, Anti-melanogenesis, and Photoprotective Activities

et al. 2020

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Sulfated polysaccharides prepared from marine algae are potential ingredients in nutraceutical, pharmaceutical, and cosmeceutical industries. In the present study, the antioxidant, anti-melanogenesis, and photoprotective effects of sulfated polysaccharides obtained from Ecklonia maxima (EMC) were investigated to evaluate their potential in cosmetic. EMC was successfully prepared through Celluclast-assisted extraction and ethanol precipitation, and it contained 79.88% of sulfated polysaccharides that with 69.37% carbohydrates and 10.51% sulfate. EMC effectively suppressed 2,2-azobis(2-amidinopropane) hydrochloride (AAPH)-induced oxidative stress in vitro in Vero cells and in vivo in zebrafish. Furthermore, EMC significantly inhibited mushroom tyrosinase and reduced melanin synthesis in alpha-melanocyte-stimulating hormone-stimulated B16F10 cells. In addition, EMC remarkably attenuated photodamage induced by UVB irradiation in vitro in human keratinocytes (HaCaT cells) and in vivo in zebrafish. Furthermore, EMC effectively inhibited wrinkle-related enzymes and improved collagen synthesis in UVB-irradiated human dermal fibroblasts (HDF cells). These results indicate that EMC possesses strong antioxidant, anti-melanogenesis, and photoprotective activities, and suggest that EMC may be an ideal ingredient in the pharmaceutical and cosmeceutical industries.

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“…Generally, ROS are naturally produced during the aerobic metabolism and scavenged by the antioxidant defense system. However, the balance between ROS generation and scavenging could be broken down by the environmental stresses such as chemical, fine dust particles, and ultraviolet irradiation [2][3][4][5]. An excess of ROS could damage cellular organelles and biomacromolecules, including proteins, lipids, and nucleic acids [6].…”

Section: Introductionmentioning

confidence: 99%

Isolation, Characterization, and Antioxidant Activity Evaluation of a Fucoidan from an Enzymatic Digest of the Edible Seaweed, Hizikia fusiforme

et al. 2020

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The previous study suggested that the sulfated polysaccharides from Hizikia fusiforme (HFPS) possess strong antioxidant activity. The purpose of this study is to isolate fucoidan from HFPS and to investigate its antioxidant activity. A fucoidan (HFPS-F4) with a molecular weight of 102.67 kDa was isolated from HFPS. HFPS-F4 contains 99.01% of fucoidan (71.79 ± 0.56% of carbohydrate and 27.22 ± 0.05% of sulfate content). The fucoidan increased the viability of H2O2-treated Vero cells by 5.41, 11.17, and 16.32% at the concentration of 12.5, 25, and 50 μg/mL, respectively. Further results demonstrated that this effect act diminishing apoptosis by scavenging intracellular reactive oxygen species (ROS) via increasing the expression of the endogenous antioxidant enzymes, which was induced by elevating total nuclear factor (erythroid-derived 2)-like 2 (Nrf2) levels. In addition, the in vivo test results displayed that the pretreatment of fucoidan improved the survival rates and decreased heart-beating rate, ROS, cell death, and lipid peroxidation in H2O2-stimulated zebrafish. Taken together, these results demonstrated that fucoidan isolated from HFPS has strong in vitro and in vivo antioxidant activities and it could be utilized in pharmaceutical, nutraceutical, and cosmeceutical industries.

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Procuring biologically active galactomannans from spent coffee ground (SCG) by autohydrolysis and enzymatic hydrolysis

Cited by 54 publications

References 37 publications

Isolation and Identification of a Novel Anti-protein Aggregation Activity of Lignin-Carbohydrate Complex From Chionanthus retusus Leaves

Isolation and Identification of a Novel Anti-protein Aggregation Activity of Lignin-Carbohydrate Complex From Chionanthus retusus Leaves

The Potential of Sulfated Polysaccharides Isolated from the Brown Seaweed Ecklonia maxima in Cosmetics: Antioxidant, Anti-melanogenesis, and Photoprotective Activities

Isolation, Characterization, and Antioxidant Activity Evaluation of a Fucoidan from an Enzymatic Digest of the Edible Seaweed, Hizikia fusiforme

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