Procoagulant Disorders in Patients with Newly Diagnosed Pancreatic Adenocarcinoma

Abstract

Background and objectives: Cancer coagulopathy is thought to be partially due to the up-regulation of tissue factor (TF), thrombin-antithrombin complex (TAT) and soluble P-selectin (sP-selectin). The purpose of this study was to evaluate the clinical significance of TF, TAT and sP-selectin in patients with pancreatic cancer. Materials and methods: The study included 93 subjects: 73 newly diagnosed patients with pancreatic adenocarcinoma (42 with stage I-III and 31 with metastatic cancer (stage IV)) and a control group of 20 healthy subjects. Analyzed patients were hospitalized in the Department of Digestive Tract Diseases, Medical University of Lodz or in the Department of Digestive Tract Surgery, Silesian University, Katowice, Poland. All laboratory parameters were measured using ELISA procedures. Results: TF plasma levels were detectable in all patients and were significantly higher in metastatic cancer compared to stage I-III patients and the control group (p < 0.05). In patients with pancreatic adenocarcinoma, the median levels of TAT were also elevated compared to the control group. Moreover, patients with metastases had significantly higher TAT concentration compared to the I-III cancer group. On the other hand, only the metastatic patients group showed significantly higher plasma sP-selectin levels compared to the controls (p = 0.009), whereas there was no difference between localized and metastatic cancer patients. Conclusions: The coagulation disorders are present in the majority of patients with pancreatic adenocarcinoma already at the diagnosis stage and reflect cancer progression and spread.

Evaluation of four commercial ELISAs to measure tissue factor in human plasma

“…Several studies have reported that disease groups, such as cancer, bacterial, and viral infections, and cardiovascular diseases had increased levels of TF-like antigen measured by Imubind [ [47] , [48] , [49] , [50] , [51] , [52] , [53] , [54] , [55] , [56] , [57] ] or Quantikine [ 46 , 58 , 59 ] compared with healthy controls ( Table ). These studies report statistically significant increases in TF-like antigen in different disease groups using the Imubind and Quantikine ELISAs.…”

Coagulation parameters for the differential diagnosis of pancreatic cancer in the early stage: a retrospective study

“…As in other malignant tumors, some CC parameters have been reported to have diagnostic value for PC. The serum FIB level in patients with PC was significantly higher than that in healthy controls, and the FIB level in metastatic PC patients was further increased [ 28 ]; compared to patients with low-risk intraductal papillary mucinous neoplasms (IPMN), those with high-risk IPMN had a markedly elevated level of FIB [ 29 ]; most PC patients suffered from abnormal coagulation alteration at the time of diagnosis, and tissue factor (TF) and thrombin–antithrombin (TAT) had potential roles in differentiating metastatic PC [ 30 ]. Similar to previous studies, this study also found that some CC indicators, namely, APTT, FIB, and DD2, were abnormally altered in patients with PC compared with patients with BPD; among them, FIB and DD2 could efficiently differentiate PC and EPC according the ROC results.…”

Measurement of tissue factor-positive extracellular vesicles in plasma: strengths and weaknesses of current methods

“…There are several commercial human TF ELISAs, including IMUNBIND TF ELISA, Sekisui Diagnostics; ZYMUTEST TF ELISA, Aniara Diagnostics; Human TF ELISA, ab108903 Abcam Inc; Quantikine ELISA, R&D Systems. These ELISAs have been used by different investigators to measure levels of TF protein in plasma samples [16,[70][71][72][73][74][75][76][77][78][79][80]. We prepare plasma from untreated and lipopolysaccharidetreated whole human blood samples to generated negative and positive controls, respectively, for TFþ EVs.…”