Evaluation of four commercial ELISAs to measure tissue factor in human plasma

Sachetto, Ana Teresa Azevedo; Archibald, Sierra J.; Bhatia, Smita; Monroe, Dougald M.; Hisada, Yohei; Mackman, Nigel

doi:10.1016/j.rpth.2023.100133

Cited by 4 publications

(2 citation statements)

References 59 publications

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“…There are many different commercial ELISAs for both human and mouse TF. However, we found that 4 commercial ELISAs for human TF failed to detect TF in LPS stimulated human whole blood (Sachetto et al, RPTH, 2023, in press) 133 . Shi et al 126 used a mouse TF ELISA (ZC1B10, Cat# 2C-39071) to measure TF levels in plasma from endotoxemic mice but no controls were used to show the specificity of this ELISA.…”

Section: Accepted Manuscriptmentioning

confidence: 98%

“…However, we found that four commercial ELISAs for human TF failed to detect TF in LPS-stimulated human whole blood. 133 Shi et al 126 used a mouse TF ELISA (ZC1B10, Cat# 2C-39071) to measure TF levels in plasma from endotoxemic mice but no controls were used to show the specificity of this ELISA. Two of the studies 98 127 analyzed TF expression in macrophages using western blotting with an anti-TF antibody from Abcam (Cat# ab151748).…”

Section: Inflammasomes and Lps-induced Activation Of Coagulation In Micementioning

confidence: 99%

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Monocyte Tissue Factor Expression: Lipopolysaccharide Induction and Roles in Pathological Activation of Coagulation

Sachetto

Mackman

2023

Thromb Haemost

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The coagulation system is a part of the mammalian host defense system. Pathogens and pathogen components, such as bacterial lipopolysaccharide (LPS), induce tissue factor (TF) expression in circulating monocytes that then activates the coagulation protease cascade. Formation of a clot limits dissemination of pathogens, enhances the recruitment of immune cells and facilitates killing of pathogens. However, excessive activation of coagulation can lead to thrombosis. Here, we review studies on the mechanism of LPS induction of TF expression in monocytes and its contribution to thrombosis and disseminated intravascular coagulation. Binding of LPS to Toll-like receptor 4 on monocytes induces a transient TF expression that involves activation of intracellular signaling pathways and binding of various transcription factors, such as c-rel/p65 and c-Fos/c-Jun, to the TF promoter. Inhibition of TF in endotoxemia and sepsis models reduces activation of coagulation and improves survival. Studies with endotoxemic mice showed that hematopoietic cells and myeloid cells play major roles in the activation of coagulation. Monocyte TF expression is also increased after surgery. Activated monocytes release TF-positive extracellular vesicles (EVs) and levels of circulating TF-positive EVs are increased in endotoxemic mice and in patients with sepsis. More recently, it was shown that inflammasomes contribute to the induction of TF expression and activation of coagulation in endotoxemic mice. Taken together, these studies indicate that monocyte TF plays a major role in the LPS-induced activation of coagulation. Selective inhibition of monocyte TF expression may reduce pathologic activation of coagulation in sepsis and other diseases.

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Section: Accepted Manuscriptmentioning

confidence: 98%

Section: Inflammasomes and Lps-induced Activation Of Coagulation In Micementioning

confidence: 99%

Monocyte Tissue Factor Expression: Lipopolysaccharide Induction and Roles in Pathological Activation of Coagulation

Sachetto

Mackman

2023

Thromb Haemost

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Challenges with measuring tissue factor antigen and activity in human plasma

Mackman,

Sachetto

2024

Blood Vessels, Thrombosis & Hemostasis

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Biomarkers of bleeding and venous thromboembolism in patients with acute leukemia

Hisada,

Archibald,

Bansal

et al. 2023

Preprint

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Background: Coagulopathy and associated bleeding and venous thromboembolism (VTE) are major causes of morbidity and mortality in patients with acute leukemia. The underlying mechanisms of these complications have not been fully elucidated. Objectives: To evaluate the associations between biomarker levels and bleeding and VTE in acute leukemia patients. Patients/Method: We examined plasma levels of activators, inhibitors and biomarkers of the coagulation and fibrinolytic pathways in patients ≥18 years with newly diagnosed acute leukemia compared to healthy controls. Multivariable regression models were used to examine the association of biomarkers with bleeding and VTE in acute leukemia patients. The study included 358 patients with acute leukemia (29 acute promyelocytic leukemia [APL], 253 non-APL acute myeloid leukemia [AML] and 76 acute lymphoblastic leukemia [ALL]), and 30 healthy controls. Results: Patients with acute leukemia had higher levels of extracellular vesicle (EV) tissue factor (TF) activity, phosphatidylserine-positive EVs, plasminogen activator inhibitor-1 (PAI-1), plasmin-antiplasmin complexes, cell-free DNA and lower levels of citrullinated histone H3-DNA complexes compared to healthy controls. APL patients had the highest levels of EVTF activity and the lowest levels of tissue plasminogen activator among the acute leukemia patients. There were 41 bleeding and 37 VTE events in acute leukemia patients. High EVTF activity was associated with increased risk of bleeding (sHR 2.30, 95%CI 0.99-5.31) whereas high PAI-1 was associated with increased risk of VTE (sHR 3.79, 95%CI 1.40-10.28) in these patients. Conclusions: Our study shows alterations in several biomarkers in acute leukemia and identifies biomarkers associated with risk of bleeding and VTE.

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Evaluation of four commercial ELISAs to measure tissue factor in human plasma

Cited by 4 publications

References 59 publications

Monocyte Tissue Factor Expression: Lipopolysaccharide Induction and Roles in Pathological Activation of Coagulation

Monocyte Tissue Factor Expression: Lipopolysaccharide Induction and Roles in Pathological Activation of Coagulation

Challenges with measuring tissue factor antigen and activity in human plasma

Biomarkers of bleeding and venous thromboembolism in patients with acute leukemia

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