Processing efficacy in relation to microbial contamination of skin allografts from 723 donors

Pianigiani, Elisa; Ierardi, Francesca; Cuciti, C.; Brignali, S; Oggioni, Marco R.; Fimiani, Michele

doi:10.1016/j.burns.2009.04.020

Cited by 22 publications

(39 citation statements)

References 19 publications

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“…Based on our past experience with cell viability measurements, 7,30,31,28 we elaborated a corrected formula to express CSA cell viability, excluding other confounding factors (i.e., weight and area of the sample procured from the skin graft, mL of solvent, skin normalization to 100%) and detecting OD at 570 nm. 29 We propose that expressing viability in terms of corrected OD (570 nm) (corr OD570 nm=OD positive control -OD negative control) could be more appropriate and closer to real data.…”

Section: Siena Skin Bank Viability Testing Methodsmentioning

confidence: 99%

“…Skin banks should regulate all their activities, including procurement, data recording, processing, storage, distribution and import/export of tissues, [18][19][20][30][31][32][33] according to written operating procedures detailed in technical guidelines. According to international guidelines and national legislation, the safety requirements of donor skin (whether from cadavers or living donors) must be guaranteed by special procedures and quality standards.…”

Section: Donor Screening and Selectionmentioning

confidence: 99%

“…Antibiograms are performed in the case of positive results. [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32] According to a recent study by Pirnay et al, 32 an incubation time of 14 days allows an additional 16.9% of contaminated skin to be detected compared with the traditional 3-day incubation protocol. The authors also noted that 24% of the slow-growing bacteria detected after 14 days could be considered potentially pathogenic.…”

Section: Bacteriamentioning

confidence: 99%

“…Only critically contaminated skin allografts were discarded (e.g., polyresistant bacteria). Skin allografts contaminated by nonpathogenic agents (e.g., Staphylococcus epidermidis, Staphylococcus capitis and Propionibacterium acnes) can be reprocessed by glycerolization; 30 antibiotics are selected on the basis of antibiograms, and those negative after reprocessing can be issued for further use. In 2016, in our skin bank, 25/132 (19.3%) donors appeared to be contaminated by nonpathogenic bacteria (after reprocessing by glycerolization recovery procedure, the final discard rate for multiresistant contaminated allografts was 3/132 [2.3%]).…”

Section: Skin Discardmentioning

confidence: 99%

“…13,15,16,27,30,36 However, three main problems are encountered. First, tissue viability is difficult to measure in the laboratory: since in vivo setting conditions are very different, laboratory tests cannot achieve 100% accuracy.…”

Section: Assessment Of Cell Viability: State Of the Artmentioning

confidence: 99%

See 4 more Smart Citations

Current insights into skin banking: storage, preservation and clinical importance of skin allografts

Tognetti¹,

Pianigiani²,

Ierardi³

et al. 2017

BSAM

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Donor skin and dermal grafts are used in several types of loss of substance for different clinical purposes. As biological physiological medication, donor skin grafts can promote re-epithelization, shorten healing time, alleviate pain and protect dermal and subcutaneous structures such as cartilage, tendons, bones and nerves. Though a great variety of dermal matrices and skin equivalents, both synthetic and semisynthetic, are now available for wound treatment, viable human skin allografts remain an important therapeutic choice for extensive deep burns and hard-to-heal wounds. In such cases, viable skin allografts have significantly better clinical outcomes than unviable human-derived allografts or synthetic medications. The demand for human-derived skin bioproducts continues to be a reason for the existence of skin banks. Skin bank organization is complex and requires continuous updating. Careful donor selection, thorough microbiological and serological donor screening for transmissible diseases and rigorous quality control during tissue preparation are necessary to minimize the risk of transmission of pathogenic agents. Skin banks must also observe standardized reproducible procedures to ensure tissue traceability and biological safety in all phases of processing and to avoid new biological contamination. Constant training and periodic checks are needed to keep skin bank operators attentive and responsible. Finally, skin banks should guarantee collection and storage of highly viable skin. Here, we discuss available tissue storage methods and the different types of skin bioproducts.

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Section: Siena Skin Bank Viability Testing Methodsmentioning

confidence: 99%

Section: Donor Screening and Selectionmentioning

confidence: 99%

Section: Bacteriamentioning

confidence: 99%

Section: Skin Discardmentioning

confidence: 99%

Section: Assessment Of Cell Viability: State Of the Artmentioning

confidence: 99%

See 3 more Smart Citations

Current insights into skin banking: storage, preservation and clinical importance of skin allografts

Tognetti¹,

Pianigiani²,

Ierardi³

et al. 2017

BSAM

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show abstract

Skin: Specific Recovery and Processing Issues

Pianigiani

Pierce²

2012

Tissue and Cell Processing

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Influence of electron beam irradiation on extracellular matrix of the human allogeneic skin grafts

et al. 2021

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The nonviable allogeneic human skin grafts might be considered as the most suitable skin substitutes in the treatment of extensive and deep burns. However, in accordance to biological security such grafts require the final sterilization prior to clinical application. The aim of the study was to verify the influence of electron beam irradiation of three selected doses: 18, 25, and 35 kGy on the extracellular matrix of human skin. Prior to sterilization, the microbiological tests were conducted and revealed contamination in all examined cases. Individual groups were subjected to single electron beam radiation sterilization at proposed doses and then subjected to microbiological tests again. The results of microbiological testing performed for all irradiation doses used were negative. Only in the control group was a growth of microorganisms observed. The FTIR spectrometry tests were conducted followed by the histological evaluation and mechanical tests. In addition, cost analysis of radiation sterilization of individual doses was performed. The results of spectroscopic analysis, mechanical tests, and histological staining showed no significant changes in composition and

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Processing efficacy in relation to microbial contamination of skin allografts from 723 donors

Cited by 22 publications

References 19 publications

Current insights into skin banking: storage, preservation and clinical importance of skin allografts

Current insights into skin banking: storage, preservation and clinical importance of skin allografts

Skin: Specific Recovery and Processing Issues

Influence of electron beam irradiation on extracellular matrix of the human allogeneic skin grafts

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