Influence of electron beam irradiation on extracellular matrix of the human allogeneic skin grafts

Abstract

The nonviable allogeneic human skin grafts might be considered as the most suitable skin substitutes in the treatment of extensive and deep burns. However, in accordance to biological security such grafts require the final sterilization prior to clinical application. The aim of the study was to verify the influence of electron beam irradiation of three selected doses: 18, 25, and 35 kGy on the extracellular matrix of human skin. Prior to sterilization, the microbiological tests were conducted and revealed contamination in all examined cases. Individual groups were subjected to single electron beam radiation sterilization at proposed doses and then subjected to microbiological tests again. The results of microbiological testing performed for all irradiation doses used were negative. Only in the control group was a growth of microorganisms observed. The FTIR spectrometry tests were conducted followed by the histological evaluation and mechanical tests. In addition, cost analysis of radiation sterilization of individual doses was performed. The results of spectroscopic analysis, mechanical tests, and histological staining showed no significant changes in composition and

Laminar Biomaterial Composite of PVA Cryogel with Amnion as Potential Wound Dressing

“…However, these gels were still elastic and firm and did not damage when pulled. Additionally, according to the literature [54], the irradiation of human allogeneic skin grafts with a dose of about 35 kGy, although more expensive, possesses the highest decontaminating values against microorganisms and viruses. That is why the 35 kGy dose was chosen for procedures of amnion attachment to the PVA cryogel with simultaneous sterilization of the dressing.…”

Structure and properties of the acellular porcine cornea irradiated with electron beam and its in‐situ implantation

“…10 It has been used to sterilize ultra-fine porcine bladder matrix, 11 poly(methyl methacrylate), 12 diclofenac, and aclofenac. 13 Low doses of E-beam radiation are used to sterilize biomaterials, such as human skin (18, 25, and 35 kGy), 14 donor cornea (15-21 kGy), 15 collagen scaffold (20 kGy), 11 and recombinant human type III collagen phosphorylated choline hydrogel (17,19, or 21 kGy). 16 Medical devices are sterilized at doses of 15 or 25 kGy as described in ISO 11137.…”