Processing and biological evaluation of porous HA/poly(methyl methacrylate) hybrid composite

Tripathi, G. N. R.; Basu, Bikramjit

doi:10.1007/s12572-011-0032-0

Cited by 4 publications

(2 citation statements)

References 22 publications

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“…In a different processing approach, microporous HA scaffolds are prepared using PMMA as a sacrificial additive. 9 In the last decade, limited efforts have been invested to fabricate and characterize gelatin-HA scaffolds. [10][11][12][13] Such studies often reported either the fabrication aspects of these porous scaffolds, or the response of osteoblast/human mesenchymal stem cells on the scaffold under static or dynamic culture conditions.…”

Section: Introductionmentioning

confidence: 99%

Cryogenically cured hydroxyapatite–gelatin nanobiocomposite for bovine serum albumin protein adsorption and release

2013

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This research paper presents the first results on the protein adsorption and release kinetics and in vitro biodegradability of cryogenically cured hydroxyapatite-gelatin based micro/macroporous scaffolds (CHAMPS). While the adsorption and release of bovine serum albumin (BSA) protein exhibits steady state behavior over an incubation period of up to 10 days, Fourier transform infrared (FT-IR) analysis importantly confirms the absence of any change in the secondary structure of BSA proteins due to interaction with the CHAMPS scaffold. The compression properties of the CHAMPS scaffold with interconnected porosity (pore size y50-200 mm) is characterized by a non-linear stress-strain response with a strength close to 5 MPa and a maximum strain of up to 24%. The slow but systematic increase in weight loss over a period of 7 days as well as apatite layer formation indicates its good bioactivity. The extensive micro-computed tomography (micro-CT) analysis establishes cancellous bone-like highly interconnected and complex porous architecture of the CHAMPS scaffold. Importantly, the excellent adsorption (up to 50%) and release (up to 60% of adsorbed protein) of BSA has been uniquely attributed to the inherent porous microstructure of the CHAMPS scaffold. Overall, the present study provides an assessment of the interaction of protein with the gelatin-hydroxyapatite macroporous scaffold in vitro, as well as reporting for the first time the efficacy of such scaffolds to release 60% of BSA loaded onto the scaffold in vitro, which is significantly higher than earlier literature reports.

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Section: Introductionmentioning

confidence: 99%

Cryogenically cured hydroxyapatite–gelatin nanobiocomposite for bovine serum albumin protein adsorption and release

2013

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“…For developing porous scaffolds, it is important that they meet certain requirements of bone tissue engineering and integrate well with the bone healing process. Some of the important aspects that need to be considered while developing these scaffolds are: appropriate micro-and macroscopic structural morphology including pore size, pore interconnectivity, biocompatibility, osteoconductivity, mechanical strength and biodegradability [40]. The idea is that if an implanted porous ceramic is progressively replaced by natural bone, its biomechanical properties should more and more resemble with natural bone.…”

Section: Properties Of Porous Calcium Phos-phatementioning

confidence: 99%

Development of Porous Calcium Phosphate Bioceramics for Bone Implant Applications: A Review

Naqshbandi

Sopyan²,

Gunawan³

2013

MATS

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The present review briefly outlines the most recent patents and journals on various aspects of porous calcium phosphate bioceramics including techniques of preparation, properties and bone implant applications. Bioactive ceramics are a class of materials that have capability to bond directly with the host bone. These materials can be easily assimilated by the body and are considered to be biodegradable. Researches have revealed that artificial bones made from hydroxyapatite or a combination of hydroxyapatite (HA) and tricalcium phosphate (TCP) is a perfect substitute for natural bone owing to its excellent biocompatibility and properties close to that of human bone. Bioceramics made of HA are available in dense and porous forms. Several efforts on the fabrication of porous calcium phosphate bioceramics have been carried upon in the field of clinical orthopaedics. The realisation of these efforts can be observed from the fact that numerous patents have been filed on methods of preparing porous calcium phosphate bioceramics for bone implant applications. A number of porous HA ceramics have been developed for applications in both tissue engineering and drug delivery systems. Porous bodies are decomposable in human body and provide a surface for proliferation and growth of cells that are infiltrated from the surrounding tissues so that a new bone grows into the pores and prevents any movement or loosening of the implants. Consequently, these can be used for filling the damaged bone, repair of fractured bone and even can be used as hard tissue replacements. Several processing techniques have been employed for fabrication of porous scaffolds. Among prominent techniques are gel casting, slip casting, camphene-based freeze casting and polymeric-sponge method.

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Microengineered Polymer‐ and Ceramic‐Based Biomaterial Scaffolds: A Topical Review on Design, Processing, and Biocompatibility Properties

Ramalingam

Jabbari

Ramakrishna³

et al. 2013

Micro and Nanotechnologies in Engineering Stem Cells and Tissues

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Processing and biological evaluation of porous HA/poly(methyl methacrylate) hybrid composite

Cited by 4 publications

References 22 publications

Cryogenically cured hydroxyapatite–gelatin nanobiocomposite for bovine serum albumin protein adsorption and release

Cryogenically cured hydroxyapatite–gelatin nanobiocomposite for bovine serum albumin protein adsorption and release

Development of Porous Calcium Phosphate Bioceramics for Bone Implant Applications: A Review

Microengineered Polymer‐ and Ceramic‐Based Biomaterial Scaffolds: A Topical Review on Design, Processing, and Biocompatibility Properties

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